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The map label for this gene is yfiF [H]

Identifier: 157156261

GI number: 157156261

Start: 2867116

End: 2868153

Strand: Reverse

Name: yfiF [H]

Synonym: EcE24377A_2868

Alternate gene names: 157156261

Gene position: 2868153-2867116 (Counterclockwise)

Preceding gene: 157159134

Following gene: 157159266

Centisome position: 57.6

GC content: 56.45

Gene sequence:

>1038_bases
ATGAACGATGAAATGAAAGGTAAAAGCGGCAAGGTCAAAGTGATGTATGTCCGCAGTGATGATGATTCTGATAAACGTAC
CCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGACGGTGGCCGTCGCCCCGCCCGCGATG
ACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCACGGTTTCCCGCGCGCCGGGTGATGAGACG
CCGGAAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTATATTGATCCAGAAGTTTTGCGTCGTCAGCGTGCGGAAGA
AACCCGCGTCTACGGCGAAAATGCATGTCAGGCACTGTTCCAGAGCCGTCCGGAAGCGATTGTTCGCGCCTGGTTTATCC
AGAGCGTAACGCCACGTTTTAAAGAAGCCTTGCGCTGGATGGCGGCAAACCGCAAAGCGTACCATGTGGTGGATGAAGCG
GAATTGACAAAAGCGTCAGGCACGGAACATCACGGCGGCGTTTGCTTCTTGATCAAAAAGCGTAACGGTACAACCGTGCA
GCAGTGGGTAAGCCAGGCAGGCGCGCAGGATTGTGTACTGGCGCTGGAAAACGAATCTAACCCGCATAACCTGGGTGGCA
TGATGCGCAGCTGCGCGCACTTCGGCGTGAAAGGCGTTGTGGTGCAGGATGCGGCACTGCTGGAGTCGGGGGCGGCTATC
CGTACCGCAGAAGGCGGCGCAGAGCACGTTCAGCCGATTACTGGCGACAACATTGTTAACGTGCTGGATGATTTCCGTCA
GGCAGGTTACACCGTAGTGACCACTTCCAGCGAGCAGGGTAAACCGCTGTTCAAAACCAGTCTGCCAGCGAAAATGGTAC
TGGTGCTGGGTCAGGAATATGAAGGGTTACCGGATGCCGCACGCGATCCGAACGATCTGCGCGTGAAGATTGACGGTACG
GGCAACGTTGCCGGGCTGAATATCTCTGTCGCAACCGGCGTTTTGCTTGGTGAATGGTGGCGTCAGAATAAAGCCTGA

Upstream 100 bases:

>100_bases
TCAATAGGTATGATAGTCGCAGGCTTTGGTACGAAATCATGGCGAACCGCGCGGACTTCGGGTAATCTGCGCGCTTCGCG
CAGCGCTGGTGGAGAAAAGC

Downstream 100 bases:

>100_bases
TAAATCAGCCGGGTGGCAACTCTGCCATCCGGCATTTCCCCGCAAATTTACTCACTCTCTGCCGGTAATACTGGCATCCA
GTCAATCGGCGTCTCGCCAC

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDET
PEKADHGGISGKSYIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA

Sequences:

>Translated_345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDET
PEKADHGGISGKSYIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA
>Mature_345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDET
PEKADHGGISGKSYIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=345, Percent_Identity=99.7101449275362, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1790623, Length=247, Percent_Identity=27.9352226720648, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 37801; Mature: 37801

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPR
CCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DRKWEDSPWRTVSRAPGDETPEKADHGGISGKSYIDPEVLRRQRAEETRVYGENACQALF
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELTKASGTEHHGGVCFLIKK
HCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEC
RNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
CCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCEE
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEY
EECCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCEEEEEECCCC
EGLPDAARDPNDLRVKIDGTGNVAGLNISVATGVLLGEWWRQNKA
CCCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPR
CCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DRKWEDSPWRTVSRAPGDETPEKADHGGISGKSYIDPEVLRRQRAEETRVYGENACQALF
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELTKASGTEHHGGVCFLIKK
HCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEC
RNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
CCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCEE
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEY
EECCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCEEEEEECCCC
EGLPDAARDPNDLRVKIDGTGNVAGLNISVATGVLLGEWWRQNKA
CCCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]