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The map label for this gene is gldA

Identifier: 157156368

GI number: 157156368

Start: 4472309

End: 4473412

Strand: Reverse

Name: gldA

Synonym: EcE24377A_4485

Alternate gene names: 157156368

Gene position: 4473412-4472309 (Counterclockwise)

Preceding gene: 157156895

Following gene: 157155885

Centisome position: 89.83

GC content: 54.98

Gene sequence:

>1104_bases
ATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCC
GCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTTGCTCAATCCACTGTCGAGAAAAGCTTTAAAG
ATGCTGGACTGGTAGTAGAAATTGCGCCGTTTGGCGGTGAATGTTCGCAAAATGAGATCGACCGTCTGCGTGGCATCGCG
GAGACTGCGCAGTGTGGCGCAATTCTCGGTATCGGTGGCGGAAAAACCCTCGATACTGCCAAAGCACTGGCACATTTCAT
GGGTGTTCCGGTAGCGATCGCACCGACTATCGCCTCTACCGATGCACCGTGCAGCGCATTGTCTGTTATCTACACCGATG
AGGGTGAGTTTGACCGCTATCTGCTGTTGCCAAATAACCCGAATATGGTCATTGTCGACACCAAAATCGTCGCGGGCGCA
CCTGCACGTCTGTTAGCAGCGGGTATCGGCGATGCGCTGGCAACCTGGTTTGAAGCGCGTGCTTGCTCTCGTAGCGGCGC
GACCACCATGGCGGGCGGCAAGTGCACCCAGGCGGCGCTGGCACTGGCTGAACTGTGCTACAACACCCTGCTGGAAGAAG
GCGAAAAAGCGATGCTTGCCGCCGAACAGCATGTAGTAACCCCGGCGCTGGAGCGCGTGATTGAAGCGAACACCTATTTG
AGCGGCGTTGGTTTTGAAAGCGGCGGGCTGGCGGCGGCTCACGCGGTGCATAACGGCCTGACCGCTATCCCGGACGCGCA
TCACTATTATCACGGTGAAAAAGTGGCATTTGGTACGCTGACGCAGCTGGTTCTGGAAAACGCACCGGTTGAGGAAATCG
AAACCGTAGCTGCACTTAGCCATGCGGTAGGTTTGCCAATAACTCTCGCTCAACTGGATATTAAAGAAGATGTCCCGGCG
AAAATGCGAATTGTGGCAGAAGCGGCATGTGCAGAAGGTGAAACCATTCACAACATGCCTGGCGGCGCGACGCCAGATCA
GGTTTACGCCGCTCTGCTGGTAGCCGACCAGTACGGTCAGCGTTTCCTGCAAGAGTGGGAATAA

Upstream 100 bases:

>100_bases
CAACAAATGCTCAACACCCCTGCGGTAGAGTCAGCTATAGAGAAGTTCGAACACGACTGGAATGCCGCATTTGGCACTAC
TCATCTCTAAAGGAGCAATT

Downstream 100 bases:

>100_bases
CCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGTTTATCAAGCCTACGAAATC
TCTGCAATATATTGAATATG

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIA
ETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPA
KMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQYGQRFLQEWE

Sequences:

>Translated_367_residues
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIA
ETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPA
KMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQYGQRFLQEWE
>Mature_367_residues
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIA
ETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPA
KMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQYGQRFLQEWE

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family

Homologues:

Organism=Escherichia coli, GI87082352, Length=367, Percent_Identity=100, Blast_Score=747, Evalue=0.0,
Organism=Escherichia coli, GI1786815, Length=359, Percent_Identity=31.7548746518106, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLDA_ECOL6 (P0A9S6)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_756758.2
- ProteinModelPortal:   P0A9S6
- SMR:   P0A9S6
- EnsemblBacteria:   EBESCT00000044859
- GeneID:   1040105
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c4904
- GeneTree:   EBGT00050000009410
- HOGENOM:   HBG399530
- OMA:   CERTLFA
- ProtClustDB:   PRK09423
- BRENDA:   1.1.1.6
- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205
- PIRSF:   PIRSF000112

Pfam domain/function: PF00465 Fe-ADH

EC number: =1.1.1.6

Molecular weight: Translated: 38713; Mature: 38713

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00913 ADH_IRON_1; PS00060 ADH_IRON_2; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: BINDING 37-37 BINDING 121-121 BINDING 125-125 BINDING 127-127 BINDING 131-131 BINDING 171-171 BINDING 254-254 BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
IAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIAST
EECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCC
DAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFEAR
CCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCE
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAALS
ECCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHH
HAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQYGQ
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
RFLQEWE
HHHHHCC
>Mature Secondary Structure
MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEE
IAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIAST
EECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCC
DAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFEAR
CCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
ACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCE
SGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAALS
ECCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHH
HAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQYGQ
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHH
RFLQEWE
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157