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The map label for this gene is rlmI [H]

Identifier: 157155655

GI number: 157155655

Start: 1107440

End: 1108630

Strand: Reverse

Name: rlmI [H]

Synonym: EcE24377A_1082

Alternate gene names: 157155655

Gene position: 1108630-1107440 (Counterclockwise)

Preceding gene: 157155117

Following gene: 157156189

Centisome position: 22.26

GC content: 51.72

Gene sequence:

>1191_bases
ATGAGTGTACGTTTAGTGTTAGCCAAAGGGCGCGAAAAATCATTACTTCGTCGCCATCCGTGGGTCTTTTCCGGGGCCGT
TGCCCGCATGGAAGGTAAAGCCAGCCTCGGTGAAACCATCGATATTGTTGATCATCAGGGAAAATGGTTAGCACGCGGCG
CTTATTCACCAGCTTCGCAAATCCGGGCGCGCGTCTGGACGTTTGACCCGTCTGAGTCTATCGACATTGCTTTTTTTTCC
CGCCGTTTGCAACAAGCACAAAAATGGCGTGACTGGCTGGCGCAAAAAGATGGCCTCGACAGCTATCGTTTAATCGCCGG
AGAATCTGATGGCCTGCCGGGTATTACTATCGATCGTTTCGGTAATTTTCTGGTGCTGCAACTGCTGAGTGCTGGCGCAG
AATATCAGCGCGCGGCATTAATTAGTGCCCTGCAAACGCTGTACCCGGAATGTGCGATTTACGATCGCAGCGATGTTGCG
GTACGTAAAAAAGAAGGGATGGAGCTGACCCAGGGCCTCGTCACCGGCGAGTTGCCACCTGCCCTGCTGCCGATTGAAGA
ACACGGCATGAAACTGCTGGTGGATATTCAGCACGGACACAAAACGGGCTACTACCTGGACCAGCGTGATAGCCGCCTGG
CTACCCGCCGCTACGTTGAAAATAAACGTGTGCTGAACTGTTTCTCCTATACCGGTGGTTTCGCCGTATCGGCACTGATG
GGCGGTTGCAGCCAGGTTGTCAGCGTTGATACCTCCCAGGAAGCGCTGGATATTGCACGGCAGAACGTTGAGCTGAACAA
ACTGGATCTGAGCAAGGCTGAGTTTGTCCGTGATGATGTCTTTAAATTGCTGCGTACTTATCGCGATCGCGGTGAAAAAT
TTGACGTTATCGTGATGGACCCGCCGAAGTTTGTTGAGAATAAAAGCCAGTTGATGGGCGCGTGTCGTGGCTATAAAGAT
ATCAACATGCTGGCGATTCAGTTGCTGAATGAAGGCGGTATTCTCCTGACTTTCTCCTGTTCCGGTCTGATGACCAGCGA
TTTATTTCAGAAAATCATCGCGGATGCCGCAATTGATGCCGGCCGTGATGTACAATTTATAGAGCAGTTCCGTCAGGCAG
CCGATCATCCGGTGATCGCTACCTATCCGGAAGGGCTATATCTGAAAGGGTTTGCCTGTCGCGTCATGTAA

Upstream 100 bases:

>100_bases
CGACATTGCTGCTCTCCTTATCCTTAATGGACGCGTTGCAAATCCATGGAAAACTCCGGACAATGACGCCCATTTCTTTG
CATCGACAGAAAAGTAAATT

Downstream 100 bases:

>100_bases
CTTGAAATGTGGAATTTTACCCTTATATCAAAGGTAAAGATTTCCCGGGAGGTGACTATGATTGCCAGCAAATTCGGTAT
CGGCCAGCAGGTCCGCCATT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFS
RRLQQAQKWRDWLAQKDGLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALM
GGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKD
INMLAIQLLNEGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM

Sequences:

>Translated_396_residues
MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFS
RRLQQAQKWRDWLAQKDGLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALM
GGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKD
INMLAIQLLNEGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM
>Mature_395_residues
SVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSR
RLQQAQKWRDWLAQKDGLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVAV
RKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALMG
GCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDI
NMLAIQLLNEGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=396, Percent_Identity=99.4949494949495, Blast_Score=811, Evalue=0.0,
Organism=Escherichia coli, GI1787181, Length=241, Percent_Identity=29.4605809128631, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44358; Mature: 44226

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVDHQGKWLARGAYSPASQ
CCEEEEEECCCHHHHHHHCCCEEECHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCHHH
IRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGESDGLPGITIDRF
HEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEHHHH
GNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVAVRKKEGMELTQGLVTGELPP
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEHHHCCHHHHHHHHCCCCCC
ALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALM
CCCCHHHCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHHCCHHEEHHHCCCHHHHHHHH
GGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRDDVFKLLRTYRDRGEKFDVIVMD
HCHHHHEECCCCHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
PPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLFQKIIADAAIDA
CCHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
GRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM
CCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC
>Mature Secondary Structure 
SVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVDHQGKWLARGAYSPASQ
CEEEEEECCCHHHHHHHCCCEEECHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCHHH
IRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGESDGLPGITIDRF
HEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEHHHH
GNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVAVRKKEGMELTQGLVTGELPP
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEHHHCCHHHHHHHHCCCCCC
ALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALM
CCCCHHHCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHHCCHHEEHHHCCCHHHHHHHH
GGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRDDVFKLLRTYRDRGEKFDVIVMD
HCHHHHEECCCCHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
PPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLFQKIIADAAIDA
CCHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
GRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM
CCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA