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The map label for this gene is selA

Identifier: 157156233

GI number: 157156233

Start: 4072479

End: 4073870

Strand: Reverse

Name: selA

Synonym: EcE24377A_4089

Alternate gene names: 157156233

Gene position: 4073870-4072479 (Counterclockwise)

Preceding gene: 157156960

Following gene: 157155659

Centisome position: 81.81

GC content: 55.89

Gene sequence:

>1392_bases
ATGACAACCGAAACGCGTTCCCTCTATAGTCAACTTCCGGCTATTGATCGCTTATTGCGCGATAGCTCCTTCCTTTCTTT
GCGTGATACTTATGGTCACACCCGCGTGGTGGAATTGTTGCGTCAGATGCTCGACGAAGCGCGAGAAGTGATTCGTGGCA
GCCAGACGCTGCCTGCGTGGTGTGAAAACTGGGCGCAAGAAGTCGATGCCCGGTTGACGAAAGAAGCGCAGAGCGCGCTG
CGTCCGGTGATCAACCTGACGGGAACCGTGCTGCATACCAACCTTGGGCGAGCTTTACAGGCGGAAGCCGCGGTGGAAGC
CGTTGCGCAGGCTATGCGTTCGCCAGTGACCCTCGAGTACGATCTGGACGACGCTGGACGCGGACATCGCGATCGTGCGC
TGGCGCAGTTGCTGTGCCGCATTACGGGGGCGGAAGATGCCTGTATCGTTAATAACAATGCGGCGGCGGTGTTATTGATG
TTGGCGGCCACTGCCAGCGGGAAAGAGGTGGTGGTTTCTCGCGGCGAACTGGTGGAGATTGGCGGCGCGTTTCGTATTCC
CGATGTTATGCGTCAGGCAGGCTGCACCCTACACGAAGTAGGGACCACCAACCGCACGCACGCGAATGATTATCGTCAGG
CGGTGAATGAAAATACCGCACTGTTGATGAAAGTACATACCAGTAACTACAGCATTCAGGGGTTCACCAAAGCGATAGAT
GAATCGGAACTGGTGGCGCTCGGCAAAGAGCTGGATGTTCCCGTAGTGACTGATTTAGGCAGTGGCTCGCTGGTCGATCT
TAGCCAGTACGGTTTGCCGAAAGAGCCAATGCCGCAGGAGTTGATTGCGGCGGGCGTCAGTCTGGTGAGTTTCTCCGGCG
ACAAGTTGTTAGGCGGGCCGCAGGCAGGAATTATTGTTGGTAAAAAAGAGATGATCGCCCGCCTGCAAAGCCACCCGCTG
AAGCGTGCATTACGCGCGGATAAAATGACCCTCGCGGCGCTGGAAGCCACGTTGCGTCTTTATTTACACCCTGAAGCTCT
GAGTGAAAAATTACCGACCCTGCGCCTGCTTACCCGCAGCGCAGAGGTCATTCAAATCCAGGCACAACGTTTACAGGCCC
CCCTTGCCGCACATTACGGCGCGGAGTTTGCGGTACAGGTTATGCCATGTCTTTCGCAGATTGGCAGTGGTTCGCTGCCG
GTTGATCGCCTGCCGAGCGCGGCATTAACGTTTACACCCCATGATGGACGCGGTAGCCACCTTGAGTCATTAGCCGCCCG
CTGGCGTGAATTGCCAGTGCCGGTGATTGGTCGTATTTATGACGGACGATTGTGGCTGGATTTACGCTGCCTTGAAGATG
AGCAACGGTTTTTGGAGATGTTGTTGAAATGA

Upstream 100 bases:

>100_bases
TGATGCGCGATATGTCCTCCTGACCCATCTCACGTTACAATCCGTGGTTATGTTAAACGCCCTTCTCCGTGTGAGAGGGC
CTTGATCAGCCAGGTTTCCT

Downstream 100 bases:

>100_bases
TTATTGCGACTGCCGGACACGTTGACCACGGCAAAACAACCTTATTGCAGGCGATTACTGGCGTAAATGCTGACCGTCTG
CCGGAAGAAAAAAAGCGCGG

Product: selenocysteine synthase

Products: NA

Alternate protein names: Selenocysteine synthase; Sec synthase; Selenocysteinyl-tRNA(Sec) synthase

