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The map label for this gene is ptsA [H]

Identifier: 157156963

GI number: 157156963

Start: 4474097

End: 4476598

Strand: Reverse

Name: ptsA [H]

Synonym: EcE24377A_4487

Alternate gene names: 157156963

Gene position: 4476598-4474097 (Counterclockwise)

Preceding gene: 157157214

Following gene: 157156895

Centisome position: 89.9

GC content: 55.12

Gene sequence:

>2502_bases
ATGGCCCTGATTGTGGAATTTATTTGTGAGCTACCTAACGGCGTACATGCACGTCCGGCAAGCCACGTTGAAACGCTGTG
TAATACTTTTTCATCACAAATTGAGTGGCATAACCTGCGCACTGACCGCAAGGGCAACGCCAAAAGCGCCCTTGCGCTGA
TTGGCACCGATACGCTGGCGGGCGATAACTGCCAGTTACTGATTTCCGGGGCCGACGAACAGGAAGCGCACCAGCGTTTA
AGCCAATGGCTGCGCGATGAATTCCCCCACTGCGATGCGCCGCTGGCGGAAGTTAAATCTGACGAACTGGAACCACTGCC
GGTTTCACTGACCAATCTGAATCCGCAAATTATCCGCGCCCGCACCGTGTGCAGCGGTAGTGCAGGCGGCATTCTGACGC
CGATCTCTTCTTTAGATCTCAATGCGCTGGGTAATCTTCCCGCAGCCAAAGGCGTTGACGCCGAGCAATCCGCACTGGAA
AACGGCCTGACGCTGGTACTGAAAAACATTGAGTTTCGTCTGCTGGATAGCGACGGTGCCACCAGCGCGATTCTGGAAGC
GCACCGATCCCTGGCTGGCGATACTTCCCTGCGCGAACATTTACTGGCAGGCGTCAGCGCCGGATTAAGCTGCGCCGAAG
CGATTGTCACCAGTGCGAATCACTTTTGCGAAGAGTTCGCCCGTTCCAGCAGCAGCTACCTGCAAGAACGTGCCCTGGAC
GTACGCGACGTCTGCTTCCAGTTACTCCAGCAAATCTACGGTGAGCAACGCTTCCCGGCACCGGGCAAACTGACGCAGCC
CGCCATTTGTATGGCTGATGAACTGACTCCCAGCCAGTTCCTCGAACTGGATAAAAATCACCTCAAAGGTCTGTTACTGA
AAAGTGGCGGCACCACCTCACATACCGTAATTCTTGCCCGTTCGTTCAACATTCCGACACTGGTTGGTGTAGATATTGAT
GCCCTTACTCCGTGGCAGCATCAAACGATTTATATCGACGGCAACGCTGGGGCGATTGTGGTTGAGCCAGGAGAAGCCGT
AGCTCGTTATTATCAGCAAGAAGCCCGCGTACAGGACGCCCTGCGTGAGCAACAGCGTGTCTGGCTGACCCAACAAGCCC
GTACCGCTGACGGTATCCGCATTGAAATTGCCGCTAACATCGCTCACTCCGTGGAAGCGCAGGCCGCATTCGGCAATGGT
GCGGAAGGCGTTGGTTTGTTCCGCACTGAAATGCTCTATATGGATCGCACCAGTGCACCGGGCGAAAGCGAGCTGTACAA
CATTTTTTGTCAGGCGCTGGAGTCTGCCAACGGACGCAGCATTATTGTGCGCACTATGGACATTGGCGGCGACAAACCCG
TTGATTATCTGAACATTCCGGCAGAAGCTAACCCGTTCCTCGGTTATCGCGCTGTGCGTATTTATGAAGAATACGCCTCG
CTGTTCACCACACAACTACGGTCGATCCTGCGCGCCTCCGCTCACGGCAGCCTGAAAATCATGATCCCGATGATCTCCTC
AATGGAAGAGATCTTATGGGTGAAAGAAAAACTGGCGGAAGCCAAACAGCAACTGCGTAACGAACACATTCCGTTTGATG
AGAAGATCCAGCTTGGCATTATGCTGGAAGTACCGTCGGTGATGTTCATCATCGATCAATGCTGCGAAGAGATTGATTTC
TTTAGTATTGGTAGTAATGACCTGACGCAATATCTGCTGGCAGTGGATCGCGATAACGCTAAGGTTACTCGTCACTACAA
CAGCCTGAATCCGGCATTCTTGCGGGCGCTCGATTACGCCGTGCAGGCGGTGCATCGCCAGGGCAAATGGATTGGTCTGT
GCGGTGAGCTGGGAGCGAAAGGTTCCGTGCTGCCGTTGCTGGTCGGCTTAGGGCTGGATGAACTCAGCATGAGCGCACCA
TCAATTCCGGCGGCGAAAGCGCGGATGGCGCAACTTGATAGCCGTGAGTGCCGCAAGTTGCTCAACCAGGCAATGGCCTG
CCGTACTTCGCTGGAAGTGGAACACCTGCTGGCGCAATTCCGCATGACCCAACAGGACGCACCGCTGGTCACCGCCGAGT
GCATCACGCTGGAAAGTGACTGGCGCAGCAAAGAAGAAGTGCTCAAAGGCATGACCGATAACCTGCTGCTGGCGGGCCGC
TGCCGCTATCCGCGTAAACTGGAAGCCGACTTATGGGCGCGCGAGGCCGTTTTCTCTACCGGTCTGGGCTTTAGTTTTGC
CATTCCACACAGCAAATCAGAACACATTGAGCAATCCACCATCAGCGTGGCGCGTCTGCAAGCGCCGGTGCGCTGGGGCG
ATGATGAAGCGCAATTCATCATTATGTTAACCCTGAACAAACACGCTGCGGGCGATCAGCACATGCGCATTTTCTCGCGC
CTCGCTCGCCGCATCATGCACGAAGAATTCCGTAACGCGCTGGTTAACGCCGCCTCTGCCGACGCTATCGCCAGCCTGCT
GCAACATGAACTGGAACTGTAA

