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The map label for this gene is rhtB [H]

Identifier: 157155928

GI number: 157155928

Start: 4334134

End: 4334754

Strand: Reverse

Name: rhtB [H]

Synonym: EcE24377A_4345

Alternate gene names: 157155928

Gene position: 4334754-4334134 (Counterclockwise)

Preceding gene: 157154863

Following gene: 157158817

Centisome position: 87.05

GC content: 52.01

Gene sequence:

>621_bases
ATGACCTTAGAATGGTGGTTTGCCTACCTGCTGACATCGATCATTTTAAGCCTGTCGCCAGGCTCTGGTGCAATCAACAC
TATGACCACCTCGCTCAACCACGGTTATCGCGGCGCGGTGGCGTCTATTGCTGGGCTTCAGACCGGACTGGCGATTCATA
TTGTGCTGGTTGGCGTGGGGTTGGGGACGCTATTTTCCCGCTCAGTGATTGCGTTTGAAGTGTTGAAGTGGGCAGGCGCG
GCTTACTTGATTTGGCTGGGAATCCAGCAGTGGCGCGCCGCTGGCGCAATTGATCTTAAATCGCTGGCCTCTACCCAATC
GCGCCGACATTTGTTCCAGCGCGCAGTTTTTGTGAATCTCACCAATCCCAAAAGTATTGTGTTTCTGGCGGCACTTTTTC
CACAATTCATCATGCCGCAACAGCCGCAAGTTATGCAGTATGTAGTGCTCGGCGTCACCACTATTGTGGTCGATATTATT
GTGATGATCGGTTACGCCACCCTTGCTCAACGGATTGCTCTATGGATTAAAGGACCAAAGCAGATGAAGGCGCTGAATAA
GATTTTCGGCTCGTTGTTTATGCTGGTGGGAGCGCTGTTAGCATCGGCGAGGCATGCGTGA

Upstream 100 bases:

>100_bases
GCCTCTTAAACCACGTAAAATCGTTTTTTTTAGCGTGCCTGACACAACGCTGCGACAGTAGCGTATTGTGGCACAAAAAT
AGACACACCGGGAGTTCATC

Downstream 100 bases:

>100_bases
AAAATAATGTCGGATGCGGCGTAAACGCCTTATCCGACTTACTCTGAAGACGCGTCTGGCATCACCGCGAAATAATCAAA
TGAATGCCAAATCCGGCAAA

Product: homoserine/homoserine lactone efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGA
AYLIWLGIQQWRAAGAIDLKSLASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQVMQYVVLGVTTIVVDII
VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA

Sequences:

>Translated_206_residues
MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGA
AYLIWLGIQQWRAAGAIDLKSLASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQVMQYVVLGVTTIVVDII
VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA
>Mature_205_residues
TLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAA
YLIWLGIQQWRAAGAIDLKSLASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQVMQYVVLGVTTIVVDIIV
MIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=206, Percent_Identity=99.0291262135922, Blast_Score=406, Evalue=1e-115,
Organism=Escherichia coli, GI1788099, Length=204, Percent_Identity=27.4509803921569, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22399; Mature: 22268

Theoretical pI: Translated: 11.20; Mature: 11.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSLASTQSRRHLFQRAVFVNL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEE
TNPKSIVFLAALFPQFIMPQQPQVMQYVVLGVTTIVVDIIVMIGYATLAQRIALWIKGPK
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QMKALNKIFGSLFMLVGALLASARHA
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVG
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSLASTQSRRHLFQRAVFVNL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEE
TNPKSIVFLAALFPQFIMPQQPQVMQYVVLGVTTIVVDIIVMIGYATLAQRIALWIKGPK
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QMKALNKIFGSLFMLVGALLASARHA
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]