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The map label for this gene is yfiE [H]

Identifier: 157155147

GI number: 157155147

Start: 2864065

End: 2864946

Strand: Reverse

Name: yfiE [H]

Synonym: EcE24377A_2864

Alternate gene names: 157155147

Gene position: 2864946-2864065 (Counterclockwise)

Preceding gene: 157159266

Following gene: 157158975

Centisome position: 57.53

GC content: 49.43

Gene sequence:

>882_bases
ATGGATCTGCGCCGTTTTATTACACTTAAAACCGTGGTGGAAGAGGGTTCCTTTTTGCGAGCTTCGCAAAAATTGTGCTG
TACACAATCGACGGTGACTTTTCATATTCAGCAGCTTGAGCAGGAGTTCTCGGTACAATTGTTTGAGAAAATTGGTCGAC
GAATGTGCCTTACCCGCGAAGGAAAAAAGTTGCTGCCGCACATCTATGAACTTACCCGAGTGATGGATACGCTTCGTGAG
GCTGCGAAAAAAGAGTCGGATCCGGACGGGGAACTACGCGTTGTTTCAGGCGAAACGTTGCTCTCTTACCGAATGCCGCA
GGTGTTGCAGCGTTTTCGGCAACGCGCTCCAAAAGTGCGTTTATCGTTGCAGTCGCTGAACTGTTACGTGATCCGTGATG
CGCTACTGAATGATGAGGCCGATGTCGGGGTTTTTTATCGCGTAGGGAATGATGATGTTCTGAACCGACGAGAGTTGGGT
GAACAACCGCTGGTGCTGGTGGCTTCACCGCAAATTGCAGATATCGATTTTACTGAGCCGGGAAGACATAACGCCTGTAG
CTTTATTATCAACGAACCACAATGTGTCTTCCGGCAGATATTTGAGAGCACGCTGCGCCAGCGGCGGATCACGGTGGAAA
ACACCATTGAGCTTATAAGTATCGAAAGCATCAAGCGTTGTGTCGCGGCAAATATCGGGGTCAGTTACCTGCCGCGCTTT
GCAGTGGGAAATGAACTTGCGAGTGGCGAATTAATCGAATTGCCTTTTGGCGAACAGACGCAGACCATTACGGCAATGTG
CGCTCACCATGCCGGAAAAGCGGTTAGTCCGGCGATGCACACTTTTATTCAGTGTGTTGAAGAGTGTTTTGTGGCTGGAT
AA

Upstream 100 bases:

>100_bases
GTTATCTCCTCTGTTTTGGTAGACAGTACCCGACACGGAACATTTAAAAAAATGAATGTTTTTACATTCTAAATTCGATA
TTTTCGATAGGTTTGGGGTT

Downstream 100 bases:

>100_bases
AAATAATGCCGGGCTTAAACCCGGCATTGAGATTACTCTTCTGTCGTTTGCGGTGGCACGCCCGTTCCGCTTGGTTTACG
GCGCTTACCAATATTTTTGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELG
EQPLVLVASPQIADIDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
AVGNELASGELIELPFGEQTQTITAMCAHHAGKAVSPAMHTFIQCVEECFVAG

Sequences:

>Translated_293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELG
EQPLVLVASPQIADIDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
AVGNELASGELIELPFGEQTQTITAMCAHHAGKAVSPAMHTFIQCVEECFVAG
>Mature_293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELG
EQPLVLVASPQIADIDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
AVGNELASGELIELPFGEQTQTITAMCAHHAGKAVSPAMHTFIQCVEECFVAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=293, Percent_Identity=96.9283276450512, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1788481, Length=296, Percent_Identity=26.0135135135135, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787806, Length=291, Percent_Identity=23.3676975945017, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=27.3026315789474, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=283, Percent_Identity=23.6749116607774, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI157672245, Length=137, Percent_Identity=31.3868613138686, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788706, Length=274, Percent_Identity=25.9124087591241, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788887, Length=300, Percent_Identity=24.3333333333333, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788296, Length=134, Percent_Identity=34.3283582089552, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33224; Mature: 33224

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEE
LSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELGEQPLVLVASPQIADIDFTEP
EEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCHHCHHHCCCCCEEEEECCCEEECCCCCC
GRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCHH
AVGNELASGELIELPFGEQTQTITAMCAHHAGKAVSPAMHTFIQCVEECFVAG
HCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEE
LSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELGEQPLVLVASPQIADIDFTEP
EEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCHHCHHHCCCCCEEEEECCCEEECCCCCC
GRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCHH
AVGNELASGELIELPFGEQTQTITAMCAHHAGKAVSPAMHTFIQCVEECFVAG
HCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837 [H]