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The map label for this gene is intA [H]

Identifier: 157155411

GI number: 157155411

Start: 319243

End: 320565

Strand: Reverse

Name: intA [H]

Synonym: EcE24377A_0292

Alternate gene names: 157155411

Gene position: 320565-319243 (Counterclockwise)

Preceding gene: 157154740

Following gene: 157155484

Centisome position: 6.44

GC content: 49.89

Gene sequence:

>1323_bases
ATGGCAACAGTGATAACTCAGGATCGACAGATAGCAAGCCTTGTTCCCCCTGTAGGAAAAAACCGGATTGTCGTCAGCGT
CAAATCAAAAGCAGGTGCGGGGTTGTATATCGAATCCCGATCAGGGAGCCAAACAAAGAGTTGGCTCTACCGTCCCTACC
TCAACGGCAAGCAGATAAAAATTACCCTGGGAGCTTATCCTGCAATGACGCTGGCCCAGGCTCGTGAAGCACATGCTGAA
GCCATTGAGTTAGTGAAGCAGGGAATTGACCCCAGATACGTTCGTAAAACTGAAAAATCGAATAATGAGCAGATGCCCAT
ATTTTCTGAATTGTGGGAAAACTGGTTGTCATTCAGAGAAACCAGCAAGCCAATAGGGGCTCGTACACTGGCTGATTATA
AGGGAACGTATCGTCGCCATCTTGAGCAGGGGCTAGGCTCCGTGAGAGTGTGCGATTTATCGCGTTCGTATTTGTTTGCA
CACCTGAGCAAAGTCCGACAATCCAGCGCTGAAGGTGTGCGCAAAGGGCTTATCATCCTGAATATGACACTTGACCATGC
CACACTTCAGGGATTAATCGAGCTCAACCCTGCCCGTCTGTTAAAACCGGCGATGTTTGGTGCTTCCATGGCTAAACCGC
GTGAGCGCTGGTTATCGCAGGATGAGCTTCAACGGCTCTGGAAAACACTAGAAGAAGCTACTGCTGGTGGCGGTTCAGTT
AGCACCGGTGGAAAAGGTATCGCTTCCAGTGTCGTTTTATCCCATGCGATAGCTAACACACTGAAGCTGATTATTCTGAC
CGGCGTTCGCCGCTCTGAAGCGGTTAAAATGCGCTGGGATCAAATCAACGGCGATCGCTGGACAATACCGGAAACAAAGA
ATGGTAAAAGTCATGTAGTAACACTGCATCCGCTGGCACTGTCTATCCTGAAGAAACAGCGAATTATCTCCGAAGGCGCA
TTTGTCTTTGAGTCCACCAGCAACCAGGGATTCCCTATAACCGGAGACGCTGTCACACGAGCACTGGAGCGCCTGCGTAA
AAAATATCTGGCAGAGCTGGAGCCATTTTCTCCCCATGATTTACGCCGCAGTGTCGCTACTGGTTGCGCTGAATATCTGG
ATGCCCCGGAGCGCCTGATTGAGCGATTGTTAAACCACATTCCTAAAGACCGCCTGATCAGAACCTATCAGGTCGGGCAG
CAGGCGGAAAAATTGCGTAGTTTGTTTTTGAGCTGGGGGGATTTTGTGGAGCGGTATGTAGCGCAGTCTGCCAGCCATGA
GGTGACGGATAATGTTGTTCAGGTTAAGTTTGGCGGCAGATAA

Upstream 100 bases:

>100_bases
TCAATAAATTAAGACAAAAACACCTTTCACGTTGTTTCATGCCATTTCGCCTTATACAATGCTAAACAAGTACGCCAAAA
AGTACGCGGGATGAGAAAAA

Downstream 100 bases:

>100_bases
CGTAATACTCGCTCTGATGGCGTTCAATGAACAAAAAACTCTCATATCAGTCGGTCAACTCGATACTAATAGTGGGTATC
AGCGATAGATTTCATGGTAA

Product: phage integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MATVITQDRQIASLVPPVGKNRIVVSVKSKAGAGLYIESRSGSQTKSWLYRPYLNGKQIKITLGAYPAMTLAQAREAHAE
AIELVKQGIDPRYVRKTEKSNNEQMPIFSELWENWLSFRETSKPIGARTLADYKGTYRRHLEQGLGSVRVCDLSRSYLFA
HLSKVRQSSAEGVRKGLIILNMTLDHATLQGLIELNPARLLKPAMFGASMAKPRERWLSQDELQRLWKTLEEATAGGGSV
STGGKGIASSVVLSHAIANTLKLIILTGVRRSEAVKMRWDQINGDRWTIPETKNGKSHVVTLHPLALSILKKQRIISEGA
FVFESTSNQGFPITGDAVTRALERLRKKYLAELEPFSPHDLRRSVATGCAEYLDAPERLIERLLNHIPKDRLIRTYQVGQ
QAEKLRSLFLSWGDFVERYVAQSASHEVTDNVVQVKFGGR

