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The map label for this gene is bglB [H]

Identifier: 157156633

GI number: 157156633

Start: 4219076

End: 4220470

Strand: Reverse

Name: bglB [H]

Synonym: EcE24377A_4230

Alternate gene names: 157156633

Gene position: 4220470-4219076 (Counterclockwise)

Preceding gene: 157157352

Following gene: 157159088

Centisome position: 84.75

GC content: 48.24

Gene sequence:

>1395_bases
ATGAAAGCATTTCCAGAAACATTTCTTTGGGGTGGCGCAACAGCTGCCAATCAGGTGGAAGGTGCCTGGCAGGAAGATGG
CAAAGGGATCTCGACCTCAGATTTACAGCCTCATGGCGTAATGGGAAAAATGGAACCGCGCATCCTGGGGAAAGAGAATA
TCAAAGATGTCGCCATCGATTTTTATCACCGTTACCCGGAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTG
CGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGAAGTCGAACCGAATGAAGCGGGGTTAGCGTTTTACGA
TCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGG
TGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCGATCACTTCGAGCATTACGCCCGCACGGTCTTTACTCGCTAC
CAACATAAAGTGGCGTTATGGCTGACGTTTAATGAAATCAACATATCGTTACACGAGCCATTCACGGGCGTGGGGCTGGC
AGAAGAGAGTGGCGAGGCGGAAGTTTATCAGGCTATCCACCATCAACTGGTTGCCAGTGCGCGGGCAGTTAAAGCCTGTC
ATAGCCTGCTCCCCGAAGCGAAAATCGGCAATATGCTTCTCGGTGGGCTGGTTTACCCCCTCACCTGCCAGCCACAGGAT
ATGTTGCAGGCCATGGAAGAGAACCGGCGCTGGATGTTCTTTGGTGATGTTCAGGCGCGTGGCCAGTATCCCGGCTATAT
GCAGCGTTTCTTCCGCGACCACAATATCACCATTGAGATGACTGAAAGTGACGCAGAAGATTTAAAACATACTGTAGATT
TCATCTCTTTTAGTTATTACATGACTGGTTGTGTTTCCCACGACGAAAGCATTAATAAAAATGCGCAGGGCAACATACTG
AATATGATCCCCAATCCGCATCTGAAAAGTTCAGAGTGGGGGTGGCAAATTGATCCGGTTGGATTACGGGTTCTGTTAAA
TACGCTTTGGGATCGTTATCAAAAACCGTTATTTATTGTCGAGAACGGATTAGGCGCAAAAGACAGCGTTGAAGCGGATG
GTTCGATACAGGACGATTATCGAATTGCCTATTTAAACGATCACCTGGTACAGGTAAATGAAGCGATTACCGATGGTGTG
GATATTATGGGGTACACCAGTTGGGGGCCAATTGATTTAGTCAGTGCATCTCATTCACAAATGTCTAAGCGCTACGGCTT
TATTTATGTGGATCGTGATGATAATGGCGAAGGAAGCCTCACAAGAACGCGTAAGAAAAGCTTCGGATGGTATGCAGAAG
TGATCAAAACGCGGGGGCTGTCATTAAAAAAATAA

Upstream 100 bases:

>100_bases
TAGCGATGATTTTACGGACGTATTACCCCACGGCACGGCGCAGATAAGCGCAGGTGAACCGCTGTTATCCATCATTCGCT
AACGATAAAAGGAGTTAATT

Downstream 100 bases:

>100_bases
CCAATAAAGCACCTTAATTATCGTCGCATTCAGAACAGTCTGGATGCGATACGTTAATTCTTTCTTTGCACCATAAAGGG
ATATTATGTTTAGACGAAAT

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKMEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCL
RISIAWARIFPQGDEVEPNEAGLAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
QHKVALWLTFNEINISLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLPEAKIGNMLLGGLVYPLTCQPQD
MLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNIL
NMIPNPHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQVNEAITDGV
DIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKK

Sequences:

>Translated_464_residues
MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKMEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCL
RISIAWARIFPQGDEVEPNEAGLAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
QHKVALWLTFNEINISLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLPEAKIGNMLLGGLVYPLTCQPQD
MLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNIL
NMIPNPHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQVNEAITDGV
DIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKK
>Mature_464_residues
MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKMEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCL
RISIAWARIFPQGDEVEPNEAGLAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
QHKVALWLTFNEINISLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLPEAKIGNMLLGGLVYPLTCQPQD
MLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNIL
NMIPNPHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQVNEAITDGV
DIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKK

Specific function: Can hydrolyze salicin and arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=482, Percent_Identity=29.6680497925311, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI110681710, Length=495, Percent_Identity=29.8989898989899, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI13273313, Length=487, Percent_Identity=29.1581108829569, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI24497614, Length=483, Percent_Identity=29.8136645962733, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI28376633, Length=478, Percent_Identity=27.4058577405858, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI2367270, Length=464, Percent_Identity=99.3534482758621, Blast_Score=969, Evalue=0.0,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=54.2194092827004, Blast_Score=540, Evalue=1e-154,
Organism=Escherichia coli, GI2367174, Length=474, Percent_Identity=51.0548523206751, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17552856, Length=487, Percent_Identity=29.1581108829569, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17539390, Length=483, Percent_Identity=28.1573498964803, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21356577, Length=488, Percent_Identity=27.6639344262295, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 52608; Mature: 52608

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKMEPRILGKENIKDVAID
CCCCCCHHCCCCCCHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
FYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAGLAFYDRLFDEMAQAGIKP
HHHHCCHHHHHHHHHCCCHHHHEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
LVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRYQHKVALWLTFNEINISLHEP
EEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCC
FTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLPEAKIGNMLLGGLVYPLTCQPQD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCEEEEEECCHHH
MLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNITIEMTESDAEDLKHTVDFISFSYY
HHHHHHCCCCEEEEECEECCCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHH
MTGCVSHDESINKNAQGNILNMIPNPHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIV
HHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCEEEE
ENGLGAKDSVEADGSIQDDYRIAYLNDHLVQVNEAITDGVDIMGYTSWGPIDLVSASHSQ
ECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCEEEECCCHHH
MSKRYGFIYVDRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKK
HHHHCCEEEEECCCCCCCCEEHHHHHHCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKMEPRILGKENIKDVAID
CCCCCCHHCCCCCCHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
FYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAGLAFYDRLFDEMAQAGIKP
HHHHCCHHHHHHHHHCCCHHHHEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
LVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRYQHKVALWLTFNEINISLHEP
EEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCC
FTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLPEAKIGNMLLGGLVYPLTCQPQD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCEEEEEECCHHH
MLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNITIEMTESDAEDLKHTVDFISFSYY
HHHHHHCCCCEEEEECEECCCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHH
MTGCVSHDESINKNAQGNILNMIPNPHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIV
HHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCEEEE
ENGLGAKDSVEADGSIQDDYRIAYLNDHLVQVNEAITDGVDIMGYTSWGPIDLVSASHSQ
ECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEECCCCCEEEECCCHHH
MSKRYGFIYVDRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKK
HHHHCCEEEEECCCCCCCCEEHHHHHHCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3034860; 7686882; 9278503; 3309161 [H]