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The map label for this gene is fecI

Identifier: 157155973

GI number: 157155973

Start: 4861742

End: 4862263

Strand: Reverse

Name: fecI

Synonym: EcE24377A_4870

Alternate gene names: 157155973

Gene position: 4862263-4861742 (Counterclockwise)

Preceding gene: 157158371

Following gene: 157156411

Centisome position: 97.64

GC content: 54.6

Gene sequence:

>522_bases
ATGTCTGACCGCGCCACTACCACAGCTTCCTTAACGTTCGAGTCGCTTTATGGCACACATCACGGCTGGTTGAAAAGCTG
GCTGACGCGCAAACTCCAGTCTGCTTTTGATGCAGATGACATTGCCCAGGACACTTTTTTGCGGGTAATGGTCAGCGAAA
CGCTCTCGACGATCCGCGATCCTCGCTCCTTCCTCTGCACTATCGCCAAACGCGTGATGGTGGACCTGTTTCGCCGAAAC
GCGCTGGAAAAAGCGTATCTGGAGATGCTGGCGCTTATGCCGGAGGGGGGAGCGCCTTCACCTGAGGAACGCGAAAGCCA
ACTCGAGACCCTACAACTCCTCGACAGCATGCTGGACGGGCTAAACGGCAAAACACGTGAAGCGTTTCTGCTTTCGCAAC
TGGATGGTCTGACATACAGCGAGATTGCGCACAAACTCGGTGTTTCCATCAGCTCCGTGAAAAAATACGTGGCGAAAGCC
GTCGAGCACTGCCTGCTGTTCCGTCTGGAGTATGGGTTATGA

Upstream 100 bases:

>100_bases
GTTAAAAATGTCTATTGGAAACAATTTTATTTCCAATTGTAATGATAACCATTCTCATATTAATATGACTACGTGATAAT
TAACTTTTGATGCACTCCGC

Downstream 100 bases:

>100_bases
ATCCTTTGTTAACCGATTCCCGCCGTCAGGCGCTGCGTTCAGCTTCCCACTGGTATGCCGTGCTAAGCGGCGAGCGCGTC
AGCCCACAACAGGAAGCGCG

Product: RNA polymerase sigma factor FecI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQDTFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRN
ALEKAYLEMLALMPEGGAPSPEERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
VEHCLLFRLEYGL

Sequences:

>Translated_173_residues
MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQDTFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRN
ALEKAYLEMLALMPEGGAPSPEERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
VEHCLLFRLEYGL
>Mature_172_residues
SDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQDTFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNA
LEKAYLEMLALMPEGGAPSPEERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKAV
EHCLLFRLEYGL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates the fec genes for iron dicitrate transport (Probable)

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily

Homologues:

Organism=Escherichia coli, GI1790746, Length=173, Percent_Identity=100, Blast_Score=354, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FECI_ECOLI (P23484)

Other databases:

- EMBL:   M63115
- EMBL:   U14003
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JV0111
- RefSeq:   AP_004783.1
- RefSeq:   NP_418713.1
- ProteinModelPortal:   P23484
- DIP:   DIP-9588N
- STRING:   P23484
- EnsemblBacteria:   EBESCT00000001719
- EnsemblBacteria:   EBESCT00000016968
- GeneID:   946839
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW4253
- KEGG:   eco:b4293
- EchoBASE:   EB0287
- EcoGene:   EG10291
- eggNOG:   COG1595
- GeneTree:   EBGT00050000011014
- HOGENOM:   HBG753452
- OMA:   REHPEIS
- ProtClustDB:   PRK09651
- BioCyc:   EcoCyc:PD00440
- BioCyc:   MetaCyc:PD00440
- Genevestigator:   P23484
- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 19481; Mature: 19349

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQDTFLRVMVSETLSTIRD
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
PRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPEERESQLETLQLLDSMLDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
LNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKAVEHCLLFRLEYGL
CCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQDTFLRVMVSETLSTIRD
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
PRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPEERESQLETLQLLDSMLDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
LNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKAVEHCLLFRLEYGL
CCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254251; 7610040; 9278503; 8052622