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The map label for this gene is yeaI [H]

Identifier: 157155149

GI number: 157155149

Start: 853533

End: 854864

Strand: Reverse

Name: yeaI [H]

Synonym: EcE24377A_0835

Alternate gene names: 157155149

Gene position: 854864-853533 (Counterclockwise)

Preceding gene: 157156918

Following gene: 157155693

Centisome position: 17.17

GC content: 29.73

Gene sequence:

>1332_bases
ATGATTATCAACCAGGTACCTATAAAAATAAAAATCTTCATCTTTTTATTTTCATGCATCTCTATTATATTTTTGTTACT
GCATGAAAATAATGGAATATACATAACACAAACAACACAAATAAGTTATAGTGTTTTCATTATTGGGCTTTTTTTCATAA
ACCTGATGATTTTTATTTTTCTATTGCTTTACTATGTTTCTAATCAGAGACAAAGTTATCTCTTAATTCTTTCATTCGCG
TTTTTGAGCAACACGTATTATTTATTAGAAGTGGCTATTATTTCTTTATCTCCGTTAGGTAACGATTTATCTACAATCTA
TCAGAAATCAAATGATATCGCAATATATTATCTATTCCGTCAGTTCAGCTTTATATCTATAATCTTTCTGGCTGTTTATT
CCACCAATGTTAAAAATAAAAGTGTTTTAGAAGATAAAAGAAACATAATAATTGTTGTTTTGTCAATATTAATTCTTTTT
ATTACTCCGTATGTAGCAAAAAATCTAAGCAGTGACAATATAAAATATAGTCTTAATATTATACAATACTCGCTGAATCG
TCATTTGCCGACGTGGAATATCGTGTACACCAAAATAATATCAGTATTTTGGCTTGTATTACTTATCAGCTCATGCATCA
GCATACGTAATTACTCAAAAATATGGTTGTGTATAATACTTATTAGTATAGTGTCAGTATGCAATAATCTAATTTTATTG
TATTTTATTGATAAATCCCATCCTGCATGGTACATGACAAAATTTCTTGAATTGATATCAATGATTTATATCATTTCAAC
ACTCATGTATTATGTTTTCAGGAAATTAAATCATGCTAATCATATGGCAATTCATGATCCACTAACGAATACATACAATA
GAAGATACTTTATTGACTCATTGAAGAATATATCAAAACACCATGATTTCTCAGTAATAATGTTAGATATTGACAGTTTC
AAAAGCATCAATGACAAATGGGGGCATCATATGGGTGATCAAGTCATAGTAATGGTTACCAGAATAATAAAAAAATCCAT
CAGGAAAGAGGATATATTAGGGCGCTTAGGCGGTGAGGAGTTCGGTATTATCATTAAAGGTAATACTCAAAAGCTCTTGC
TATCAATTGCAGAGCGAATCAGAAAAAACATTGAAGAGCAATGCTCGGAAAAATTATTATCGCATGGACCTGAGAAAATA
ACTGTCAGTATTGGTTGCTTTACTTCAAAAGAGAATAATCTCAGCCCATCTGAAATGTTAGTCAATGCCGATAAAGCGTT
ATATCAAGCCAAAAGAACCGGAAAAAACAAGGTGATAATTCACTCAAAATAA

Upstream 100 bases:

>100_bases
TGACAGCGATTAACCAGAATAATAGTATACTATCACCAGTAAGAAATTATCGTTATTTGTAGCGATACATATTATATATA
TATCATTCTCAGGTGCGTAC

Downstream 100 bases:

>100_bases
ACACCTTTTTAAAATACAACCCCAATAAACTGCAGAATATTATCCCATATAATATCCTGCAGTTCGTAATGCACTATTCG
ATAATGGGTACTGTTGGCCA

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MIINQVPIKIKIFIFLFSCISIIFLLLHENNGIYITQTTQISYSVFIIGLFFINLMIFIFLLLYYVSNQRQSYLLILSFA
FLSNTYYLLEVAIISLSPLGNDLSTIYQKSNDIAIYYLFRQFSFISIIFLAVYSTNVKNKSVLEDKRNIIIVVLSILILF
ITPYVAKNLSSDNIKYSLNIIQYSLNRHLPTWNIVYTKIISVFWLVLLISSCISIRNYSKIWLCIILISIVSVCNNLILL
YFIDKSHPAWYMTKFLELISMIYIISTLMYYVFRKLNHANHMAIHDPLTNTYNRRYFIDSLKNISKHHDFSVIMLDIDSF
KSINDKWGHHMGDQVIVMVTRIIKKSIRKEDILGRLGGEEFGIIIKGNTQKLLLSIAERIRKNIEEQCSEKLLSHGPEKI
TVSIGCFTSKENNLSPSEMLVNADKALYQAKRTGKNKVIIHSK

Sequences:

