| Definition | Escherichia coli E24377A, complete genome. |
|---|---|
| Accession | NC_009801 |
| Length | 4,979,619 |
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The map label for this gene is napA [H]
Identifier: 157155597
GI number: 157155597
Start: 2487182
End: 2489668
Strand: Reverse
Name: napA [H]
Synonym: EcE24377A_2505
Alternate gene names: 157155597
Gene position: 2489668-2487182 (Counterclockwise)
Preceding gene: 157157310
Following gene: 157154989
Centisome position: 50.0
GC content: 55.93
Gene sequence:
>2487_bases ATGAAACTCAGTCGTCGTAGCTTTATGAAAGCTAACGCCGTTGCGGCCGCTGCGGCGGCTGCCGGTCTCAGCGTGCCGGG CGTTGCCCGCGCCGTTGTTGGTCAGCAGGAAGCCATCAAATGGGATAAAGCGCCGTGCCGTTTCTGCGGTACTGGTTGCG GCGTTCTGGTCGGAACGCAGCAGGGACGTGTGGTGGCCTGTCAGGGCGACCCGGACGCACCGGTTAACCGTGGCCTGAAC TGCATTAAGGGCTATTTCCTGCCCAAGATCATGTACGGTAAAGACCGTTTGACGCAGCCGCTGCTGCGTATGAAAAACGG TAAATATGACAAAGAAGGCGAATTTACCCCAATCACCTGGGATCAGGCCTTCGATGTGATGGAAGAGAAATTCAAAACCG CCCTGAAAGAAAAAGGACCGGAATCGATCGGTATGTTCGGTTCTGGTCAGTGGACTATCTGGGAAGGTTATGCCGCGTCC AAGCTGTTTAAAGCGGGCTTCCGTTCGAACAACATCGACCCGAACGCACGTCACTGTATGGCGTCGGCAGTAGTTGGCTT TATGCGTACCTTTGGTATGGATGAGCCGATGGGCTGCTATGACGACATCGAGCAGGCTGACGCGTTTGTGCTGTGGGGCG CTAACATGGCGGAGATGCACCCGATCCTCTGGTCACGCATCACTAACCGTCGTCTCTCTAACCAGAACGTCACCGTGGCG GTGCTTTCTACCTACCAGCATCGTAGCTTCGAGCTGGCGGATAACGGCATCATCTTTACGCCGCAATCTGACCTGGTGAT CCTGAACTACATCGCCAACTATATCATTCAAAACAATGCGATAAATCAGGACTTCTTCAGCAAACACGTTAACCTGCGCA AAGGGGCGACGGACATCGGCTACGGTTTACGTCCGACCCATCCGCTGGAAAAAGCAGCGAAGAATCCAGGTTCTGACGCT TCCGAACCGATGAGCTTTGAAGATTACAAAGCCTTCGTTGCTGAGTATACGCTGGAAAAAACCGCTGAAATGACCGGCGT GCCGAAAGACCAGTTAGAACAACTGGCGCAGCTGTATGCCGATCCGAACAAGAAAGTCATCTCCTACTGGACGATGGGCT TCAACCAGCATACCCGTGGTGTGTGGGCCAACAACCTGGTCTACAACCTGCACCTGCTGACCGGCAAAATTTCCCAGCCG GGTTGCGGTCCGTTCTCCCTGACCGGGCAGCCTTCCGCGTGTGGTACTGCGCGTGAAGTGGGCACCTTTGCTCACCGTCT GCCTGCGGACATGGTGGTGACTAACGAGAAACATCGTGATATCTGCGAGAAGAAGTGGAATATCCCGAGCGACACCATTC CGGCGAAAATCGGTCTGCACGCGGTGGCACAAGACCGTGCACTGAAAGACGGCAAGCTGAATGTTTACTGGACCATGTGT ACCAACAACATGCAGGCCGGGCCGAACATTAATGAAGAGCGTATGCCGGGCTGGCGCGATCCGCGCAACTTCATCATCGT CTCCGATCCGTATCCGACAGTCAGTGCGCTGGCAGCCGACTTGATCCTGCCGACCGCAATGTGGGTAGAGAAAGAGGGCG CTTACGGTAACGCCGAACGCCGTACTCAGTTCTGGCGTCAGCAGGTACAGGCGCCAGGCGAAGCGAAATCGGATCTCTGG CAGTTAGTCCAGTTCTCCCGCCGCTTCAAAACTGAAGAAGTATGGCCGGAAGAACTGCTGGCGAAGAAACCGGAACTGCG TGGCAAAACGCTGTACGAAGTTCTGTATGCCACCCCCGAAGTGAGCAAATACCCTGTATCCGAACTGGCGGAAGATCAGC TGAACGATGAATCCCGCGAGCTGGGCTTCTATCTGCAAAAAGGGCTGTTCGAAGAGTACGCATGGTTTGGTCGCGGTCAC GGTCACGATCTGGCACCGTTCGATGACTACCACAAAGCGCGCGGTCTGCGCTGGCCGGTGGTGAACGGTAAAGAAACGCA GTGGCGTTACAGCGAAGGTAACGACCCGTACGTGAAAGCGGGCGAAGGCTATAAGTTCTACGGTAAACCGGATGGCAAAG CGGTGATCTTCGCACTGCCGTTCGAACCGGCGGCGGAAGCACCGGATGAAGAGTACGACCTGTGGCTCTCTACCGGACGC GTTCTGGAGCACTGGCACACCGGCAGTATGACTCGCCGTGTGCCGGAACTGCACCGCGCCTTCCCGGAAGCGGTCTTGTT TATTCACCCGCTGGATGCGAAAGCGCGCGATCTGCGCCGTGGCGACAAAGTGAAAGTGGTTTCTCGCCGTGGCGAAGTGA TCTCGATTGTTGAAACGCGCGGTCGTAACCGTCCGCCACAGGGCCTGGTGTACATGCCGTTCTTCGACGCCGCACAGCTG GTTAACAAACTGACGCTGGATGCGACCGATCCGCTCTCGAAAGAGACGGACTTCAAGAAGTGCGCAGTCAAACTGGAGAA GGTGTAA
Upstream 100 bases:
>100_bases AAACGCTGATCCAAACCATTGAGTCAGTACGCAACGTAGAGGGCGTGCTGGCGGTGTCGCTGGTTTATCACCAGCAGGAA GAGCAAGGTGAGGAAACACC
Downstream 100 bases:
>100_bases GCCATTATGTCCCGGTCAGCGAAACCTCAAAATGGTCGCCGCCGCTTTCTGCGCGATGTCGTTCGCACAGCAGGCGGGCT GGCTGCCGTGGGTGTGGCGC
Product: nitrate reductase catalytic subunit
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 828; Mature: 828
Protein sequence:
