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The map label for this gene is hycB [H]

Identifier: 157155252

GI number: 157155252

Start: 3012429

End: 3013040

Strand: Reverse

Name: hycB [H]

Synonym: EcE24377A_3012

Alternate gene names: 157155252

Gene position: 3013040-3012429 (Counterclockwise)

Preceding gene: 157156901

Following gene: 157155780

Centisome position: 60.51

GC content: 56.54

Gene sequence:

>612_bases
GTGAATCGTTTTGTAATTGCTGACTCCACGCTCTGTATCGGCTGCCACACTTGTGAGGCCGCCTGTTCAGAGACGCATCG
CCAGCACGGCCTGCAATCAATGTCGCGCCTGAGAGTGATGCTAAATGAAAAAGAATCTGCGCCGCAGCTCTGTCACCACT
GTGAAGATGCACCCTGCGCGGTGGTCTGCCCGGTTAACGCCATCACCCGCGTTGATGGGGCCGTGCAGTTGAATGAAAGC
CTGTGCGTAAGCTGCAAGCTGTGCGGCATCGCCTGCCCGTTTGGCGCAATTGAATTTTCCGGCAGCCGTCCGCTGGATAT
TCCTGCAAATGCCAATACCCCGAAAGCGCCACCGGCACCGCCTGCTCCGGCGCGTATCAGTACGCTGCTTGACTGGGTGC
CGGGTATTCGCGCGATCGCCGTCAAATGTGACCTTTGCAGTTTTGATGAACAAGGTCCGGCCTGCGTGCGGATGTGCCCG
ACCAAAGCCCTGCATCTGGTGGATAACACCGATATCGCCCGCGTCAGCAAACGTAAGCGTGAGCTGACTTTTAATACGGA
CTTTGGCGATCTCACCTTGTTTCAGCAGGCTCAAAGTGGAGAGGCTAAATGA

Upstream 100 bases:

>100_bases
GCGTTACGCCTGTTTGGGGATGGGCGTGTCGATGAGTGTCGAAAATGACATTTCATCGGCATGTTTTCGTCAAAAATGAC
AATCACCTGAGGAATGCCTG

Downstream 100 bases:

>100_bases
GCGCAATTTCCCTGATCAATAGCGGCGTGGCGTGGTTTATTGCCGCCGCTGTTCTGGCATTTCTCTTTTCTTTTCAAAAG
ACGCTAAGTGGCTGGATAGC

Product: formate hydrogenlyase, subunit B

Products: Oxidized ferredoxin; CO2; H2 [C]

Alternate protein names: FHL subunit 2; Hydrogenase-3 component B [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMSRLRVMLNEKESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK

Sequences:

>Translated_203_residues
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMSRLRVMLNEKESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK
>Mature_203_residues
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMSRLRVMLNEKESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789079, Length=203, Percent_Identity=99.0147783251232, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI87082114, Length=186, Percent_Identity=50.5376344086022, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI2367245, Length=187, Percent_Identity=39.572192513369, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788811, Length=185, Percent_Identity=38.3783783783784, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1789067, Length=190, Percent_Identity=38.9473684210526, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI87082180, Length=189, Percent_Identity=33.8624338624339, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI87082179, Length=184, Percent_Identity=35.8695652173913, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI2367345, Length=204, Percent_Identity=26.9607843137255, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI226510944, Length=98, Percent_Identity=37.7551020408163, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 21877; Mature: 21877

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
9.9 %Cys+Met (Translated Protein)
7.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
9.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMSRLRVMLNEKESAPQLCHHCEDAPCA
CCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEE
VVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGSRPLDIPANANTPKAPPAP
EEECCHHHHHCCCCEEECCCCEEEEEECEEECCCCCEEECCCCCEECCCCCCCCCCCCCC
PAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCPTKALHLVDNTDIARVSKRKR
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHH
ELTFNTDFGDLTLFQQAQSGEAK
EEEEECCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMSRLRVMLNEKESAPQLCHHCEDAPCA
CCEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEE
VVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGSRPLDIPANANTPKAPPAP
EEECCHHHHHCCCCEEECCCCEEEEEECEEECCCCCEEECCCCCEECCCCCCCCCCCCCC
PAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCPTKALHLVDNTDIARVSKRKR
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHH
ELTFNTDFGDLTLFQQAQSGEAK
EEEEECCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; H+; formate [C]

Specific reaction: 2 reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 formate = CO2 + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]