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The map label for this gene is gutB [H]

Identifier: 157155393

GI number: 157155393

Start: 1983412

End: 1984455

Strand: Reverse

Name: gutB [H]

Synonym: EcE24377A_1998

Alternate gene names: 157155393

Gene position: 1984455-1983412 (Counterclockwise)

Preceding gene: 157155942

Following gene: 157154881

Centisome position: 39.85

GC content: 46.36

Gene sequence:

>1044_bases
ATGAAAAATTCAAAAGCAATATTGCAGGTGCCGGGCACAATGAAAATTATTTCAGCAGAAATACCAGTGCCTAAAGAAGA
TGAAGTTTTGATTAAAGTAGAATATGTCGGTATTTGTGGTTCAGATGTACATGGTTTTGAATCAGGCCCGTTTATTCCGC
CTAAAGACCCAAATCAAGAAATTGGCCTGGGTCATGAATGCGCCGGGACGGTTGTGGCTGTGGGAAGCCGCGTGCGCAAA
TTTAAACCGGGGGATCGGGTAAATATCGAACCTGGCGTTCCTTGCGGTCACTGTCGTTACTGTCTGGAAGGCAAATATAA
CATCTGCCCGGACGTTGATTTTATGGCGACACAACCCAACTACCGCGGCGCATTAACGCACTATCTGTGTCATCCGGAGA
GCTTTACTTACAAACTGCCAGACAATATGGACACGATGGAAGGGGCGCTGGTGGAGCCTGCCGCAGTCGGGATGCATGCC
GCGATGCTGGCAGATGTTAAACCGGGTAAGAAGATAATTATTCTGGGAGCAGGTTGTATTGGTTTGATGACGTTGCAAGC
GTGCAAATGCCTGGGCGCAACGGAAATTGCCGTCGTTGATGTGCTGGAAAAACGTCTGATAATGGCGGAACAACTTGGTG
CGACAGTGGTTATTAACGGCACAAAAGAAGACACTATTGCACGCTGTCAGCAATTTACCGAAGACATGGGCGCAGATATT
GTTTTCGAAACAGCGGGTTCTGCGGTCACCGTTAAACAGGCACCTTATCTGGTAATGCGCGGCGGTAAAATTATGATTGT
TGGTACTGTACCCGGCGATTCGGCAATCAATTTCCTCAAAATCAATCGCGAAGTCACTATCCAGACGGTATTCCGCTATG
CCAATCGTTATCCGGTCACGATTGAAGCTATTTCTTCAGGGCGATTCGATGTGAAATCGATGGTGACGCATATTTACGAT
TATCGGGATGTACAACAGGCATTTGAAGAGTCAGTTAACAACAAACGCGACATTATTAAAGGCGTTATTAAAATTAGCGA
TTAA

Upstream 100 bases:

>100_bases
GCAATTGCTATCGCCACCATTGGAATTGAAACCAAAGGTGTCTCCGTTGAAAGTTTAAGTATTGATGCAGTAGCCAATAA
ATAATACGGGTGGATTCAAA

Downstream 100 bases:

>100_bases
TTAATACGGGAGAACAGAACATGCTCGCAGATATCAGATATTGGGAAAACGATGCCACAAATAAACATTATGCAATTGCC
CATTTTAACGTATGGAATGC

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRK
FKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVINGTKEDTIARCQQFTEDMGADI
VFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAINFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYD
YRDVQQAFEESVNNKRDIIKGVIKISD

Sequences:

>Translated_347_residues
MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRK
FKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVINGTKEDTIARCQQFTEDMGADI
VFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAINFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYD
YRDVQQAFEESVNNKRDIIKGVIKISD
>Mature_347_residues
MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRK
FKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVINGTKEDTIARCQQFTEDMGADI
VFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAINFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYD
YRDVQQAFEESVNNKRDIIKGVIKISD

