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The map label for this gene is menA

Identifier: 157154893

GI number: 157154893

Start: 4447097

End: 4448023

Strand: Reverse

Name: menA

Synonym: EcE24377A_4466

Alternate gene names: 157154893

Gene position: 4448023-4447097 (Counterclockwise)

Preceding gene: 157155037

Following gene: 157156032

Centisome position: 89.32

GC content: 53.72

Gene sequence:

>927_bases
ATGACTGAACAACAAATTAGCCGAACTCAGGCGTGGCTGGAAAGTTTACGACCTAAAACCCTCCCCCTCGCCTTTGCTGC
AATTATCGTCGGGACGGCGCTGGCATGGTGGCAAGGTCACTTCGATCCGCTGGTCGCCCTGCTGGCACTGATTACCGCCG
GGCTATTACAGATCCTTTCTAACCTCGCCAATGATTACGGCGATGCGGTAAAAGGCAGCGATAAACCTGACCGCATTGGG
CCGCTACGCGGCATGCAAAAAGGGGTCATTACCCAGCAAGAGATGAAACGGGCGCTCATTATTACCGTCGTGCTCATCTG
TCTCTCCGGGCTGGCACTGGTTGCAGTGGCATGCCACACGCTGGCCGATTTTGTCGGTTTCCTGATTCTTGGCGGGTTGT
CGATCATTGCCGCTATCACCTACACCGTGGGCAATCGTCCTTATGGTTATATCGGTCTGGGTGATATTTCCGTACTGGTT
TTCTTTGGCTGGTTGAGTGTCATGGGGAGCTGGTATTTACAGGCTCATACATTGATTCCGGCACTGATCCTTCCGGCGAC
CGCCTGTGGCCTGCTGGCAACGGCAGTACTGAATATTAATAACCTGCGTGATATCAATAGCGACCGCGAAAATGGCAAAA
ACACGCTGGTGGTGCGCTTAGGTGAAGTGAACGCGCGTCGTTATCATGCCTGCCTGCTGATGGGCTCGCTGGTGTGTCTG
GCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGC
CCGTTATGTGATGCGGGAAATGGACCCGGTGGCGATGCGACCAATGCTGGAACGTACTGTCAAGGGAGCGTTACTGACTA
ACCTGCTGTTTGTTTTAGGGATATTCCTAAGCCAGTGGGCAGCATAA

Upstream 100 bases:

>100_bases
GGCAGATGAAGATCTGAGCCGTTTTATCCTATAATCGCGTTCAATCATTTTCATCATTGTTTGATGGGGCTGAAAGGCCC
CATTTTTATTGGCGCGTATT

Downstream 100 bases:

>100_bases
CTGACAAATATCAATTAACAATTGATGATTTTGCCAACAGCCTACATAGCGCGATATACTGAAAATTCTCGCAGCAACTG
AATGTTAAGCCTATGAAATA

Product: 1,4-dihydroxy-2-naphthoate octaprenyltransferase

Products: NA

Alternate protein names: DHNA-octaprenyltransferase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPLVALLALITAGLLQILSNLANDYGDAVKGSDKPDRIG
PLRGMQKGVITQQEMKRALIITVVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVVRLGEVNARRYHACLLMGSLVCL
ALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDPVAMRPMLERTVKGALLTNLLFVLGIFLSQWAA

Sequences:

>Translated_308_residues
MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPLVALLALITAGLLQILSNLANDYGDAVKGSDKPDRIG
PLRGMQKGVITQQEMKRALIITVVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVVRLGEVNARRYHACLLMGSLVCL
ALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDPVAMRPMLERTVKGALLTNLLFVLGIFLSQWAA
>Mature_307_residues
TEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPLVALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGP
LRGMQKGVITQQEMKRALIITVVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLVF
FGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVVRLGEVNARRYHACLLMGSLVCLA
LFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDPVAMRPMLERTVKGALLTNLLFVLGIFLSQWAA

Specific function: Conversion of 1,4-dihydroxy-2-naphthoate (DHNA) to dimethylmenaquinone (DMK). Attaches octaprenylpyrophosphate, a membrane-bound 40-carbon side chain to DHNA. The conversion of DHNA to DMK proceeds in three stages:the removal of the carboxyl group of DHNA

COG id: COG1575

COG function: function code H; 1,4-dihydroxy-2-naphthoate octaprenyltransferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the menA family

Homologues:

Organism=Homo sapiens, GI7019551, Length=308, Percent_Identity=29.8701298701299, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1790365, Length=308, Percent_Identity=100, Blast_Score=618, Evalue=1e-178,
Organism=Drosophila melanogaster, GI17737429, Length=302, Percent_Identity=27.8145695364238, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENA_ECOLI (P32166)

Other databases:

- EMBL:   U56082
- EMBL:   L19201
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S40873
- RefSeq:   AP_003879.1
- RefSeq:   NP_418365.1
- ProteinModelPortal:   P32166
- STRING:   P32166
- EnsemblBacteria:   EBESCT00000002335
- EnsemblBacteria:   EBESCT00000017721
- GeneID:   948418
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3901
- KEGG:   eco:b3930
- EchoBASE:   EB1826
- EcoGene:   EG11880
- eggNOG:   COG1575
- GeneTree:   EBGT00050000010583
- HOGENOM:   HBG313998
- OMA:   RVTQSGM
- ProtClustDB:   PRK06080
- BioCyc:   EcoCyc:DMK-MONOMER
- BioCyc:   MetaCyc:DMK-MONOMER
- Genevestigator:   P32166
- InterPro:   IPR004657
- InterPro:   IPR000537
- TIGRFAMs:   TIGR00751

Pfam domain/function: PF01040 UbiA

EC number: =2.5.1.74

Molecular weight: Translated: 33595; Mature: 33463

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x22657598)-; HASH(0x21bf6168)-; HASH(0x225f1d58)-; HASH(0x2262e204)-; HASH(0x22675c68)-; HASH(0x21d656c4)-; HASH(0x21b24984)-; HASH(0x21f5961c)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPLVALLALITAGLLQILS
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
NLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIITVVLICLSGLALVAVACHT
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLVFFGWLSVMGSWYLQAHTLIP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALILPATACGLLATAVLNINNLRDINSDRENGKNTLVVRLGEVNARRYHACLLMGSLVCL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
ALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDPVAMRPMLERTVKGALLTNLLFVLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
IFLSQWAA
HHHHHHCH
>Mature Secondary Structure 
TEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPLVALLALITAGLLQILS
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
NLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIITVVLICLSGLALVAVACHT
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLVFFGWLSVMGSWYLQAHTLIP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALILPATACGLLATAVLNINNLRDINSDRENGKNTLVVRLGEVNARRYHACLLMGSLVCL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
ALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDPVAMRPMLERTVKGALLTNLLFVLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
IFLSQWAA
HHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9573170; 8346018; 9278503