>828_residues MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKAPCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLN CIKGYFLPKIMYGKDRLTQPLLRMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVA VLSTYQHRSFELADNGIIFTPQSDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDA SEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHLLTGKISQP GCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPSDTIPAKIGLHAVAQDRALKDGKLNVYWTMC TNNMQAGPNINEERMPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLW QLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFYLQKGLFEEYAWFGRGH GHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGR VLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAAQL VNKLTLDATDPLSKETDFKKCAVKLEKV
Sequences:
>Translated_828_residues MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKAPCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLN CIKGYFLPKIMYGKDRLTQPLLRMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVA VLSTYQHRSFELADNGIIFTPQSDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDA SEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHLLTGKISQP GCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPSDTIPAKIGLHAVAQDRALKDGKLNVYWTMC TNNMQAGPNINEERMPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLW QLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFYLQKGLFEEYAWFGRGH GHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGR VLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAAQL VNKLTLDATDPLSKETDFKKCAVKLEKV >Mature_828_residues MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKAPCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLN CIKGYFLPKIMYGKDRLTQPLLRMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVA VLSTYQHRSFELADNGIIFTPQSDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDA SEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHLLTGKISQP GCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPSDTIPAKIGLHAVAQDRALKDGKLNVYWTMC TNNMQAGPNINEERMPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLW QLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFYLQKGLFEEYAWFGRGH GHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGR VLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAAQL VNKLTLDATDPLSKETDFKKCAVKLEKV
Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla
COG id: COG0243
COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]
Homologues:
Organism=Escherichia coli, GI1788534, Length=828, Percent_Identity=99.6376811594203, Blast_Score=1726, Evalue=0.0, Organism=Escherichia coli, GI3868721, Length=801, Percent_Identity=24.4694132334582, Blast_Score=209, Evalue=6e-55, Organism=Escherichia coli, GI3868720, Length=660, Percent_Identity=23.3333333333333, Blast_Score=122, Evalue=9e-29, Organism=Escherichia coli, GI3868719, Length=660, Percent_Identity=22.7272727272727, Blast_Score=112, Evalue=8e-26, Organism=Escherichia coli, GI87081797, Length=893, Percent_Identity=23.0683090705487, Blast_Score=103, Evalue=4e-23, Organism=Escherichia coli, GI1787870, Length=890, Percent_Identity=22.4719101123595, Blast_Score=101, Evalue=2e-22, Organism=Escherichia coli, GI171474008, Length=897, Percent_Identity=22.8539576365663, Blast_Score=88, Evalue=2e-18,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR009010 - InterPro: IPR006657 - InterPro: IPR006656 - InterPro: IPR006963 - InterPro: IPR006655 - InterPro: IPR010051 - InterPro: IPR006311 [H]
Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]
EC number: =1.7.99.4 [H]
Molecular weight: Translated: 93131; Mature: 93131
Theoretical pI: Translated: 8.02; Mature: 8.02
Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 4.1 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 4.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKAPCRFCGTGCGVLVGTQ CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCCCHHHHCCCCCEEEECC QGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLLRMKNGKYDKEGEFTPITW CCCEEEECCCCCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCH DQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAASKLFKAGFRSNNIDPNARHCM HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHH ASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVA HHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEE VLSTYQHRSFELADNGIIFTPQSDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIG EEECCCCCCEEECCCCEEECCCCCEEHHHHHHHHHHCCCCCCHHHHHHCCCHHCCCCCCC YGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYA CCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC DPNKKVISYWTMGFNQHTRGVWANNLVYNLHLLTGKISQPGCGPFSLTGQPSACGTAREV CCCCCEEEEEECCCCCCCCCEEECCEEEEEEEEECCCCCCCCCCEECCCCCCCCCCHHHH GTFAHRLPADMVVTNEKHRDICEKKWNIPSDTIPAKIGLHAVAQDRALKDGKLNVYWTMC HHHHHHCCCCEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEE TNNMQAGPNINEERMPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAER CCCCCCCCCCCHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEECCCCCCCHHH RTQFWRQQVQAPGEAKSDLWQLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPE HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCC VSKYPVSELAEDQLNDESRELGFYLQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPV CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCC VNGKETQWRYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGR CCCCCCCEEECCCCCCEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCCHHEEECCCH VLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETR HHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCEEEEECCCCEEEEEECC GRNRPPQGLVYMPFFDAAQLVNKLTLDATDPLSKETDFKKCAVKLEKV CCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCC >Mature Secondary Structure MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKAPCRFCGTGCGVLVGTQ CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHCCCCCCHHHHCCCCCEEEECC QGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLLRMKNGKYDKEGEFTPITW CCCEEEECCCCCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCH DQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAASKLFKAGFRSNNIDPNARHCM HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHH ASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVA HHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEE VLSTYQHRSFELADNGIIFTPQSDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIG EEECCCCCCEEECCCCEEECCCCCEEHHHHHHHHHHCCCCCCHHHHHHCCCHHCCCCCCC YGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYA CCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC DPNKKVISYWTMGFNQHTRGVWANNLVYNLHLLTGKISQPGCGPFSLTGQPSACGTAREV CCCCCEEEEEECCCCCCCCCEEECCEEEEEEEEECCCCCCCCCCEECCCCCCCCCCHHHH GTFAHRLPADMVVTNEKHRDICEKKWNIPSDTIPAKIGLHAVAQDRALKDGKLNVYWTMC HHHHHHCCCCEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEE TNNMQAGPNINEERMPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAER CCCCCCCCCCCHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEECCCCCCCHHH RTQFWRQQVQAPGEAKSDLWQLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPE HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCC VSKYPVSELAEDQLNDESRELGFYLQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPV CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCC VNGKETQWRYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGR CCCCCCCEEECCCCCCEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCCHHEEECCCH VLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETR HHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCEEEEECCCCEEEEEECC GRNRPPQGLVYMPFFDAAQLVNKLTLDATDPLSKETDFKKCAVKLEKV CCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA