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The map label for this gene is yiaJ [H]

Identifier: 157156720

GI number: 157156720

Start: 4054305

End: 4055117

Strand: Reverse

Name: yiaJ [H]

Synonym: EcE24377A_4071

Alternate gene names: 157156720

Gene position: 4055117-4054305 (Counterclockwise)

Preceding gene: 157155960

Following gene: 157157371

Centisome position: 81.43

GC content: 52.77

Gene sequence:

>813_bases
ATGGCGCAGGAAAAAGAGCGTCCAGCCGGAAGCCAGAGTCTGTTTCGCGGGTTGATGCTGATTGAGATTTTGAGCAACTA
TCCAAACGGTTGTCCGTTGGCGCATCTTTCGGAGCTGGCTGGTTTAAATAAGAGTACCGTCCATCGCTTATTGCAGGGAT
TACAGTCCTGCGGCTATGTGACCACCGCGCCCGCCGCAGGGAGTTACCGCCTGACTACCAAATTTATTGCCGTCGGGCAG
AAGGCGCTGTCTTCGCTGAATATCATTCATATCGCCGCTCCGCATCTTGAGGCACTGAACATCGCCACTGGTGAAACCAT
TAACTTCTCCAGCCGCGAAGACGATCACGCTATTTTGATTTATAAGCTGGAACCCACAACCGGGATGCTGCGAACCCGTG
CCTATATTGGCCAGCATATGCCGCTCTACTGTTCCGCAATGGGCAAGATCTACATGGCGTTTGGTCACCCGGACTACGTG
AAGTCATACTGGGAAAACCATCAGCATGAGATCCAGCCGTTAACCCGCAATACCATTACCGAGCTGCCCGCGATGTTCGA
CGAACTGGCGCACATTCGTGAAAGCGGAGCGGCGATGGACAGAGAAGAAAACGAACTCGGCGTCTCCTGTATTGCTGTTC
CGGTGTTTAATATTCATGGGCGGGTGCCGTACGCCGTGTCGATTTCGCTTTCGACATCACGTCTGAAACAGGTGGGGGAG
AAAAATCTCCTGAAACCACTGCGTGAAACCGCGCAGGCTATTTCTAATGAACTGGGATTTACTGTCAGGGACGACCAGGG
CGCGATCACATAA

Upstream 100 bases:

>100_bases
TATAAATTATCTGTCGAGATCGTGAACTACGGCACACTTTGCGCTACCATCAGGACGCGACAAAATGGGGAAAGAAGTGA
TGGGAAAAAAAGAGAACGAG

Downstream 100 bases:

>100_bases
CGCTTTGGACAAGTGCCAAAACTTTAACATTTCCTTCGTTGGATCAAAGCAGTAGGGACGCGCTCTCTGGCACTCTGCTG
TTTTAGTGCAAAGGAGTGAT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQ
KALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYV
KSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGE
KNLLKPLRETAQAISNELGFTVRDDQGAIT

Sequences:

>Translated_270_residues
MAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQ
KALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYV
KSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGE
KNLLKPLRETAQAISNELGFTVRDDQGAIT
>Mature_269_residues
AQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQK
ALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYVK
SYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEK
NLLKPLRETAQAISNELGFTVRDDQGAIT

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=270, Percent_Identity=98.8888888888889, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1786468, Length=251, Percent_Identity=33.8645418326693, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788131, Length=228, Percent_Identity=33.7719298245614, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786715, Length=251, Percent_Identity=29.4820717131474, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI87082359, Length=227, Percent_Identity=33.0396475770925, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790752, Length=219, Percent_Identity=31.0502283105023, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788581, Length=230, Percent_Identity=26.9565217391304, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI145693096, Length=217, Percent_Identity=29.0322580645161, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29746; Mature: 29615

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCE
TTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILI
EECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHEEEECCCCEECCCCCCCCCEEEE
YKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYVKSYWENHQHEIQPLTRNTIT
EEECCCCHHHHHHHHHCCCCCHHHHHHCCEEEEECCHHHHHHHHHCCHHHCCCHHHHHHH
ELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGE
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHH
KNLLKPLRETAQAISNELGFTVRDDQGAIT
HHHHHHHHHHHHHHHHHCCEEEECCCCCCC
>Mature Secondary Structure 
AQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCE
TTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILI
EECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHEEEECCCCEECCCCCCCCCEEEE
YKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYVKSYWENHQHEIQPLTRNTIT
EEECCCCHHHHHHHHHCCCCCHHHHHHCCEEEEECCHHHHHHHHHCCHHHCCCHHHHHHH
ELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGE
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHH
KNLLKPLRETAQAISNELGFTVRDDQGAIT
HHHHHHHHHHHHHHHHHCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]