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The map label for this gene is emrK [H]

Identifier: 157155084

GI number: 157155084

Start: 2645685

End: 2646848

Strand: Reverse

Name: emrK [H]

Synonym: EcE24377A_2654

Alternate gene names: 157155084

Gene position: 2646848-2645685 (Counterclockwise)

Preceding gene: 157156467

Following gene: 157157326

Centisome position: 53.15

GC content: 41.84

Gene sequence:

>1164_bases
GTGGAACAGATTAATTCAAATAAAAAACATTCTAACAGAAGAAAATACTTTTCTTTATTGGCGGTAGTTTTATTTATTGC
GTTTTCAGGTGCCTATGCCTATTGGTCAATGGAATTAGAAGACATGATTAGTACAGATGACGCCTATGTCACGGGGAATG
CAGATCCAATTTCTGCACAAGTCTCAGGTAGTGTCACTGTCGTTAATCATAAAGATACGAACTACGTTCGACAAGGTGAC
ATTTTAGTTTCACTGGATAAAACTGATGCCACTATCGCACTCAATAAAGCTAAAAATAATCTGGCAAATATTGTTCGGCA
AACGAATAAACTGTACTTACAGGATAAACAATACAGTGCCGAAGTCGCTTCAGCACGTATTCAGTATCAACAATCTTTAG
AAGATTATAACCGTCGAGTACCGTTAGCGAAGCAGGGGGTTATTTCAAAAGAAACGCTGGAGCATACCAAAGATACGTTA
ATAAGTAGCAAAGCGGCACTGAATGCCGCTATCCAGGCTTATAAAGCGAATAAAGCTTTAGTAATGAACACACCATTAAA
CCGTCAGCCACAAGTCGTGGAAGCGGCGGATGCAACTAAAGAAGCCTGGTTGGCGCTTAAACGTACGGATATAAGGAGTC
CGGTTACTGACTATATTGCCCAGCGGAGTGTTCAGGTCGGCGAAACAGTGAGTCCCGGACAATCGTTAATGGCTGTCGTA
CCGGCACGTCAAATGTGGGTTAATGCCAACTTTAAAGAAACACAACTCACGGATGTACGGATTGGTCAATCAGTCAATAT
TATCAGCGATCTTTATGGTGAAAATGTTGTGTTTCATGGTCGGGTGACAGGGATCAATATGGGAACCGGCAATGCGTTCT
CCTTATTACCTGCACAAAATGCGACAGGGAACTGGATCAAAATCGTTCAGCGTGTACCGGTTGAAGTTTCTCTTGATCCA
AAAGAACTCATGGAACACCCCTTACGCATTGGTTTATCGATGACTGCAACTATTGATACGAAGAACGAAGACATTGCTGA
GATGCCTGATCTGGCTTCAACCGTGACGTCCATGCCGGCTTATACCAGTAAGGCTTTAGTTATCGATACCAGTCCGATAG
AAAAAGAAATTAGCAACATTATTTCGCATAATGGACAACTTTAA

Upstream 100 bases:

>100_bases
CCTGGGATAATGTGCAACACATGCACTGTGTTTGATATGAAGAATGAATGCTCTTTTCATTCAATTCATAAATTTCATCT
ATGAGAAATGAGAGATAATA

Downstream 100 bases:

>100_bases
TGGCAATCACTAAATCGACTCCGGCACCATTAACCGGTGGGACGTTATGGTGCGTTACTATTGCATTGTCATTAGCGACG
TTTATGCAAATGTTGGATTC

Product: drug resistance MFS transporter, membrane fusion protein (MFP) subunit EmrK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTDYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL

Sequences:

>Translated_387_residues
MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTDYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL
>Mature_387_residues
MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTDYIAQRSVQVGETVSPGQSLMAVV
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDP
KELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL

Specific function: Unknown

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=387, Percent_Identity=99.2248062015504, Blast_Score=789, Evalue=0.0,
Organism=Escherichia coli, GI1789041, Length=378, Percent_Identity=49.2063492063492, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1790519, Length=324, Percent_Identity=27.7777777777778, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081951, Length=311, Percent_Identity=27.9742765273312, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789637, Length=310, Percent_Identity=26.1290322580645, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790024, Length=238, Percent_Identity=22.6890756302521, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42658; Mature: 42658

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCEEEECCCCCCEEE
VSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSA
ECCCEEEEECCCCCEEEECCEEEEECCCCCEEEEEHHHHHHHHHHHHHCCEEEECCHHHH
EVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKAL
HHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTDYIAQRSVQVGETVSPGQSLMAVV
EEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHEEEE
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQN
CCHHEEECCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCC
ATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSMPA
CCCHHHHHHHHCCEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHCCHHHHHHHHCCC
YTSKALVIDTSPIEKEISNIISHNGQL
CCCCEEEEECCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCEEEECCCCCCEEE
VSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSA
ECCCEEEEECCCCCEEEECCEEEEECCCCCEEEEEHHHHHHHHHHHHHCCEEEECCHHHH
EVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKAL
HHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTDYIAQRSVQVGETVSPGQSLMAVV
EEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHEEEE
PARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQN
CCHHEEECCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEEEEEECCCCCEEEEEECCC
ATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSMPA
CCCHHHHHHHHCCEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHCCHHHHHHHHCCC
YTSKALVIDTSPIEKEISNIISHNGQL
CCCCEEEEECCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]