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The map label for this gene is puuP [H]

Identifier: 157155487

GI number: 157155487

Start: 1489529

End: 1490914

Strand: Reverse

Name: puuP [H]

Synonym: EcE24377A_1505

Alternate gene names: 157155487

Gene position: 1490914-1489529 (Counterclockwise)

Preceding gene: 157154986

Following gene: 157157343

Centisome position: 29.94

GC content: 53.54

Gene sequence:

>1386_bases
ATGGCTATTAATTCACCACTGAATATTGCTGCGCAACCCGGCAAAACCCGTCTGCGAAAATCACTGAAATTGTGGCAGGT
GGTAATGATGGGTCTGGCCTATCTCACGCCGATGACCGTATTTGATACTTTTGGTATTGTCTCCGGCATTAGCGACGGTC
ACGTTCCAGCGTCCTATTTGCTGGCGCTGGCGGGCGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTT
CCGGAGGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAATCCGCACGTCGGATTTATGGTCGGCTGGTCATCACT
GCTGGATTATCTCTTTTTGCCAATGATCAACGTTTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGC
CGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTGGTCGCTAACTTCAAT
ACCCTGTTTGTACTGGTGCAAATCTCCATCATGGTGGTGTTTATCTTCCTGGTGGTTCAGGGACTGCATAAAGGAGAAGG
CGTTGGCACCGTCTGGTCACTTCAGCCGTTTATCAGCGAGAACGCGCACCTGATCCCGATTATTACCGGTGCGACGATTG
TCTGTTTCTCGTTCCTCGGTTTCGATGCGGTGACCACGCTTTCGGAAGAGACGCCGGACGCCGCACGCGTGATCCCGAAA
GCCATCTTCCTGACGGCGGTATACGGCGGCGTTATCTTTATCGCGGCGTCGTTCTTTATGCAGCTGTTCTTTCCCGATAT
CAGCCGCTTTAAAGACCCGGACGCCGCACTGCCTGAAATTGCGCTGTACGTCGGCGGTAAGCTGTTCCAGTCGATTTTCC
TTTGCACCACGTTTGTGAACACGTTAGCGTCTGGCCTGGCCTCACATGCCAGCGTGTCACGTCTGCTGTATGTGATGGGG
CGCGACAATGTGTTTCCGGAGCGCTTGTTTGGCTATGTGCATCCGAAATGGCGGACTCCGGCACTGAACGTCATTATGGT
CGGGATTGTCGCGATGTCGGCGCTGTTCTTCGATTTAGTTACCGCCACAGCGTTAATTAACTTCGGCGCGTTGGTAGCGT
TTACCTTCGTTAACCTGTCGGTGTTCAACCACTTCTGGCGGCGCAAAGGAATGAATAAAAGCTGGAAGGAACACTTCCAC
TATTTATTAATGCCGCTGGTTGGCGCGCTGACGGTGGGCGTGCTGTGGATTAACCTCGAAGCCACCTCGCTGACGCTGGG
TCTGGTATGGGCTTCGTTAGGCGGCGCATATTTGTGGTATCTGATCCGCCGCTATCGCAAAGTGCCGCTGTACGAAGGTG
ACAGAACGCCAGTGAGCGAAACGTAA

Upstream 100 bases:

>100_bases
GCTACTGACTGGACTCCGCCCCCTTTGCTTCCCTTGCTAAACAGGTTTGATTTCAGGTCGACACGACCGCAAACCTTATT
ACGCAGGGGAGGCAGCAATT

Downstream 100 bases:

>100_bases
TAGTCGGATGCGCGCCGCATTATCCTGCCTGAGGGTAATGCGCCGCGCCATTAACTATAAAAAGATGTATGACCCGGATC
ACGAGTGCTATCATCCGCCC

Product: amino acid permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQF
PEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMG
RDNVFPERLFGYVHPKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKEHFH
YLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPLYEGDRTPVSET

Sequences:

>Translated_461_residues
MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQF
PEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMG
RDNVFPERLFGYVHPKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKEHFH
YLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPLYEGDRTPVSET
>Mature_460_residues
AINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFP
EAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNT
LFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPKA
IFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGR
DNVFPERLFGYVHPKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKEHFHY
LLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPLYEGDRTPVSET

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI114326544, Length=425, Percent_Identity=22.8235294117647, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI114326550, Length=425, Percent_Identity=22.8235294117647, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4507047, Length=415, Percent_Identity=22.4096385542169, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI87081869, Length=461, Percent_Identity=98.6984815618221, Blast_Score=920, Evalue=0.0,
Organism=Escherichia coli, GI87082023, Length=446, Percent_Identity=63.2286995515695, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1788480, Length=395, Percent_Identity=24.0506329113924, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081708, Length=467, Percent_Identity=24.8394004282655, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786789, Length=175, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786602, Length=176, Percent_Identity=25.5681818181818, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082250, Length=403, Percent_Identity=22.5806451612903, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786302, Length=173, Percent_Identity=26.0115606936416, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=408, Percent_Identity=24.0196078431373, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17533459, Length=404, Percent_Identity=24.5049504950495, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531343, Length=459, Percent_Identity=25.2723311546841, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24667468, Length=406, Percent_Identity=25.8620689655172, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24668806, Length=418, Percent_Identity=25.1196172248804, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21356285, Length=418, Percent_Identity=25.1196172248804, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24668802, Length=418, Percent_Identity=25.1196172248804, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281366235, Length=411, Percent_Identity=24.5742092457421, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI116007820, Length=411, Percent_Identity=24.5742092457421, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI116007818, Length=413, Percent_Identity=24.9394673123487, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI85725152, Length=413, Percent_Identity=24.9394673123487, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI221512776, Length=396, Percent_Identity=20.959595959596, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24666159, Length=396, Percent_Identity=20.959595959596, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI161077963, Length=399, Percent_Identity=22.3057644110276, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50912; Mature: 50781

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFIFLVVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
GLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
HHCCCCCCCEEEECCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLASGLASHASVSRLLYVMGRDNVFPERLFGYVHPKWRTPALNVIMVGIVAMSALFFDLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKEHFHYLLMPLVGALTVGVLWINLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEE
ATSLTLGLVWASLGGAYLWYLIRRYRKVPLYEGDRTPVSET
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFIFLVVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
GLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
HHCCCCCCCEEEECCHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLASGLASHASVSRLLYVMGRDNVFPERLFGYVHPKWRTPALNVIMVGIVAMSALFFDLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKEHFHYLLMPLVGALTVGVLWINLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEE
ATSLTLGLVWASLGGAYLWYLIRRYRKVPLYEGDRTPVSET
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]