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The map label for this gene is selU

Identifier: 157156550

GI number: 157156550

Start: 565087

End: 566181

Strand: Reverse

Name: selU

Synonym: EcE24377A_0540

Alternate gene names: 157156550

Gene position: 566181-565087 (Counterclockwise)

Preceding gene: 157156027

Following gene: 157155032

Centisome position: 11.37

GC content: 55.16

Gene sequence:

>1095_bases
ATGCAAGAGAGACACACGGAACAGGACTATCGTGCCCTGCTGATTGCTGATACGCCCATTATTGATGTTCGCGCCCCTAT
CGAGTTTGAGCAAGGCGCAATGCCCGCCGCTATCAATCTGCCGTTAATGAATAACGATGAACGCGTCGCCGTTGGCACCT
GTTATAAACAGCAAGGGTCAGACGCGGCGCTGGCGCTGGGTCATAAACTGGTGGCGGGTGAAATTCGTCAGCAGCGTATG
TACGCCTGGCGGGCAGCGTGCCTGCAAAATCCGCAAGGTATTCTCTGCTGCGCCCGTGGCGGTCAGCGCTCACATATTGT
GCAAAGCTGGTTGCATGCAGCGGGGATTGATTATCCGCTGGTGGAAGGCGGTTATAAGGCACTGCGCCAGACCACGATTC
AGGCGACTATTGAACTGGCACAAAAACCGATAGTGCTGATTGGCGGTTGTACCGGCTGCGGCAAAACGCTGTTAGTGCAA
CAACAGCCGAACGGTGTTGATCTGGAAGGGCTGGCGCGTCATCGCGGTTCGGCGTTTGGTCGCACCTTACAACCACAACT
TAGCCAGGCGAGTTTTGAAAACCTGCTGGCTGCCGAAATGCTAAAAACCGACGCCCGTCAGAATTTGCGCCTGTGGGTGC
TGGAAGACGAAAGCCGGATGATCGGTTCGAATCACCTGCCGGAATGCCTGCGCGAGCGAATGACTCAGGCGGCGATTGCG
GTGGTAGAAGATCCGTTTGAGATCCGTCTTGAGCGCCTGAACGAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCA
CGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCACGGACTTTCGGCGATCAAGCGTCGGCTGG
GGCTACAGCGCTATAACGAACTGGCTGCAAGGCTGGATGCAGCGCTGACAACGCAACTCGCAACCGGTAGCACCGACGGT
CATCTGGCCTGGCTGGTGCCGTTACTTAAAGAATATTACGACCCAATGTATCGCTATCAGCTTGAGAAAAAAGCGGAAAA
AGTTGTCTTTCGCGGTGAGTGGGCGGAAGTGGCGGAATGGGTTAAGGCGCGGTAA

Upstream 100 bases:

>100_bases
TTTTAGAAATTTTCGGCAACCCACGCAGTTAAAAATCAAGTTTCCTTGCGCGTTGGCGCGTAAAATAGCGCGCAAGCTGA
TTTGAGGTTCACTTTTAACT

Downstream 100 bases:

>100_bases
TGAGGGACGTATTTGTATTAGTCGCGACTTGATCTGACACATGGCCTTGAAAGGTTGAGAGTTACCGGTTTTGATATGAG
TGTCGAATCCTTATCCAAAA

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERVAVGTCYKQQGSDAALALGHKLVAGEIRQQRM
YAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPLVEGGYKALRQTTIQATIELAQKPIVLIGGCTGCGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLATGSTDG
HLAWLVPLLKEYYDPMYRYQLEKKAEKVVFRGEWAEVAEWVKAR

Sequences:

>Translated_364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERVAVGTCYKQQGSDAALALGHKLVAGEIRQQRM
YAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPLVEGGYKALRQTTIQATIELAQKPIVLIGGCTGCGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLATGSTDG
HLAWLVPLLKEYYDPMYRYQLEKKAEKVVFRGEWAEVAEWVKAR
>Mature_364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERVAVGTCYKQQGSDAALALGHKLVAGEIRQQRM
YAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPLVEGGYKALRQTTIQATIELAQKPIVLIGGCTGCGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLATGSTDG
HLAWLVPLLKEYYDPMYRYQLEKKAEKVVFRGEWAEVAEWVKAR

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Escherichia coli, GI1786712, Length=364, Percent_Identity=98.0769230769231, Blast_Score=736, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SELU_ECO24 (A7ZIR0)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461689.1
- ProteinModelPortal:   A7ZIR0
- SMR:   A7ZIR0
- STRING:   A7ZIR0
- EnsemblBacteria:   EBESCT00000018717
- GeneID:   5588169
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0540
- NMPDR:   fig|331111.3.peg.3074
- eggNOG:   COG2603
- GeneTree:   EBGT00050000010739
- HOGENOM:   HBG452364
- OMA:   NLPLMND
- ProtClustDB:   PRK11784
- BioCyc:   ECOL331111:ECE24377A_0540-MONOMER
- HAMAP:   MF_01622
- InterPro:   IPR001763
- InterPro:   IPR017582
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450
- TIGRFAMs:   TIGR03167

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: NA

Molecular weight: Translated: 41194; Mature: 41194

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERVAVGTCYKQQGS
CCCCCCCCHHEEEEEECCCEEEEECCCCCCCCCCCCEEECCEECCCCEEEEEEEHHCCCC
DAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCH
VEGGYKALRQTTIQATIELAQKPIVLIGGCTGCGKTLLVQQQPNGVDLEGLARHRGSAFG
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHCCHHCC
RTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNE
HHCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAARLDAALTTQLATGSTDGHLAWLVPLLKEYYDPMYRYQLEKKAEKVVFRGEWAEVAEW
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCHHHHHHH
VKAR
HHCC
>Mature Secondary Structure
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERVAVGTCYKQQGS
CCCCCCCCHHEEEEEECCCEEEEECCCCCCCCCCCCEEECCEECCCCEEEEEEEHHCCCC
DAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCH
VEGGYKALRQTTIQATIELAQKPIVLIGGCTGCGKTLLVQQQPNGVDLEGLARHRGSAFG
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHCCHHCC
RTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNE
HHCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAARLDAALTTQLATGSTDGHLAWLVPLLKEYYDPMYRYQLEKKAEKVVFRGEWAEVAEW
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCHHHHHHH
VKAR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA