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The map label for this gene is rutD [H]

Identifier: 157154886

GI number: 157154886

Start: 1148615

End: 1149415

Strand: Reverse

Name: rutD [H]

Synonym: EcE24377A_1127

Alternate gene names: 157154886

Gene position: 1149415-1148615 (Counterclockwise)

Preceding gene: 157155569

Following gene: 157156722

Centisome position: 23.08

GC content: 58.05

Gene sequence:

>801_bases
ATGAAACTATCACTCTCACCTCCCCCTTATGCTGATGCACCCGTAGTGGTGTTGATTTCGGGTCTTGGCGGTAGCGGCAG
TTACTGGTTACCGCAACTGGCGGTGCTGGAGCAGGAGTATCAGGTAGTCTGTTACGACCAGCGCGGCACCGGCAATAATC
CCGACACGCTGGCAGAAGATTACAGTATCACCCAGATGGCAGCGGAACTGCATCAGGCGCTGGTAGCCGCAGGGATTGAG
CGTTACGCGGTGGTCGGCCATGCGCTCGGTGCGCTGGTGGGAATGCAGCTGGCGCTGGATTATCCCGCGTCGGTAACTGT
GCTGATCAGCGTTAACGGCTGGCTACGAATAAACGCCCATACGCGCCGCTGTTTTCAGGTTCGCGAACGATTACTGTATA
GCGGCGGCGCGCAGGCATGGGTGGAAGCGCAGCCGTTGTTCCTCTATCCCGCCGACTGGATGGCGGCCCGCGCACCTCGC
CTTGAGGCAGAAGACGCGCTGGCACTGGCGCATTTTCAGGGCAAAAATAATTTACTGCGTCGACTTAACGCCCTCAAACG
CGCTGACTTTAGTCACCATGCGGATCGCATCCGCTGCCCGGTGCAAATCATCTGCGCCAGTGATGATCTGCTGGTGCCAT
CAGCATGTTCCAGTGAGCTTCATGCCGCCCTGCCCGATAGCCAGAAAATGGTGATGCGCTATGGCGGACACGCCTGCAAC
GTGACCGACCCCGAAACGTTTAATGCTCTTTTACTCAACGGGCTTGCCAGCCTGTTACATCACCGCGAAGCCGCCCTGTA
A

Upstream 100 bases:

>100_bases
GGTGATAAACCGGCGCGATTCTGCATTCAGTGCGGACTGGTAAAACCTGACGCGCTGGTGGAAATCGCCACAATTGCGCA
TATCGCCAAGTGAGGCCGCG

Downstream 100 bases:

>100_bases
GGAATTGCTATGAACGAAGCCGTTAGCCCAGGTGCGCTTAGCACCCTGTTCACCGATGCCCGCACTCACAACGGCTGGCG
GGAGACACCCGTCAGCGATG

Product: putative rutD protein

Products: NA

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIE
RYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMVMRYGGHACN
VTDPETFNALLLNGLASLLHHREAAL

Sequences:

>Translated_266_residues
MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIE
RYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMVMRYGGHACN
VTDPETFNALLLNGLASLLHHREAAL
>Mature_266_residues
MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIE
RYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMVMRYGGHACN
VTDPETFNALLLNGLASLLHHREAAL

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

Organism=Escherichia coli, GI1787244, Length=266, Percent_Identity=98.4962406015038, Blast_Score=535, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 28993; Mature: 28993

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAED
CCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHC
YSITQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEEECCEEEECHH
TRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPRLEAEDALALAHFQGKNNLLR
HHHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHCCCCCCCHHHEEEEHCCCHHHHHH
RLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMVMRYGGHACN
HHHHHHHCCCHHCHHHCCCCEEEEECCCCEECCCCCCCHHHHCCCCHHHHHHHHCCCCCC
VTDPETFNALLLNGLASLLHHREAAL
CCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVCYDQRGTGNNPDTLAED
CCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHC
YSITQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYPASVTVLISVNGWLRINAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEEECCEEEECHH
TRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPRLEAEDALALAHFQGKNNLLR
HHHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHCCCCCCCHHHEEEEHCCCHHHHHH
RLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSSELHAALPDSQKMVMRYGGHACN
HHHHHHHCCCHHCHHHCCCCEEEEECCCCEECCCCCCCHHHHCCCCHHHHHHHHCCCCCC
VTDPETFNALLLNGLASLLHHREAAL
CCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA