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The map label for this gene is gntP

Identifier: 157156356

GI number: 157156356

Start: 4906902

End: 4908245

Strand: Reverse

Name: gntP

Synonym: EcE24377A_4919

Alternate gene names: 157156356

Gene position: 4908245-4906902 (Counterclockwise)

Preceding gene: 157159139

Following gene: 157156896

Centisome position: 98.57

GC content: 53.27

Gene sequence:

>1344_bases
ATGCATGTGCTTAACATTCTCTGGGTGGTATTCGGCATTGGTCTGATGCTGGTACTGAATTTGAAGTTCAAAATCAATTC
AATGGTGGCCTTGTTGGTGGCGGCGCTGTCCGTCGGGATGCTGGCGGGCATGGATTTGATGTCGCTGCTGCACACCATGA
AAGCGGGCTTCGGCAACACGCTGGGGGAACTGGCTATCATCGTGGTGTTCGGTGCGGTCATCGGTAAATTGATGGTCGAC
TCCGGCGCGGCTCACCAGATAGCGCATACGCTGCTGGCACGTCTCGGTCTGCGTTATGTACAGCTGTCGGTGATTATCAT
CGGCCTGATTTTCGGTCTGGCGATGTTCTATGAAGTGGCCTTTATCATGTTAGCACCGCTGGTTATTGTTATTGCCGCCG
AAGCTAAAATTCCGTTCCTGAAACTGGCGATCCCGGCAGTAGCAGCTGCCACTACCGCACATTCACTGTTCCCACCGCAG
CCGGGTCCGGTGGCGCTGGTGAATGCTTATGGCGCGGATATGGGGATGGTTTATATCTATGGCGTACTGGTGACGATCCC
AAGTGTCATCTGCGCAGGTCTGATCCTGCCGAAGTTTCTCGGCAATCTTGAGCGCCCAACGCCATCATTCCTGAAAGCAG
ATCAACCGGTAGATATGAATAATCTGCCCTCTTTCGGCGTTTCGATTCTGGTGCCGTTGATCCCGGCGATCATTATGATC
TCTACCACCATCGCCAATATCTGGCTGGTAAAAGATACCCCTGCGTGGGAAGTGGTTAACTTTATCGGTTCCTCGCCGAT
TGCAATGTTTATTGCGATGGTGGTTGCATTCGTACTCTTTGGCACCGCGCGCGGGCACGATATGCAGTGGGTAATGAACG
CTTTTGAAAGCGCGGTGAAAAGCATTGCAATGGTGATTCTGATCATCGGTGCAGGTGGCGTGCTGAAGCAGACCATCATC
GACACCGGCATCGGCGACACCATCGGCATGCTGATGTCCCACGGCAATATCTCGCCCTACATCATGGCATGGCTGATCAC
CGTACTGATTCGTCTGGCGACGGGTCAGGGTGTGGTTTCAGCGATGACCGCCGCCGGGATTATCAGTGCAGCAATCCTTG
ATCCAGCAACTGGTCAGCTGGTTGGCGTGAATCCGGCGCTGCTGGTACTGGCGACGGCTGCGGGTTCCAACACCCTCACC
CACATTAACGATGCCTCATTCTGGTTGTTCAAAGGTTACTTTGACCTGTCAGTAAAAGACACGTTGAAAACCTGGGGTCT
GCTGGAGCTGGTCAACTCCGTGGTTGGGCTGATTATTGTGCTGATTATTAGCATGGTAGCGTAA

Upstream 100 bases:

>100_bases
ATTGATCAGAGGATGTGACATTCATCGCAACAATGGTTGACCAATTTACATAACATATCGCCCAAAATAACAACGGTTCA
ACCAGGTAAGGTAAAACGTT

Downstream 100 bases:

>100_bases
AAGAGCACCTGAGCCTGCCATTGGCAGGCTCTGTTCGGATTGTCGGTAAAGTGCGCTTTGTTTATGCCAGATGCGACGCT
GACGCGTCTTATCTGGCCTA

Product: fructuronate transporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: Gluconate permease 3; Gnt-III system

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGMDLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVD
SGAAHQIAHTLLARLGLRYVQLSVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVDMNNLPSFGVSILVPLIPAIIMI
STTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVAFVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTII
DTGIGDTIGMLMSHGNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLATAAGSNTLT
HINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA

