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The map label for this gene is psuK [H]

Identifier: 157156425

GI number: 157156425

Start: 2446643

End: 2447584

Strand: Reverse

Name: psuK [H]

Synonym: EcE24377A_2463

Alternate gene names: 157156425

Gene position: 2447584-2446643 (Counterclockwise)

Preceding gene: 157157802

Following gene: 157155991

Centisome position: 49.15

GC content: 46.39

Gene sequence:

>942_bases
ATGCGCGAAAAGGATTATGTCGTAATTATAGGTTCGGCGAATATTGATGTCGCCGGATATTCACATGAATCATTAAATTA
TGCGGATTCAAATCCAGGTAAAATAAAATTTACGCCTGGTGGAGTAGGGCGCAATATTGCACAAAACCTGGCGTTGCTGG
GTAACAAAGCCTGGCTACTGAGCGCAGTAGGCAGTGATTTTTATGGTCAATCGCTGCTAACGCAAACCAATCAATCTGGC
GTTTATGTCGATAAATGCCTGATTGTGCCGGGAGAAAATACGTCTAGTTATTTATCATTACTCGATAATACCGGTGAAAT
GCTGGTTGCTATAAATGACATGAATATTAGCAACGCTATTACAGCTGAATATCTCGCACAGCACCGTGAATTTATTCAGA
GGGCAAAGGTCATTGTCGCAGACTGTAATATCAGTGAAGAGGCACTGGCATGGATTCTGGATAATGCCGCCAACGTACCC
GTATTTGTCGATCCGGTTTCAGCGTGGAAATGTGTCAAAGTGCGCGACCGTCTAAATCAGATCCACACTCTGAAGCCAAA
CCGCCTTGAAGCGGAAACACTGAGTGGGATTGCGCTGTCAGGGCGTGAAGATGTGGCAAAAGTTGCTGCCTGGTTCCATC
AACATGGCCTGAACCGACTGGTATTGAGCATGGGCGGCGATGGCGTTTATTACAGTGATATCAGCGGTGAAAGTGGCTGG
TCTGCCCCGATCAAAACCAATGTTATTAATGTTACCGGAGCGGGCGATGCCATGATGGCGGGACTTGCTTCGTGTTGGGT
AGACGGAATGCCGTTTGCCGAATCTGTTCGTTTCGCACAGGGATGTTCGTCAATGGCGCTCTCCTGTGAATACACCAATA
ACCCCGATTTATCGATTGCCAACGTTATATCGTTAGTGGAGAACGCAGAATGTCTGAATTAA

Upstream 100 bases:

>100_bases
GAGCACATGTTTAAATATGAACACATGTTTAAACCGTCTTTGCAGTATCTATATTCTTGTGGATTGACAAGGTTGGTAAT
TTTTTACCAGAGGACGTGCT

Downstream 100 bases:

>100_bases
AAATTTCCCCTGAATTATTACAAATTTCCCCGGAAGTGCAGGACGCTTTAAAAAACAAAAAACCGGTTGTGGCGCTGGAA
TCGACCATTATTTCTCACGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSG
VYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGW
SAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN

Sequences:

>Translated_313_residues
MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSG
VYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGW
SAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN
>Mature_313_residues
MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSG
VYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGW
SAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788491, Length=313, Percent_Identity=99.6805111821086, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1788484, Length=307, Percent_Identity=39.413680781759, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1790193, Length=237, Percent_Identity=23.6286919831224, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532905, Length=308, Percent_Identity=24.3506493506494, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24580669, Length=281, Percent_Identity=30.6049822064057, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 33672; Mature: 33672

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLL
CCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEE
SAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAI
EECCCCHHCCHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCHHH
TAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCVKVRDRLNQ
HHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHH
IHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGW
HHCCCCCCCCHHHHCCEEECCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCC
SAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIA
CCCCEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHH
NVISLVENAECLN
HHHHHHHHHHCCC
>Mature Secondary Structure
MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLL
CCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEE
SAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAI
EECCCCHHCCHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCHHH
TAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCVKVRDRLNQ
HHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHH
IHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGW
HHCCCCCCCCHHHHCCEEECCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCC
SAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIA
CCCCEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHH
NVISLVENAECLN
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]