Specific function: Unknown

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=333, Percent_Identity=38.7387387387387, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI4501929, Length=355, Percent_Identity=26.1971830985915, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI34577061, Length=354, Percent_Identity=25.9887005649718, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI4501933, Length=358, Percent_Identity=26.536312849162, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=27.4238227146814, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI156523966, Length=361, Percent_Identity=27.4238227146814, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI71743840, Length=340, Percent_Identity=25.8823529411765, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI262073058, Length=340, Percent_Identity=25.8823529411765, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI71565154, Length=366, Percent_Identity=25.4098360655738, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI194239674, Length=252, Percent_Identity=25.3968253968254, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI13236495, Length=252, Percent_Identity=25.3968253968254, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788073, Length=347, Percent_Identity=99.4236311239193, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI1790045, Length=329, Percent_Identity=29.1793313069909, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1788407, Length=335, Percent_Identity=29.2537313432836, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1788075, Length=339, Percent_Identity=28.3185840707965, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787863, Length=345, Percent_Identity=26.9565217391304, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI226510992, Length=343, Percent_Identity=28.5714285714286, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790718, Length=308, Percent_Identity=29.5454545454545, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87082125, Length=268, Percent_Identity=30.9701492537313, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786552, Length=367, Percent_Identity=28.0653950953678, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081918, Length=259, Percent_Identity=30.1158301158301, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17562876, Length=349, Percent_Identity=36.1031518624642, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17562878, Length=344, Percent_Identity=36.6279069767442, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI71988145, Length=260, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17562584, Length=273, Percent_Identity=29.3040293040293, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25146526, Length=368, Percent_Identity=23.0978260869565, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17562582, Length=274, Percent_Identity=25.9124087591241, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323099, Length=350, Percent_Identity=36.5714285714286, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6322619, Length=352, Percent_Identity=34.375, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319955, Length=352, Percent_Identity=34.375, Blast_Score=193, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6319257, Length=353, Percent_Identity=30.028328611898, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319258, Length=361, Percent_Identity=27.7008310249307, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320033, Length=366, Percent_Identity=27.3224043715847, Blast_Score=105, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6319621, Length=334, Percent_Identity=28.4431137724551, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323729, Length=298, Percent_Identity=27.1812080536913, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=289, Percent_Identity=27.3356401384083, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=309, Percent_Identity=27.1844660194175, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137530, Length=353, Percent_Identity=39.943342776204, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI17737897, Length=353, Percent_Identity=38.2436260623229, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI221457811, Length=326, Percent_Identity=26.6871165644172, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI45550770, Length=326, Percent_Identity=26.6871165644172, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI45551930, Length=326, Percent_Identity=26.6871165644172, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: NA

Molecular weight: Translated: 37773; Mature: 37773

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00059 ADH_ZINC ; PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESGPFIPPKDPNQE
CCCCCEEEECCCCEEEEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
IGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMATQPN
CCCCCHHCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHEECCCCCCCCCCCEEECCCC
YRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHAAMLADVKPGKKIIILGAGCI
CCCCCEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCEEEEEECCHH
GLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVINGTKEDTIARCQQFTEDMGADI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCE
VFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAINFLKINREVTIQTVFRYANRYPVT
EEECCCCEEEEECCCEEEEECCEEEEEEECCCCCCEEEEEECCEEEHHHHHHHHCCCCEE
IEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNNKRDIIKGVIKISD
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECC
>Mature Secondary Structure
MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVHGFESGPFIPPKDPNQE
CCCCCEEEECCCCEEEEEECCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
IGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCLEGKYNICPDVDFMATQPN
CCCCCHHCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHEECCCCCCCCCCCEEECCCC
YRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHAAMLADVKPGKKIIILGAGCI
CCCCCEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCEEEEEECCHH
GLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVINGTKEDTIARCQQFTEDMGADI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCE
VFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAINFLKINREVTIQTVFRYANRYPVT
EEECCCCEEEEECCCEEEEECCEEEEEEECCCCCCEEEEEECCEEEHHHHHHHHCCCCEE
IEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNNKRDIIKGVIKISD
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]