>Translated_443_residues
MIINQVPIKIKIFIFLFSCISIIFLLLHENNGIYITQTTQISYSVFIIGLFFINLMIFIFLLLYYVSNQRQSYLLILSFA
FLSNTYYLLEVAIISLSPLGNDLSTIYQKSNDIAIYYLFRQFSFISIIFLAVYSTNVKNKSVLEDKRNIIIVVLSILILF
ITPYVAKNLSSDNIKYSLNIIQYSLNRHLPTWNIVYTKIISVFWLVLLISSCISIRNYSKIWLCIILISIVSVCNNLILL
YFIDKSHPAWYMTKFLELISMIYIISTLMYYVFRKLNHANHMAIHDPLTNTYNRRYFIDSLKNISKHHDFSVIMLDIDSF
KSINDKWGHHMGDQVIVMVTRIIKKSIRKEDILGRLGGEEFGIIIKGNTQKLLLSIAERIRKNIEEQCSEKLLSHGPEKI
TVSIGCFTSKENNLSPSEMLVNADKALYQAKRTGKNKVIIHSK
>Mature_443_residues
MIINQVPIKIKIFIFLFSCISIIFLLLHENNGIYITQTTQISYSVFIIGLFFINLMIFIFLLLYYVSNQRQSYLLILSFA
FLSNTYYLLEVAIISLSPLGNDLSTIYQKSNDIAIYYLFRQFSFISIIFLAVYSTNVKNKSVLEDKRNIIIVVLSILILF
ITPYVAKNLSSDNIKYSLNIIQYSLNRHLPTWNIVYTKIISVFWLVLLISSCISIRNYSKIWLCIILISIVSVCNNLILL
YFIDKSHPAWYMTKFLELISMIYIISTLMYYVFRKLNHANHMAIHDPLTNTYNRRYFIDSLKNISKHHDFSVIMLDIDSF
KSINDKWGHHMGDQVIVMVTRIIKKSIRKEDILGRLGGEEFGIIIKGNTQKLLLSIAERIRKNIEEQCSEKLLSHGPEKI
TVSIGCFTSKENNLSPSEMLVNADKALYQAKRTGKNKVIIHSK

Specific function: Unknown

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1788085, Length=426, Percent_Identity=38.4976525821596, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1787262, Length=408, Percent_Identity=37.2549019607843, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI87082007, Length=197, Percent_Identity=34.010152284264, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=33.1360946745562, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=35.672514619883, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787802, Length=164, Percent_Identity=35.3658536585366, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693134, Length=189, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081974, Length=182, Percent_Identity=34.0659340659341, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788381, Length=176, Percent_Identity=27.8409090909091, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787056, Length=167, Percent_Identity=31.7365269461078, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=28.4023668639053, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=27.2151898734177, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=155, Percent_Identity=29.0322580645161, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788956, Length=170, Percent_Identity=30.5882352941176, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 51424; Mature: 51424

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINQVPIKIKIFIFLFSCISIIFLLLHENNGIYITQTTQISYSVFIIGLFFINLMIFIF
CCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
LLLYYVSNQRQSYLLILSFAFLSNTYYLLEVAIISLSPLGNDLSTIYQKSNDIAIYYLFR
HHHHHHCCCCCCEEHEEEHHHHCCCHHEEHEEHHHCCCCCCHHHHHHHCCCCEEEEHHHH
QFSFISIIFLAVYSTNVKNKSVLEDKRNIIIVVLSILILFITPYVAKNLSSDNIKYSLNI
HHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
IQYSLNRHLPTWNIVYTKIISVFWLVLLISSCISIRNYSKIWLCIILISIVSVCNNLILL
EEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEE
YFIDKSHPAWYMTKFLELISMIYIISTLMYYVFRKLNHANHMAIHDPLTNTYNRRYFIDS
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHH
LKNISKHHDFSVIMLDIDSFKSINDKWGHHMGDQVIVMVTRIIKKSIRKEDILGRLGGEE
HHHHHHCCCCEEEEEECHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
FGIIIKGNTQKLLLSIAERIRKNIEEQCSEKLLSHGPEKITVSIGCFTSKENNLSPSEML
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHHHHH
VNADKALYQAKRTGKNKVIIHSK
HHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure
MIINQVPIKIKIFIFLFSCISIIFLLLHENNGIYITQTTQISYSVFIIGLFFINLMIFIF
CCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
LLLYYVSNQRQSYLLILSFAFLSNTYYLLEVAIISLSPLGNDLSTIYQKSNDIAIYYLFR
HHHHHHCCCCCCEEHEEEHHHHCCCHHEEHEEHHHCCCCCCHHHHHHHCCCCEEEEHHHH
QFSFISIIFLAVYSTNVKNKSVLEDKRNIIIVVLSILILFITPYVAKNLSSDNIKYSLNI
HHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
IQYSLNRHLPTWNIVYTKIISVFWLVLLISSCISIRNYSKIWLCIILISIVSVCNNLILL
EEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEE
YFIDKSHPAWYMTKFLELISMIYIISTLMYYVFRKLNHANHMAIHDPLTNTYNRRYFIDS
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHH
LKNISKHHDFSVIMLDIDSFKSINDKWGHHMGDQVIVMVTRIIKKSIRKEDILGRLGGEE
HHHHHHCCCCEEEEEECHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
FGIIIKGNTQKLLLSIAERIRKNIEEQCSEKLLSHGPEKITVSIGCFTSKENNLSPSEML
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCHHHHH
VNADKALYQAKRTGKNKVIIHSK
HHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]