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQMLDEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSAL
RPVINLTGTVLHTNLGRALQAEAAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNENTALLMKVHTSNYSIQGFTKAID
ESELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEPMPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPL
KRALRADKMTLAALEATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLSQIGSGSLP
VDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRCLEDEQRFLEMLLK

Sequences:

>Translated_463_residues
MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQMLDEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSAL
RPVINLTGTVLHTNLGRALQAEAAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNENTALLMKVHTSNYSIQGFTKAID
ESELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEPMPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPL
KRALRADKMTLAALEATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLSQIGSGSLP
VDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRCLEDEQRFLEMLLK
>Mature_462_residues
TTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQMLDEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALR
PVINLTGTVLHTNLGRALQAEAAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLML
AATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNENTALLMKVHTSNYSIQGFTKAIDE
SELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEPMPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLK
RALRADKMTLAALEATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLSQIGSGSLPV
DRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRCLEDEQRFLEMLLK

Specific function: Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis

COG id: COG1921

COG function: function code E; Selenocysteine synthase [seryl-tRNASer selenium transferase]

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the selA family

Homologues:

Organism=Escherichia coli, GI2367248, Length=463, Percent_Identity=99.7840172786177, Blast_Score=937, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SELA_ECO24 (A7ZTD9)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001465068.1
- ProteinModelPortal:   A7ZTD9
- STRING:   A7ZTD9
- EnsemblBacteria:   EBESCT00000019311
- GeneID:   5588280
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4089
- NMPDR:   fig|331111.3.peg.1733
- eggNOG:   COG1921
- GeneTree:   EBGT00050000011558
- HOGENOM:   HBG564950
- OMA:   KRGDRDD
- ProtClustDB:   PRK04311
- BioCyc:   ECOL331111:ECE24377A_4089-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00423
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR018319
- InterPro:   IPR004534
- Gene3D:   G3DSA:3.40.640.10
- TIGRFAMs:   TIGR00474

Pfam domain/function: PF03841 SelA; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.9.1.1

Molecular weight: Translated: 50624; Mature: 50493

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQMLDEAREVIRGSQTLPAW
CCCCHHHHHHHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
CENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAEAAVEAVAQAMRSPVTLEY
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEE
DLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLMLAATASGKEVVVSRGELVEI
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCEEEE
GGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNENTALLMKVHTSNYSIQGFTKAID
CCCCCCHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCEEECHHHHHCC
ESELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEPMPQELIAAGVSLVSFSGDKLLGGP
CHHHHHHCCCCCCCEEEECCCCCEEEHHHCCCCCCCCCHHHHHCCHHHEEECCCCCCCCC
QAGIIVGKKEMIARLQSHPLKRALRADKMTLAALEATLRLYLHPEALSEKLPTLRLLTRS
CCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHCCCHHHHHHCC
AEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLSQIGSGSLPVDRLPSAALTFTPHDGRGSH
HHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEECCCCCCCHH
LESLAARWRELPVPVIGRIYDGRLWLDLRCLEDEQRFLEMLLK
HHHHHHHHHHCCCCHHCEEECCEEEEEEEECCHHHHHHHHHCC
>Mature Secondary Structure 
TTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQMLDEAREVIRGSQTLPAW
CCCHHHHHHHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
CENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAEAAVEAVAQAMRSPVTLEY
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEE
DLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLMLAATASGKEVVVSRGELVEI
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCEEEE
GGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNENTALLMKVHTSNYSIQGFTKAID
CCCCCCHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCEEECHHHHHCC
ESELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEPMPQELIAAGVSLVSFSGDKLLGGP
CHHHHHHCCCCCCCEEEECCCCCEEEHHHCCCCCCCCCHHHHHCCHHHEEECCCCCCCCC
QAGIIVGKKEMIARLQSHPLKRALRADKMTLAALEATLRLYLHPEALSEKLPTLRLLTRS
CCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHCCCHHHHHHCC
AEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLSQIGSGSLPVDRLPSAALTFTPHDGRGSH
HHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEECCCCCCCHH
LESLAARWRELPVPVIGRIYDGRLWLDLRCLEDEQRFLEMLLK
HHHHHHHHHHCCCCHHCEEECCEEEEEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA