Click here to switch to the map view.

The map label for this gene is rfaL [H]

Identifier: 157156511

GI number: 157156511

Start: 4114930

End: 4116183

Strand: Reverse

Name: rfaL [H]

Synonym: EcE24377A_4125

Alternate gene names: 157156511

Gene position: 4116183-4114930 (Counterclockwise)

Preceding gene: 157155796

Following gene: 157157713

Centisome position: 82.66

GC content: 33.33

Gene sequence:

>1254_bases
ATGTCGTTTTGTTGGAATGAAATTAATTCTGGTATAAAGTCTTTAATTCTCATATTATGTATTTTTTCTTTAATGACTTT
GTCTTTATGGGATGATGTTGCAACAAAGTTTCTTCATGCAGCTGGAATTATATCTGCATTGTATTTTCTTGCGACACCAA
AAAAAACAATAACTAATAATCCTACTTTGTTAATTTTCATCTCATTATGTCTTTTGGGTATCGTAAATATCATCTGGTAT
TCACATTATAAAGTTTCAGGCTCTGTTTATACCAATGCATATCGTGGCCCAATGGAAACTGGAAAAATTGCCTTGTGTAG
CGCTTTTATTTTCTTAGTTCTTTTTGCTAAAAATGAGATGAGAACAAAAATAAAATTTGGGAAACTAATTCTGTTCGCAT
CCCTGGCAACGCAGTTACTTTTTTTTGCGCATGCCATGTGGCAACATTTCTATTTAAACGTCGACCGTGTTGCATTATCA
GCTTCCCACGCTACAACAGCAGGCTACATCATCCTTTTTCCTTCTTTACTGGCATCAATTCTCATTTTAAAATCCGACTT
AAGACATAAAACAACATTATATACAATTAACTTCATGCTTAGCTTATGTGCTGTCATAGTAACTGAGACGCGTGCAGCCA
TATTAGTGTTTCCATTCTTTGCGTTAATATTAATCGTAATGGATAGTTATATTAATAAGCGAATTAATTATAAGTTATAT
TGTTTTATTACGATTGCATTATTAGCAGGTGTATTTTCTTTTAAAGATACATTGCTTATGAGAATGAATGACTTAAATAA
CGATTTAGTTAATTATTCGCATGATAACACCAGAACTTCAGTCGGTGCCCGTCTGGCAATGTATGAAGTTGGCTTAAAAA
CATATTCTCCAATAGGACAATCACTGGAAAAACGTGCGGAAAAAATACATGAACTAGAAGAAAAAGAGCCTAGATTGAGT
GGCGCTTTACCCTATGTAGATTCTCATTTGCATAACGATCTCATAGATACGTTATCAACGCGTGGTATTCCTGGAGTTGT
ATTAACAATTTTAGCATTTTCAGCAATACTCATATATGCCTTAAGAACTGCTAAAGAACCTTATATTTTAATCTTGCTTT
TTTCACTACTGGTAGTAGGACTAAGTGATGTAATACTCTTTTCTAAACCGGTTCCGACTGCTGTGTTTATCACCATAATA
TTGCTTTGTGCTTATTTTAAAGCACAATCGGACCAATGTTTATTAGAGAAGTAA

Upstream 100 bases:

>100_bases
TTATATTCTCTGGAGGATGGTAAGACTGGTAAATAGAAAAAAGAATAAACGTTAGATCTTGCATTAAATAACCCATCCTC
GTAGCATAGGTTGAAACTAT

Downstream 100 bases:

>100_bases
TGAATATGTGAAATAAAATCAGCAAGGAAGCTGATTTTATTTCAAAATTATCTATTAAAGAGTTGTAATTTAAATCATGG
CAGCGTTTTCTTCAATGAAC

Product: O-antigen polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGIISALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWY
SHYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
ASHATTAGYIILFPSLLASILILKSDLRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLY
CFITIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLS
GALPYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFITII
LLCAYFKAQSDQCLLEK

Sequences:

>Translated_417_residues
MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGIISALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWY
SHYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
ASHATTAGYIILFPSLLASILILKSDLRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLY
CFITIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLS
GALPYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFITII
LLCAYFKAQSDQCLLEK
>Mature_416_residues
SFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGIISALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYS
HYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSA
SHATTAGYIILFPSLLASILILKSDLRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLYC
FITIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSG
ALPYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFITIIL
LCAYFKAQSDQCLLEK

Specific function: Adds the O-antigen on the glucose group of LPS [H]

COG id: COG3307

COG function: function code M; Lipid A core - O-antigen ligase and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790052, Length=382, Percent_Identity=30.3664921465969, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007016 [H]

Pfam domain/function: PF04932 Wzy_C [H]

EC number: NA

Molecular weight: Translated: 46795; Mature: 46664

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGIISALYFLATPKKTITNN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
PTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKNEM
CHHHHHHHHHHHHHHHHHHHHCEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHH
RTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHHHHH
LILKSDLRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLY
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHH
CFITIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SLEKRAEKIHELEEKEPRLSGALPYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFITIILLCAYFKAQSDQCLLEK
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGIISALYFLATPKKTITNN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
PTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKNEM
CHHHHHHHHHHHHHHHHHHHHCEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHH
RTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHHHHH
LILKSDLRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLY
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHH
CFITIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SLEKRAEKIHELEEKEPRLSGALPYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFITIILLCAYFKAQSDQCLLEK
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1624462; 8041620; 9278503 [H]