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The map label for this gene is dnaB [H]

Identifier: 157156805

GI number: 157156805

Start: 3393100

End: 3394467

Strand: Reverse

Name: dnaB [H]

Synonym: EcE24377A_3371

Alternate gene names: 157156805

Gene position: 3394467-3393100 (Counterclockwise)

Preceding gene: 157156365

Following gene: 157155459

Centisome position: 68.17

GC content: 57.46

Gene sequence:

>1368_bases
ATGGCTGAAGTGTCAGTTCTCTGTGCATACGAAGCAGAGCAGGCCGTTCTGGGGGGCCTGATGCTGGAGAATGAACGCTG
GGATGAAGTGGTGCTGATGCTGAGTCCTGACGATTTTTATGTGGCTCAGCACCGGGCCATTTTCCGAGCCATGTCTGAAC
TGGCGGTGAATGGCCAGCCTCTGGACCTGATTACACTGTCAGAGCATCTGGAACATCAGGGCAGGCTGGACGTACTGGGC
GGTTTTGCTTATCTGGCCGAGCTCAGCAAGAACACACCTTCTGCGGCCAACATCATGGCCTATGCAGGCATTGTGGTGGA
GAAGAGCGTTCTGCGTCAGTTACAGGCCACCGGAAACACCCTGATTGCTGATGTGGCGGCACCGAACGCCACATCACGGG
ATGTGCTGGATACAGCAGAGCGGCGTCTGTTTGCGCTTGCTCAGAACAGGACGCTCCGGGAGCGCACGGAAGCCTCTCTT
TCATCCGCGCTCGATACCGTTCTGCAACAACTGGAAAGTGTGCTCGGTTCCGGCGGTGTGACCGGTACCCCCACGGGATT
TGACTGTCTGGATGAAATGACCTGTGGCCTGCAGCCCGGTGACCTGGTCCTGCTGGCTGCCCGTCCGTCAATGGGGAAAA
CTTCTCTGGCGCTGTCCATGTGTCTCAGCGCCGCCCGGCGACGGACAGAAGAGAGCGTTCTGGTATTCAGCATTGAGATG
CCGAAGGAGCAACTGATTCTGCGCATGCTTTCCATGCTGGGACGTGTGGAACTGAATCATCTGCGCAGTGGCAACATGGA
TGATGAGGACTGGGCGAGGGTGTCATCTGCGGTTGGCATGGCGCTGGGTGACGGAGGAATGGGGTCTCTGGGGGAGCGCC
TGATAATTGATGACTGCAGCCAGCAGACACCGTCATCACTGCGTGCCAGTGCCCGCCGTTATACCCGCCTGTACGGTAAA
CCCGCTCTGATTATGGTGGATTATCTGCAGCTGATTCGTGCGCCGGAGCTGGAGAACCGCACTCAGGAAATTGCTGAAAT
TTCCCGTTCCCTGAAAGCGCTGGGGAAGGAGCTGGGATGCCCGGTGCTGGCCCTCTCCCAGCTTAACCGTCAGGTGGAAC
AGCGTGCAGATAAACGCCCCAATAACGGTGACCTGCGTGATTCCGGGGCACTGGAGCAGGATGCTGACCTGATTATGTTT
ATTTACCGCGACGAGGTCTATAACCCCGATTCACTGGCAAAGGGGGAGGCTGAAATCATTGTCAGCAAGCAGCGTCAGGG
GCCCACAGGCACGGTGCGGGTCAGGTTCGACGGTCGTTACACACTGTTTACGCCCTCTGAAAATGACGGACAGGGAGGAT
ATGCGTGA

Upstream 100 bases:

>100_bases
ACAGCTACCGGGATTTTATTCCCGACAGCTGTACGTCCGGCACCCTTTTTCCTTTTTTCACATAGCCAGGTTTCCGGCAA
TCAGAGGGGGAGGGTACGCC

Downstream 100 bases:

>100_bases
TGGCACACAAACCACTGAATCTCGGGGCTGCCATTATGCAGCCCGGTCGTCAGGCCAGTGCTGCCGGTAATGTTGTCAGC
GCCAATGATATAAGACAGTA

Product: DnaB family helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MAEVSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQHRAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLG
GFAYLAELSKNTPSAANIMAYAGIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDTAERRLFALAQNRTLRERTEASL
SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLALSMCLSAARRRTEESVLVFSIEM
PKEQLILRMLSMLGRVELNHLRSGNMDDEDWARVSSAVGMALGDGGMGSLGERLIIDDCSQQTPSSLRASARRYTRLYGK
PALIMVDYLQLIRAPELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALEQDADLIMF
IYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDGQGGYA

Sequences:

>Translated_455_residues
MAEVSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQHRAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLG
GFAYLAELSKNTPSAANIMAYAGIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDTAERRLFALAQNRTLRERTEASL
SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLALSMCLSAARRRTEESVLVFSIEM
PKEQLILRMLSMLGRVELNHLRSGNMDDEDWARVSSAVGMALGDGGMGSLGERLIIDDCSQQTPSSLRASARRYTRLYGK
PALIMVDYLQLIRAPELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALEQDADLIMF
IYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDGQGGYA
>Mature_454_residues
AEVSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQHRAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGG
FAYLAELSKNTPSAANIMAYAGIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDTAERRLFALAQNRTLRERTEASLS
SALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLALSMCLSAARRRTEESVLVFSIEMP
KEQLILRMLSMLGRVELNHLRSGNMDDEDWARVSSAVGMALGDGGMGSLGERLIIDDCSQQTPSSLRASARRYTRLYGKP
ALIMVDYLQLIRAPELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALEQDADLIMFI
YRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDGQGGYA

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=441, Percent_Identity=54.1950113378685, Blast_Score=459, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49717; Mature: 49586

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQHRAIFRAMSELAVNGQP
CCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
LDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYAGIVVEKSVLRQLQATGNT
EEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
LIADVAAPNATSRDVLDTAERRLFALAQNRTLRERTEASLSSALDTVLQQLESVLGSGGV
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLALSMCLSAARRRTEESVLVFSIEM
CCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECC
PKEQLILRMLSMLGRVELNHLRSGNMDDEDWARVSSAVGMALGDGGMGSLGERLIIDDCS
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCC
QQTPSSLRASARRYTRLYGKPALIMVDYLQLIRAPELENRTQEIAEISRSLKALGKELGC
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PVLALSQLNRQVEQRADKRPNNGDLRDSGALEQDADLIMFIYRDEVYNPDSLAKGEAEII
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEE
VSKQRQGPTGTVRVRFDGRYTLFTPSENDGQGGYA
EECCCCCCCCEEEEEECCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AEVSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQHRAIFRAMSELAVNGQP
CCEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
LDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYAGIVVEKSVLRQLQATGNT
EEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
LIADVAAPNATSRDVLDTAERRLFALAQNRTLRERTEASLSSALDTVLQQLESVLGSGGV
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLALSMCLSAARRRTEESVLVFSIEM
CCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECC
PKEQLILRMLSMLGRVELNHLRSGNMDDEDWARVSSAVGMALGDGGMGSLGERLIIDDCS
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCC
QQTPSSLRASARRYTRLYGKPALIMVDYLQLIRAPELENRTQEIAEISRSLKALGKELGC
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PVLALSQLNRQVEQRADKRPNNGDLRDSGALEQDADLIMFIYRDEVYNPDSLAKGEAEII
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEE
VSKQRQGPTGTVRVRFDGRYTLFTPSENDGQGGYA
EECCCCCCCCEEEEEECCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]