Sequences:

>Translated_440_residues
MATVITQDRQIASLVPPVGKNRIVVSVKSKAGAGLYIESRSGSQTKSWLYRPYLNGKQIKITLGAYPAMTLAQAREAHAE
AIELVKQGIDPRYVRKTEKSNNEQMPIFSELWENWLSFRETSKPIGARTLADYKGTYRRHLEQGLGSVRVCDLSRSYLFA
HLSKVRQSSAEGVRKGLIILNMTLDHATLQGLIELNPARLLKPAMFGASMAKPRERWLSQDELQRLWKTLEEATAGGGSV
STGGKGIASSVVLSHAIANTLKLIILTGVRRSEAVKMRWDQINGDRWTIPETKNGKSHVVTLHPLALSILKKQRIISEGA
FVFESTSNQGFPITGDAVTRALERLRKKYLAELEPFSPHDLRRSVATGCAEYLDAPERLIERLLNHIPKDRLIRTYQVGQ
QAEKLRSLFLSWGDFVERYVAQSASHEVTDNVVQVKFGGR
>Mature_439_residues
ATVITQDRQIASLVPPVGKNRIVVSVKSKAGAGLYIESRSGSQTKSWLYRPYLNGKQIKITLGAYPAMTLAQAREAHAEA
IELVKQGIDPRYVRKTEKSNNEQMPIFSELWENWLSFRETSKPIGARTLADYKGTYRRHLEQGLGSVRVCDLSRSYLFAH
LSKVRQSSAEGVRKGLIILNMTLDHATLQGLIELNPARLLKPAMFGASMAKPRERWLSQDELQRLWKTLEEATAGGGSVS
TGGKGIASSVVLSHAIANTLKLIILTGVRRSEAVKMRWDQINGDRWTIPETKNGKSHVVTLHPLALSILKKQRIISEGAF
VFESTSNQGFPITGDAVTRALERLRKKYLAELEPFSPHDLRRSVATGCAEYLDAPERLIERLLNHIPKDRLIRTYQVGQQ
AEKLRSLFLSWGDFVERYVAQSASHEVTDNVVQVKFGGR

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=403, Percent_Identity=27.0471464019851, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI145693166, Length=390, Percent_Identity=26.6666666666667, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788690, Length=396, Percent_Identity=24.7474747474747, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 49212; Mature: 49081

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVITQDRQIASLVPPVGKNRIVVSVKSKAGAGLYIESRSGSQTKSWLYRPYLNGKQIK
CCCEECCCCHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHCCCCCCCCEEE
ITLGAYPAMTLAQAREAHAEAIELVKQGIDPRYVRKTEKSNNEQMPIFSELWENWLSFRE
EEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
TSKPIGARTLADYKGTYRRHLEQGLGSVRVCDLSRSYLFAHLSKVRQSSAEGVRKGLIIL
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
NMTLDHATLQGLIELNPARLLKPAMFGASMAKPRERWLSQDELQRLWKTLEEATAGGGSV
EEEHHHHHHHHHHHCCHHHHHHHHHHCCHHCCHHHHHCCHHHHHHHHHHHHHHHCCCCCC
STGGKGIASSVVLSHAIANTLKLIILTGVRRSEAVKMRWDQINGDRWTIPETKNGKSHVV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEEE
TLHPLALSILKKQRIISEGAFVFESTSNQGFPITGDAVTRALERLRKKYLAELEPFSPHD
EEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
LRRSVATGCAEYLDAPERLIERLLNHIPKDRLIRTYQVGQQAEKLRSLFLSWGDFVERYV
HHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQSASHEVTDNVVQVKFGGR
HHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
ATVITQDRQIASLVPPVGKNRIVVSVKSKAGAGLYIESRSGSQTKSWLYRPYLNGKQIK
CCEECCCCHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHCCCCCCCCEEE
ITLGAYPAMTLAQAREAHAEAIELVKQGIDPRYVRKTEKSNNEQMPIFSELWENWLSFRE
EEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
TSKPIGARTLADYKGTYRRHLEQGLGSVRVCDLSRSYLFAHLSKVRQSSAEGVRKGLIIL
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
NMTLDHATLQGLIELNPARLLKPAMFGASMAKPRERWLSQDELQRLWKTLEEATAGGGSV
EEEHHHHHHHHHHHCCHHHHHHHHHHCCHHCCHHHHHCCHHHHHHHHHHHHHHHCCCCCC
STGGKGIASSVVLSHAIANTLKLIILTGVRRSEAVKMRWDQINGDRWTIPETKNGKSHVV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEEE
TLHPLALSILKKQRIISEGAFVFESTSNQGFPITGDAVTRALERLRKKYLAELEPFSPHD
EEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
LRRSVATGCAEYLDAPERLIERLLNHIPKDRLIRTYQVGQQAEKLRSLFLSWGDFVERYV
HHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQSASHEVTDNVVQVKFGGR
HHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]