Click here to switch to the map view.

The map label for this gene is rna

Identifier: 157157029

GI number: 157157029

Start: 666870

End: 667676

Strand: Reverse

Name: rna

Synonym: EcE24377A_0633

Alternate gene names: 157157029

Gene position: 667676-666870 (Counterclockwise)

Preceding gene: 157155862

Following gene: 157157899

Centisome position: 13.41

GC content: 51.18

Gene sequence:

>807_bases
ATGAAAGCATTCTGGCGTAACGCCGCGTTGCTCGCGGTTTCTCTGCTTCCCTTCTCTTCTGCCAACGCCTTAGCGTTGCA
GGCAAAACAGTATGGCGATTTTGATCGCTATGTCCTGGCCCTCTCCTGGCAAACCGGATTTTGCCAGAGTCAACACGATC
GAAATCGTAACGAACGAGATGAATGTCGCCTGCAAACCGAAACGACCAACAAAGCTGATTTTCTGACCGTACATGGTCTG
TGGCCAGGATTGCCTAAATCGGTTGCTGCCCGTGGTGTTGATGAACGCCGCTGGATGCGCTTCGGTTGCGCTACTCGCCC
AATCCCGAATCTACCAGAAGCGCGCGCCAGCCGAATGTGTTCATCGCCGGAAACCGGATTATCACTGGAAACGGCCGCTA
AACTAAGTGAAGTCATGCCAGGAGCTGGCGGACGTTCCTGCCTGGAACGCTACGAATATGCCAAACACGGTGCCTGCTTT
GGTTTTGATCCGGACGCATACTTCGGTACGATGGTACGCCTGAATCAAGAAATTAAAGAGAGCGAAGCCGGAAAATTCCT
TGCGGATAATTACGGTAAAACAGTGAGCCGCCGTGACTTTGACGCCGCCTTTGCCAAAAGCTGGGGAAAAGAGAACGTGA
AAGCAGTTAAGCTAACGTGCCAGGGTAACCCTGCGTATTTGACTGAAATTCAGATCTCGATCAAAGCTGACGCCATCAAC
GCTCCGCTTTCTGCAAACTCATTCTTGCCACAACCTCACCCAGGTAACTGTGGCAAAACCTTTGTGATTGATAAAGCGGG
TTATTAA

Upstream 100 bases:

>100_bases
CGCTGAATAAATCATCACGCCCGCCAGGTAAGCCCACCTGGCGGGCTTTTTTATGATTTAATAGATAGTATTCAGTGTAT
GTATGAGTTCCACACCCATT

Downstream 100 bases:

>100_bases
TCATCTGATTTACAAAACACGCTAATTCGTCTTCCATAAACCAAGGCGATTAAGATTTATACATAATGCGCTGCATTGAG
GAATTCCCTGAATGTGACGC

Product: ribonuclease I

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGL
WPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISIKADAIN
APLSANSFLPQPHPGNCGKTFVIDKAGY

Sequences:

>Translated_268_residues
MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGL
WPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISIKADAIN
APLSANSFLPQPHPGNCGKTFVIDKAGY
>Mature_268_residues
MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGL
WPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISIKADAIN
APLSANSFLPQPHPGNCGKTFVIDKAGY

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family

Homologues:

Organism=Escherichia coli, GI1786828, Length=268, Percent_Identity=100, Blast_Score=561, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNI_ECOLI (P21338)

Other databases:

- EMBL:   M55687
- EMBL:   U82598
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JQ0777
- RefSeq:   AP_001258.1
- RefSeq:   NP_415144.1
- PDB:   2EA1
- PDB:   2PQX
- PDB:   2PQY
- PDB:   2Z70
- PDBsum:   2EA1
- PDBsum:   2PQX
- PDBsum:   2PQY
- PDBsum:   2Z70
- ProteinModelPortal:   P21338
- SMR:   P21338
- DIP:   DIP-10723N
- MINT:   MINT-1292710
- STRING:   P21338
- EnsemblBacteria:   EBESCT00000002289
- EnsemblBacteria:   EBESCT00000014693
- GeneID:   949065
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0603
- KEGG:   eco:b0611
- EchoBASE:   EB0849
- EcoGene:   EG10856
- eggNOG:   COG3719
- GeneTree:   EBGT00050000008958
- HOGENOM:   HBG416495
- OMA:   DSVVAQM
- ProtClustDB:   PRK10095
- BioCyc:   EcoCyc:EG10856-MONOMER
- Genevestigator:   P21338
- GO:   GO:0005737
- InterPro:   IPR001568
- InterPro:   IPR018188
- Gene3D:   G3DSA:3.90.730.10

Pfam domain/function: PF00445 Ribonuclease_T2; SSF55895 RNase_T2

EC number: =3.1.27.6

Molecular weight: Translated: 29618; Mature: 29618

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00530 RNASE_T2_1; PS00531 RNASE_T2_2

Important sites: ACT_SITE 78-78 ACT_SITE 152-152 ACT_SITE 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERD
CCCHHHHHHEEEEEECCCCCCCEEEEEHHHCCCCCEEEEEEECCCCCCCCHHHCCCCCHH
ECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMC
HHEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHC
SSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACFGFDPDAYFGTMVRLNQEIKE
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
SEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISIKADAIN
HHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEEEEECCCC
APLSANSFLPQPHPGNCGKTFVIDKAGY
CCCCCCCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQTGFCQSQHDRNRNERD
CCCHHHHHHEEEEEECCCCCCCEEEEEHHHCCCCCEEEEEEECCCCCCCCHHHCCCCCHH
ECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFGCATRPIPNLPEARASRMC
HHEEEECCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHHHC
SSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACFGFDPDAYFGTMVRLNQEIKE
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
SEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYLTEIQISIKADAIN
HHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEEEEECCCC
APLSANSFLPQPHPGNCGKTFVIDKAGY
CCCCCCCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249