Sequences:

>Translated_447_residues
MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGMDLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVD
SGAAHQIAHTLLARLGLRYVQLSVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVDMNNLPSFGVSILVPLIPAIIMI
STTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVAFVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTII
DTGIGDTIGMLMSHGNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLATAAGSNTLT
HINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA
>Mature_447_residues
MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGMDLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVD
SGAAHQIAHTLLARLGLRYVQLSVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVDMNNLPSFGVSILVPLIPAIIMI
STTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVAFVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTII
DTGIGDTIGMLMSHGNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLATAAGSNTLT
HINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA

Specific function: High-affinity gluconate transporter with fairly broad specificity, including low affinity for glucuronate, several disaccharides, and some hexoses, but not glucose

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family

Homologues:

Organism=Escherichia coli, GI1790776, Length=447, Percent_Identity=100, Blast_Score=871, Evalue=0.0,
Organism=Escherichia coli, GI48994935, Length=438, Percent_Identity=40.6392694063927, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1790716, Length=435, Percent_Identity=40.4597701149425, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI2367370, Length=452, Percent_Identity=36.9469026548673, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788707, Length=446, Percent_Identity=31.8385650224215, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI48994939, Length=431, Percent_Identity=30.8584686774942, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789097, Length=442, Percent_Identity=30.7692307692308, Blast_Score=178, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GNTP_ECO57 (P0AC95)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C86130
- PIR:   H91288
- RefSeq:   NP_290937.1
- RefSeq:   NP_313307.1
- ProteinModelPortal:   P0AC95
- EnsemblBacteria:   EBESCT00000027969
- EnsemblBacteria:   EBESCT00000060461
- GeneID:   913673
- GeneID:   959732
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5919
- KEGG:   ecs:ECs5280
- GeneTree:   EBGT00050000009137
- HOGENOM:   HBG649941
- OMA:   GANIVAD
- ProtClustDB:   PRK10034
- BioCyc:   ECOL83334:ECS5280-MONOMER
- InterPro:   IPR003474
- TIGRFAMs:   TIGR00791

Pfam domain/function: PF02447 GntP_permease

EC number: NA

Molecular weight: Translated: 47139; Mature: 47139

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x21644118)-; HASH(0x22d5f1fc)-; HASH(0x22ffa814)-; HASH(0x231aecdc)-; HASH(0x22e092f8)-; HASH(0x2301734c)-; HASH(0x22b4dfdc)-; HASH(0x2305fce0)-; HASH(0x22ed32f8)-; HASH(0x23491dbc)-; HASH(0x231c9f10)-; HASH(0x22b6df14)-; HASH(0x22a317f0)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGMDLMSLLHTMKAGFGNT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQLSVIIIGLIFGLAMFYEVA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQPGPVALVNAYGADMGMVYIY
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHH
GVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVDMNNLPSFGVSILVPLIPAIIMI
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
STTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVAFVLFGTARGHDMQWVMNAFESAVK
HHHHHHEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSHGNISPYIMAWLITVLIRLATGQGVVS
HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHH
AMTAAGIISAAILDPATGQLVGVNPALLVLATAAGSNTLTHINDASFWLFKGYFDLSVKD
HHHHHHHHHHHHCCCCCCCEEECCHHHEEEEECCCCCCEEEECCCCEEEEECEECCCHHH
TLKTWGLLELVNSVVGLIIVLIISMVA
HHHHHHHHHHHHHHHHHHHHHHHHHHH
>Mature Secondary Structure
MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGMDLMSLLHTMKAGFGNT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQLSVIIIGLIFGLAMFYEVA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQPGPVALVNAYGADMGMVYIY
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHH
GVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVDMNNLPSFGVSILVPLIPAIIMI
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
STTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVAFVLFGTARGHDMQWVMNAFESAVK
HHHHHHEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSHGNISPYIMAWLITVLIRLATGQGVVS
HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHH
AMTAAGIISAAILDPATGQLVGVNPALLVLATAAGSNTLTHINDASFWLFKGYFDLSVKD
HHHHHHHHHHHHCCCCCCCEEECCHHHEEEEECCCCCCEEEECCCCEEEEECEECCCHHH
TLKTWGLLELVNSVVGLIIVLIISMVA
HHHHHHHHHHHHHHHHHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796