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The map label for this gene is yphF

Identifier: 157155581

GI number: 157155581

Start: 2828303

End: 2829247

Strand: Reverse

Name: yphF

Synonym: EcE24377A_2833

Alternate gene names: 157155581

Gene position: 2829247-2828303 (Counterclockwise)

Preceding gene: 157155956

Following gene: 157154916

Centisome position: 56.82

GC content: 46.46

Gene sequence:

>945_bases
ATGGCTACGCTATTAGGTAGCGCTTTATTTGCCAGGGCTGCGGAAAAAGAAATGACCATTGGCGCAATATACCTTGATAC
TCAGGGATATTACGCTGGAGTGCGTCAAGGCGTTCAGGATGCGGCAAAAGATTCTTCAGTACAGGTACAGTTAATTGAAA
CTAACGCCCAGGGTGATATTTCGAAAGAAAGTACCTTTGTTGATACCCTCGTGGCGCGTAATGTCGATGCCATTATTTTA
TCGGCAGTGTCTGAAAATGGCAGTAGCCGTACCGTTCGTCGCGCCAGTGAAGCGGGTATTCCGGTGATTTGCTACAACAC
CTGTATTAATCAAAAGGGTGTCGATAAATATGTCTCGGCGTATCTGGTCGGCGATCCACTGGAATTTGGTAAAAAACTGG
GTAACGCTGCCGCCGATTATTTTATTGCCAATAAAATTGACCAGCCGAAAATTGCCGTCATCAATTGCGAAGCCTTTGAA
GTTTGTGTGCAGCGACGTAAAGGATTTGAAGAAGTATTAAAATCCCGCGTTCCCGGCGCGCAAATTGTCGCTAATCAGGA
AGGGACTGTTTTAGATAAAGCGATTTCCGTTGGTGAAAAACTGATTATCTCCACGCCGGATCTCAACGCCATTATGGGGG
AGTCGGGCGGTGCGACACTCGGCGCGGTAAAAGCGGTACGTAATCAAAATCAGGCCGGAAAAATTGCTGTTTTCGGTTCG
GATATGACAACCGAAATTGCTCAGGAGCTGGAAAACAATCAGGTGCTGAAAGCGGTAGTGGATATTTCCGGTAAGAAAAT
GGGCAATGCTGTTTTCGCGCAAACATTGAAGGTTATCAATAAACAAGCCGACGGTGAAAAAGTGATTCAGGTGCCTATCG
ATCTCTATACCAAAACGGAAGACGGTAAACAGTGGCTGGCAACGCACGTTGATGGTCTGCCCTAA

Upstream 100 bases:

>100_bases
GCCGCTCATCACAAAATAATTAGAATAAGCCGTTGTTAATAAAAACCCTACAGGAGGCTCTATGCCTAAAAAAATGAGAA
CAACCCGTAATCTATTATTA

Downstream 100 bases:

>100_bases
TCGCTGATTCGCAGAGGTCCCTATGTTCACGGCAACAGAGGCAGTCCCGGTAGCAAAAGTGGTGGCAGGAAATAAGCGTT
ATCCCGGCGTCGTTGCGTTG

Product: putative sugar ABC transporter, periplasmic sugar-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MATLLGSALFARAAEKEMTIGAIYLDTQGYYAGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIIL
SAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKIAVINCEAFE
VCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGS
DMTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP

Sequences:

>Translated_314_residues
MATLLGSALFARAAEKEMTIGAIYLDTQGYYAGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIIL
SAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKIAVINCEAFE
VCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGS
DMTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP
>Mature_313_residues
ATLLGSALFARAAEKEMTIGAIYLDTQGYYAGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILS
AVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKIAVINCEAFEV
CVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSD
MTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP

Specific function: Probably part of the binding-protein-dependent transport system yphDEF

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family

Homologues:

Organism=Escherichia coli, GI1788898, Length=314, Percent_Identity=100, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI1788473, Length=261, Percent_Identity=26.4367816091954, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YPHF_ECOLI (P77269)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C65032
- RefSeq:   AP_003134.1
- RefSeq:   NP_417043.1
- ProteinModelPortal:   P77269
- SMR:   P77269
- STRING:   P77269
- EnsemblBacteria:   EBESCT00000004684
- EnsemblBacteria:   EBESCT00000015429
- GeneID:   947020
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2532
- KEGG:   eco:b2548
- EchoBASE:   EB3240
- EcoGene:   EG13467
- eggNOG:   COG1879
- GeneTree:   EBGT00050000009242
- HOGENOM:   HBG467181
- OMA:   RANEANI
- ProtClustDB:   CLSK880430
- BioCyc:   EcoCyc:G7342-MONOMER
- Genevestigator:   P77269
- GO:   GO:0006810
- InterPro:   IPR001761

Pfam domain/function: PF00532 Peripla_BP_1

EC number: NA

Molecular weight: Translated: 33502; Mature: 33370

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLLGSALFARAAEKEMTIGAIYLDTQGYYAGVRQGVQDAAKDSSVQVQLIETNAQGDI
CCHHHHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
SKESTFVDTLVARNVDAIILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSA
CCHHHHHHHHHHCCHHHEEEEHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
YLVGDPLEFGKKLGNAAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGA
HHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCHHHHHHHCCCCC
QIVANQEGTVLDKAISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGS
EEEECCCCCHHHHHHHCCCEEEEECCCCHHEECCCCCCHHHHHHHHHCCCCCCEEEEECC
DMTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTE
CHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCC
DGKQWLATHVDGLP
CHHHHHHHHCCCCC
>Mature Secondary Structure 
ATLLGSALFARAAEKEMTIGAIYLDTQGYYAGVRQGVQDAAKDSSVQVQLIETNAQGDI
CHHHHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
SKESTFVDTLVARNVDAIILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSA
CCHHHHHHHHHHCCHHHEEEEHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
YLVGDPLEFGKKLGNAAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGA
HHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCHHHHHHHCCCCC
QIVANQEGTVLDKAISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGS
EEEECCCCCHHHHHHHCCCEEEEECCCCHHEECCCCCCHHHHHHHHHCCCCCCEEEEECC
DMTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTE
CHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCC
DGKQWLATHVDGLP
CHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 9298646