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The map label for this gene is ttg2D

Identifier: 157154788

GI number: 157154788

Start: 3674413

End: 3675048

Strand: Reverse

Name: ttg2D

Synonym: EcE24377A_3679

Alternate gene names: 157154788

Gene position: 3675048-3674413 (Counterclockwise)

Preceding gene: 157156204

Following gene: 157157685

Centisome position: 73.8

GC content: 52.52

Gene sequence:

>636_bases
ATGTTTAAACGTTTAATGATGGTCGCTTTGCTGGTGATTGCACCTCTGAGTGCGGCAACCGCGGCAGACCAGACCAATCC
GTATAAGCTGATGGACGAGGCGGCGCAGAAAACGTTCGATCGCCTGAAGAATGAGCAACCGCAAATTCGGGCCAACCCGG
ATTATCTGCGTACCATTGTTGATCAGGAACTGCTGCCATACGTACAGGTGAAATACGCCGGTGCGCTGGTGCTGGGCCAG
TATTACAAGAGTGCGACCCCTGCTCAACGTGAAGCCTACTTTGCCGCTTTCCGTGAGTACCTGAAGCAGGCTTACGGTCA
GGCGCTGGCGATGTATCACGGTCAAACCTATCAGATTGCGCCAGAACAGCCGCTGGGCGATAAAACCATTGTGCCTATTC
GCGTTACCATTATTGATCCGAATGGCCGCCCGCCGGTGCGTCTGGACTTCCAGTGGCGTAAAAACTCCCAGACGGGCAAC
TGGCAGGCTTACGACATGATTGCTGAAGGCGTCAGTATGATCACCACCAAACAAAACGAGTGGGGAACGCTGCTGCGTAC
CAAAGGTATCGACGGCCTGACTGCGCAACTGAAATCGATTTCTCAACAGAAAATCACTCTGGAAGAGAAAAAATAA

Upstream 100 bases:

>100_bases
TGACAATAAGAATAGTGGCGATGCGCCAGCTGCTGCGCCAGGTAATAATGAAACCACTGAACCTGTGGGTACAACGAAAT
AATTTCAGGAGAACCGACGC

Downstream 100 bases:

>100_bases
TGAGCGAGTCACTGAGCTGGATGCAGACGGGTGACACGCTGGCGTTATCCGGAGAGCTGGATCAGGACGTTTTGCTACCG
CTTTGGGAAATGCGTGAGGA

Product: toluene tolerance protein Ttg2D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKNEQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQ
YYKSATPAQREAYFAAFREYLKQAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK

Sequences:

>Translated_211_residues
MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKNEQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQ
YYKSATPAQREAYFAAFREYLKQAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK
>Mature_211_residues
MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKNEQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQ
YYKSATPAQREAYFAAFREYLKQAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane. May transfer phospholipid across the peripl

COG id: COG2854

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, auxiliary component

Gene ontology:

Cell location: Periplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaC/ttg2D family

Homologues:

Organism=Escherichia coli, GI1789583, Length=211, Percent_Identity=100, Blast_Score=433, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLAC_ECOLI (P0ADV7)

Other databases:

- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B65110
- RefSeq:   AP_003735.1
- RefSeq:   NP_417659.1
- ProteinModelPortal:   P0ADV7
- SMR:   P0ADV7
- STRING:   P0ADV7
- SWISS-2DPAGE:   P0ADV7
- EnsemblBacteria:   EBESCT00000002040
- EnsemblBacteria:   EBESCT00000016035
- GeneID:   947710
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3159
- KEGG:   eco:b3192
- EchoBASE:   EB2650
- EcoGene:   EG12798
- eggNOG:   COG2854
- GeneTree:   EBGT00050000009442
- HOGENOM:   HBG430191
- OMA:   VHIKYAG
- ProtClustDB:   PRK15117
- BioCyc:   EcoCyc:G7659-MONOMER
- Genevestigator:   P0ADV7
- InterPro:   IPR023094
- InterPro:   IPR008869

Pfam domain/function: PF05494 Tol_Tol_Ttg2

EC number: NA

Molecular weight: Translated: 23963; Mature: 23963

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKNEQPQIRANPDYLRTIV
CHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
DQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLKQAYGQALAMYHGQTYQIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGNWQAYDMIAEGVSMITTKQNE
CCCCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHCCHHHEEECCCC
WGTLLRTKGIDGLTAQLKSISQQKITLEEKK
CCHHHEECCCCHHHHHHHHHHHHHCEECCCC
>Mature Secondary Structure
MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKNEQPQIRANPDYLRTIV
CHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
DQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLKQAYGQALAMYHGQTYQIA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGNWQAYDMIAEGVSMITTKQNE
CCCCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHCCHHHEEECCCC
WGTLLRTKGIDGLTAQLKSISQQKITLEEKK
CCHHHEECCCCHHHHHHHHHHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503