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The map label for this gene is yafC

Identifier: 157155058

GI number: 157155058

Start: 231966

End: 232880

Strand: Reverse

Name: yafC

Synonym: EcE24377A_0212

Alternate gene names: 157155058

Gene position: 232880-231966 (Counterclockwise)

Preceding gene: 157157200

Following gene: 157158405

Centisome position: 4.68

GC content: 49.51

Gene sequence:

>915_bases
ATGAAAGCCACGTCGGAAGAACTCGCCATTTTTGTTTCGGTCGTCGAAAGCGGCAGCTTTAGCCGGGCAGCGGAACAATT
AGGGCAAGCAAACTCAGCGGTAAGCCGGGCAGTGAAAAAGCTGGAGATGAAACTTGGCGTTAGCCTGCTTAATCGGACCA
CGCGACAACTTAGCCTGACGGAAGAAGGCGAGCGTTATTTTCGTCGCGTACAGTCAATTTTGCAGGAGATGGCAGCGGCA
GAATCAGAAATTATGGAGACGCGTAATACACCGCGTGGACTGTTGCGCATCGATGCCGCAACTCCAGTGGTGCTGCACTT
TCTGATGCCGTTGATTAAGCCTTTCCGTGAACGCTATCCGGAAGTCACTTTGTCGCTAGTCTCCTCCGAAACGATTATTA
ATTTGATCGAAAGAAAAGTTGATGTCGCGATACGCGCCGGTACGTTAACGGATTCCAGCTTACGTGCCAGGCCGTTATTT
AATAGTTATCGAAAAATTATCGCCTCCCCCGATTATATTTCCCGCTACGGGAAGCCAGAAACGATCGACGATTTAAAGCA
ACATATTTGCCTGGGATTCACTGAACCCGCTTCCCTCAATACCTGGCCGATAGCCCGTAGCGATGGACAATTACATGAGG
TGAAGTACGGTTTGTCATCCAATAGTGGGGAAACACTGAAACAGCTTTGCCTGAGTGGCAACGGGATTGCGTGTTTGTCC
GACTACATGATCGACAAAGAAATCGCTCGCGGAGAATTGGTGGAGTTAATGGCAGATAAAGTGTTGCCAGTGGAAATGCC
ATTCAGTGCAGTCTATTACAGCGACCGTGCGGTAAGTACGCGCATCCGGGCTTTTATCGATTTCCTTAGCGAGCATGTAA
AAACAGCTCCCGGAGGAGCTGTCAGAGAGGCTTAA

Upstream 100 bases:

>100_bases
CATTGTTGAGAACATTAAATCGGATGACATATCACGCATCCTGATGGTTTAATGACAAATAAATTCAAAATATCTCTTCT
ATTTTGCAACAGGAGCAAAA

Downstream 100 bases:

>100_bases
TCCCATTCAGGAGCCAGACCTTCCGGGCTAACCAGGCGGTCGTTGCAATCCAGTGCGGCGATCGCTTTTTTATCTTCGGC
ATCAAGCTGTAAATTTTGTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA

Sequences:

>Translated_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA
>Mature_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=100, Blast_Score=621, Evalue=1e-179,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=33.5640138408304, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=28.7162162162162, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=29.3515358361775, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=31.5972222222222, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=28.6206896551724, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=28.8194444444444, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1789173, Length=284, Percent_Identity=28.8732394366197, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=26.1744966442953, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI157672245, Length=171, Percent_Identity=32.7485380116959, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788481, Length=303, Percent_Identity=25.7425742574257, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082132, Length=313, Percent_Identity=26.8370607028754, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786448, Length=254, Percent_Identity=26.7716535433071, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI145693105, Length=165, Percent_Identity=28.4848484848485, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788748, Length=126, Percent_Identity=34.1269841269841, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786508, Length=263, Percent_Identity=26.2357414448669, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790262, Length=245, Percent_Identity=24.4897959183673, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787806, Length=169, Percent_Identity=25.4437869822485, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789506, Length=281, Percent_Identity=25.9786476868327, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787530, Length=268, Percent_Identity=26.1194029850746, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YAFC_ECOLI (P30864)

Other databases:

- EMBL:   D12650
- EMBL:   U70214
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JS0716
- RefSeq:   AP_000863.1
- RefSeq:   NP_414744.1
- ProteinModelPortal:   P30864
- SMR:   P30864
- DIP:   DIP-11210N
- IntAct:   P30864
- MINT:   MINT-1323766
- STRING:   P30864
- EnsemblBacteria:   EBESCT00000000833
- EnsemblBacteria:   EBESCT00000016586
- GeneID:   947507
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0198
- KEGG:   eco:b0208
- EchoBASE:   EB1602
- EcoGene:   EG11649
- eggNOG:   COG0583
- GeneTree:   EBGT00050000008331
- HOGENOM:   HBG685425
- OMA:   RERYPEM
- ProtClustDB:   CLSK879575
- BioCyc:   EcoCyc:EG11649-MONOMER
- Genevestigator:   P30864
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33776; Mature: 33776

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYISRYGKPE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCC
TIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHHHCCCCHHHHH
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCC
VREA
CCCC
>Mature Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYISRYGKPE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCC
TIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHHHCCCCHHHHH
DYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCC
VREA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503