Upstream 100 bases:

>100_bases
TTACATTACATTTGTCCAGCTTTTGGCAGATTTGTCACATGGCAGGCCGTTGCCGTGCGCTCTTATCCTGTAAACAGAAA
ACCGTACCGGGAGAGAGGCA

Downstream 100 bases:

>100_bases
AAGGAAACATCATGGAACTGTATCTGGACACCGCTAACGTCGCAGAAGTCGAACGTCTGGCACGCATATTCCCCATTGCC
GGGGTGACAACTAACCCGAG

Product: multi-phosphoryl transfer protein 2

Products: NA

Alternate protein names: MTP 2; Phosphoenolpyruvate-protein phosphotransferase; Enzyme I-Ani; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-like phosphotransferase enzyme IIA component; PTS system fructose-like EIIA component [H]

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRKGNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRL
SQWLRDEFPHCDAPLAEVKSDELEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTSANHFCEEFARSSSSYLQERALD
VRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELTPSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDID
ALTPWQHQTIYIDGNAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNG
AEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYAS
LFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDF
FSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAP
SIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGR
CRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSR
LARRIMHEEFRNALVNAASADAIASLLQHELEL

Sequences:

>Translated_833_residues
MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRKGNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRL
SQWLRDEFPHCDAPLAEVKSDELEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTSANHFCEEFARSSSSYLQERALD
VRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELTPSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDID
ALTPWQHQTIYIDGNAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNG
AEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYAS
LFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDF
FSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAP
SIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGR
CRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSR
LARRIMHEEFRNALVNAASADAIASLLQHELEL
>Mature_832_residues
ALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRKGNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLS
QWLRDEFPHCDAPLAEVKSDELEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALEN
GLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTSANHFCEEFARSSSSYLQERALDV
RDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELTPSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDA
LTPWQHQTIYIDGNAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNGA
EGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYASL
FTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFF
SIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPS
IPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRC
RYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRL
ARRIMHEEFRNALVNAASADAIASLLQHELEL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI48994992, Length=833, Percent_Identity=99.639855942377, Blast_Score=1709, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=834, Percent_Identity=44.9640287769784, Blast_Score=693, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=458, Percent_Identity=39.5196506550218, Blast_Score=332, Evalue=6e-92,
Organism=Escherichia coli, GI1789193, Length=502, Percent_Identity=33.8645418326693, Blast_Score=289, Evalue=5e-79,
Organism=Escherichia coli, GI1787994, Length=227, Percent_Identity=29.5154185022026, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786951, Length=153, Percent_Identity=24.8366013071895, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 91798; Mature: 91667

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00369 PTS_HPR_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRKGNAKSALALIGTDTLA
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEEEECCCCC
GDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDELEPLPVSLTNLNPQIIRA
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEEECCCHHHEEE
RTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALENGLTLVLKNIEFRLLDSDGA
EEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHCCCEEEEECCEEEEECCCCC
TSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTSANHFCEEFARSSSSYLQERALD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC
VRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELTPSQFLELDKNHLKGLLLKSGGTTS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCHHHHCEEEEECCCCCC
HTVILARSFNIPTLVGVDIDALTPWQHQTIYIDGNAGAIVVEPGEAVARYYQQEARVQDA
EEEEEEEECCCCEEEECCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHH
LREQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEMLYMDRTSAP
HHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHEEEEECCCCC
GESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYAS
CHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
LFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGI
HHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
MLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYA
EEECHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCCEEHHCCCCCHHHHHHHHHH
VQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKL
HHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHCCHHHHHHH
LNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHCCCCCCEEEECC
CRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFI
CCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
IMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAASADAIASLLQHELEL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRKGNAKSALALIGTDTLA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEEEECCCCC
GDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDELEPLPVSLTNLNPQIIRA
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEEECCCHHHEEE
RTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALENGLTLVLKNIEFRLLDSDGA
EEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHCCCEEEEECCEEEEECCCCC
TSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTSANHFCEEFARSSSSYLQERALD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC
VRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELTPSQFLELDKNHLKGLLLKSGGTTS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCHHHHCEEEEECCCCCC
HTVILARSFNIPTLVGVDIDALTPWQHQTIYIDGNAGAIVVEPGEAVARYYQQEARVQDA
EEEEEEEECCCCEEEECCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHH
LREQQRVWLTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEMLYMDRTSAP
HHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCHHHHHHEEEEECCCCC
GESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYRAVRIYEEYAS
CHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
LFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNEHIPFDEKIQLGI
HHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
MLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYA
EEECHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCCEEHHCCCCCHHHHHHHHHH
VQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKL
HHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHCCHHHHHHH
LNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHCCCCCCEEEECC
CRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFI
CCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
IMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAASADAIASLLQHELEL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]