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The map label for this gene is rutA

Identifier: 157155454

GI number: 157155454

Start: 1150513

End: 1151694

Strand: Reverse

Name: rutA

Synonym: EcE24377A_1130

Alternate gene names: 157155454

Gene position: 1151694-1150513 (Counterclockwise)

Preceding gene: 157158674

Following gene: 157158711

Centisome position: 23.13

GC content: 54.57

Gene sequence:

>1182_bases
TTGCAAACAAGCCATTACGCGGCTGAAAAGGATATGCAGGACGCAGTGCCCCGTCTGACTTTCACTTTACGAGACGAAGA
GAGGTTGATGATGAAAATTGGCGTATTCGTACCTATTGGCAACAACGGCTGGCTCATTTCGACCCACGCGCCGCAGTACA
TGCCGACCTTTGAACTGAATAAAGCCATTGTACAAAAAGCGGAGCACTACCATTTCGATTTCGCCCTGTCGATGATCAAA
CTGCGTGGCTTTGGCGGCAAAACTGAGTTCTGGGATCACAACCTTGAGTCGTTCACCTTGATGGCGGGGCTGGCAGCCGT
CACCTCACGCATTCAGATTTACGCGACAGCTGCCACCTTAACGTTACCTCCGGCAATCGTCGCCCGTATGGCCGCAACCA
TTGACTCCATCTCTGGCGGGCGTTTTGGCGTCAACCTCGTGACTGGCTGGCAAAAGCCCGAGTATGAGCAGATGGGTATC
TGGCCTGGCGATGACTATTTCTCCCGTCGTTACGACTATCTCACCGAATATGTTCAGGTGCTGCGCGACCTGTGGGGCTC
GGGGAAAAGCGATTTTAAAGGCGATTTTTTCACCATGAATGATTGTCGCGTCAGTCCGCAACCGAGTGTACCCATGAAAG
TGATCTGCGCCGGGCAAAGCGACGCTGGCATGGCGTTCTCCGCCCAGTATGCCGATTTCAACTTCTGTTTCGGCAAAGGC
GTAAATACACCCACGGCTTTCGCCCCGACCGCTGCGCGGATGAAACAGGCCGCAGAGCAAACCGGGCGCGACGTTGGCTC
TTATGTATTGTTTATGGTGATTGCCGATGAAACCGACGATGCCGCTCGCGCCAAATGGGAACACTACAAAGCGGGCGCGG
ATGAAGAGGCGTTAAGCTGGCTAACCGAACAAAGTCAGAAAGATACCCGCTCCGGTACTGACACCAACGTCCGTCAGATG
GCCGATCCCACTTCGGCGGTAAACATCAATATGGGGACGTTAGTCGGTTCTTACGCCAGTGTCGCGCGCATGTTAGATGA
AGTCGCAAGCGTGCCTGGTGCCGAAGGCGTGCTGTTGACCTTCGATGATTTTCTGTCGGGAATCGAAACCTTCGGCGAGC
GCATTCAACCACTGATGCAGTGCCGCGCCCATCTCCCTGCGCTGACTCAGGAGGTGGCATGA

Upstream 100 bases:

>100_bases
TGCAACTGTTTTGACCGTTTAGTCCACTTTTTACCAGATATTTTAGATAACTTAGCATTAACACATTAATAACATTCACT
TTTAAAAACTGGCATCCACT

Downstream 100 bases:

>100_bases
TGACGACCTTAACCGCTCGACCGGAAGCCATTACCTTCGATCCGCAGCAAAGTGCGCTGATCGTGGTGGATATGCAAAAC
GCTTATGCCACGCCAGGAGG

Product: putative monooxygenase rutA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MQTSHYAAEKDMQDAVPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIK
LRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGI
WPGDDYFSRRYDYLTEYVQVLRDLWGSGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQM
ADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA

Sequences:

>Translated_393_residues
MQTSHYAAEKDMQDAVPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIK
LRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGI
WPGDDYFSRRYDYLTEYVQVLRDLWGSGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQM
ADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA
>Mature_393_residues
MQTSHYAAEKDMQDAVPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIK
LRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGI
WPGDDYFSRRYDYLTEYVQVLRDLWGSGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQM
ADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA

Specific function: Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product wh

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family. RutA subfamily

Homologues:

Organism=Escherichia coli, GI1787247, Length=382, Percent_Identity=99.2146596858639, Blast_Score=794, Evalue=0.0,
Organism=Escherichia coli, GI1787166, Length=305, Percent_Identity=24.5901639344262, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTA_ECO24 (A7ZKB7)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462246.1
- STRING:   A7ZKB7
- EnsemblBacteria:   EBESCT00000023175
- GeneID:   5589956
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_1130
- NMPDR:   fig|331111.3.peg.3613
- eggNOG:   COG2141
- GeneTree:   EBGT00050000008394
- HOGENOM:   HBG350183
- OMA:   FIPIGNN
- ProtClustDB:   CLSK879925
- BioCyc:   ECOL331111:ECE24377A_1130-MONOMER
- HAMAP:   MF_01699
- InterPro:   IPR011251
- InterPro:   IPR019914
- InterPro:   IPR016048
- Gene3D:   G3DSA:3.20.20.30
- TIGRFAMs:   TIGR03612

Pfam domain/function: PF00296 Bac_luciferase; SSF51679 Luciferase_like

EC number: NA

Molecular weight: Translated: 43468; Mature: 43468

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: BINDING 145-145 BINDING 154-154 BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSHYAAEKDMQDAVPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELN
CCCCCHHHHHHHHHCCCCEEEEECCHHHEEEEEEEEEEECCCCEEEECCCCCCCCCHHHH
KAIVQKAEHYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEE
TLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQV
ECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LRDLWGSGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
HHHHHCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEECCC
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSW
CCCCCCCCCHHHHHHHHHHHHCCHHHCEEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHH
LTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLT
HHHHHHHHCCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
FDDFLSGIETFGERIQPLMQCRAHLPALTQEVA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure
MQTSHYAAEKDMQDAVPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELN
CCCCCHHHHHHHHHCCCCEEEEECCHHHEEEEEEEEEEECCCCEEEECCCCCCCCCHHHH
KAIVQKAEHYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEE
TLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQV
ECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHH
LRDLWGSGKSDFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKG
HHHHHCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEECCC
VNTPTAFAPTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSW
CCCCCCCCCHHHHHHHHHHHHCCHHHCEEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHH
LTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLT
HHHHHHHHCCCCCCCCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
FDDFLSGIETFGERIQPLMQCRAHLPALTQEVA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA