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The map label for this gene is rlmM [H]

Identifier: 157157061

GI number: 157157061

Start: 3120220

End: 3121320

Strand: Reverse

Name: rlmM [H]

Synonym: EcE24377A_3112

Alternate gene names: 157157061

Gene position: 3121320-3120220 (Counterclockwise)

Preceding gene: 157156642

Following gene: 157155644

Centisome position: 62.68

GC content: 52.23

Gene sequence:

>1101_bases
ATGAATAAGGTTGTATTGCTGTGCCGTCCGGGCTTTGAAAAAGAGTGCGCCGCAGAAATTACCGATAAAGCCGGCCAGCG
GGAAATTTTCGGTTTTGCCCGCGTGAAAGAGAATGCGGGTTATGTCATTTATGAATGTTATCAACCTGATGATGGCGATA
AGTTAATCCGTGAGCTGCCGTTCAGTTCATTAATTTTTGCCCGCCAGTGGTTTGTGGTGGGGGAACTCCTGCAGCATTTG
CCGCCAGAAGATCGTATTACCCCCATTGTCGGCATGTTACAGGGCGTAGTAGAGAAGGGCGGTGAACTGCGTGTTGAAGT
TGCCGATACCAACGAAAGCAAAGAGTTACTGAAATTCTGCCGTAAATTTACTGTTCCGCTACGCGCTGCCTTGCGCGATG
CCGGGGTGCTGGCGAACTATGAAACGCCGAAGCGTCCGGTTGTGCATGTATTCTTTATTGCACCAGGCTGCTGCTATACC
GGTTACTCATACAGCAACAATAATTCGCCGTTCTATATGGGCATTCCGCGCCTGAAATTTCCGGCAGATGCGCCGAGTCG
TTCCACGCTCAAACTGGAAGAGGCATTTCATGTGTTTATTCCTGCGGATGAGTGGGATGAACGCCTGGCGAACGGGATGT
GGGCGGTGGATTTAGGCGCTTGCCCTGGCGGCTGGACCTACCAACTGGTGAAGCGCAACATGTGGGTTTATTCCGTCGAC
AACGGCCCGATGGCGCAAAGTCTGATGGATACCGGACAGGTGACGTGGCTGCGGGAAGACGGTTTCAAATTCCGTCCGAC
GCGCAGCAATATCTCCTGGATGGTATGCGATATGGTTGAAAAACCGGCGAAAGTTGCGGCATTGATGGCGCAGTGGCTGG
TTAATGGCTGGTGCCGTGAAACCATTTTCAACCTCAAACTGCCGATGAAAAAACGCTACGAAGAAGTGTCACACAATCTG
GCGTATATTCAGGCACAGCTTGATGAACATGGCATAAATGCTCAGATTCAGGCGCGGCAGTTGTATCACGATCGCGAAGA
AGTGACGGTGCACGTCCGCCGCATCTGGGCTGCGGTGGGTGGTCGTCGCGACGAGCGATAA

Upstream 100 bases:

>100_bases
TTGTGGGCGTTTGTCACTCCGGTTGGCGGCGTGAGCTTCCTCGCGGGCTGGGCGTTAATGTTAGTTGGTGCTATCCGTTT
AAAGCGCAAGGGCGTAAGTC

Downstream 100 bases:

>100_bases
CAGGCAAGGCCGGGGCGTCGTCTCCGGCCTGCTACCCTTTCCGTCAGGCTGTTACCAAAGAAGTTGCAACCTGGCGCTCA
TCAGAAATAATGTGCGTTAC

Product: putative RNA 2'-O-ribose methyltransferase

Products: NA

Alternate protein names: 23S rRNA 2'-O-ribose methyltransferase rlmM [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIYECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHL
PPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDLGACPGGWTYQLVKRNMWVYSVD
NGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVCDMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNL
AYIQAQLDEHGINAQIQARQLYHDREEVTVHVRRIWAAVGGRRDER

Sequences:

>Translated_366_residues
MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIYECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHL
PPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDLGACPGGWTYQLVKRNMWVYSVD
NGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVCDMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNL
AYIQAQLDEHGINAQIQARQLYHDREEVTVHVRRIWAAVGGRRDER
>Mature_366_residues
MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIYECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHL
PPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDLGACPGGWTYQLVKRNMWVYSVD
NGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVCDMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNL
AYIQAQLDEHGINAQIQARQLYHDREEVTVHVRRIWAAVGGRRDER

Specific function: Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA [H]

COG id: COG2933

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family. RlmM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789171, Length=366, Percent_Identity=100, Blast_Score=765, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011224
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: NA

Molecular weight: Translated: 41906; Mature: 41906

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIYECYQPDDGDKLIRELP
CCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHEEEECCCCEEEEEEECCCCHHHHHHHCC
FSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELLKFC
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
RKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYTGYSYSNNNSPFYMGIPRLKF
HHHCHHHHHHHHHCCEEECCCCCCCCEEEEEEECCCCEECCCCCCCCCCCEEEECCCEEC
PADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDLGACPGGWTYQLVKRNMWVYSVD
CCCCCCCCEEEEECEEEEEECCCHHHHHHHCCEEEEEECCCCCCCCHHEECCCEEEEEEC
NGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVCDMVEKPAKVAALMAQWLVNGWCRE
CCHHHHHHHCCCCEEEEECCCCEECCCCCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHH
TIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINAQIQARQLYHDREEVTVHVRRIWAAVG
HHHHCCCCHHHHHHHHHCCCEEEEEEHHCCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHC
GRRDER
CCCCCC
>Mature Secondary Structure
MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIYECYQPDDGDKLIRELP
CCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHEEEECCCCEEEEEEECCCCHHHHHHHCC
FSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELLKFC
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
RKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYTGYSYSNNNSPFYMGIPRLKF
HHHCHHHHHHHHHCCEEECCCCCCCCEEEEEEECCCCEECCCCCCCCCCCEEEECCCEEC
PADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDLGACPGGWTYQLVKRNMWVYSVD
CCCCCCCCEEEEECEEEEEECCCHHHHHHHCCEEEEEECCCCCCCCHHEECCCEEEEEEC
NGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVCDMVEKPAKVAALMAQWLVNGWCRE
CCHHHHHHHCCCCEEEEECCCCEECCCCCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHH
TIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINAQIQARQLYHDREEVTVHVRRIWAAVG
HHHHCCCCHHHHHHHHHCCCEEEEEEHHCCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHC
GRRDER
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA