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The map label for this gene is ybeF [H]

Identifier: 157154784

GI number: 157154784

Start: 683099

End: 684052

Strand: Reverse

Name: ybeF [H]

Synonym: EcE24377A_0654

Alternate gene names: 157154784

Gene position: 684052-683099 (Counterclockwise)

Preceding gene: 157155500

Following gene: 157156510

Centisome position: 13.74

GC content: 44.97

Gene sequence:

>954_bases
GTGGATAGTAATAATCAAATTGAACCTTGTTTAAGCAGAAAGTCGTCAGAAGGTAAACCACAAATATTTACAACCTTACG
AAATATTGATCTTAACCTTCTGACTATTTTTGAAGCTGTATATGTACATAAAGGGATCGTTAATGCAGCGAAAGTGCTTA
ATCTGACCCCCTCGGCAATCAGTCAGTCTATTCAGAAACTGCGCGTTATATTCCCTGACCCATTGTTTATTCGCAAAGGC
CAGGGTGTCACTCCTACCGCATTTGCGATGCATCTACATGAGTATATCAGTCAGGGCCTTGAGTCCATTCTTGGCGCGCT
GGATATCGAAGGAAGCTATGATAAGCAACGAACGATAACTATTGCGACCACTCCCTCGGTCGGAGCCCTGGTCCTTCCTG
TCATCTATCGGGCGATTAAAACTCACTATCCGCAGCTTTTACTGCGCAACCCGCCCATCAGCGACGCGGAAAACCAACTC
AGTCAGTTTCAAACCGATCTCATCATCGATAACATGTTTTGCACCAATCGTACGGTGCAACATCATGTTCTGTTCACCGA
CGATATGGTGTTAATTTGCCGCGAGGGAAATCCACTACTCTCTTTAGAAGATGACAGAGAGACTATCGACAACGCTGCGC
ATGTACTTCTGTTACCGGAAGAGCAAAATTTCAGCGGTCTGCGGCAGAGAGTTCAAGAAATGTTTCCGGACCGGCAAATC
AATTTCACCAGCTACAACATTTTGACAATCGCTGCACTGGTTGCCAACAGTGACATGTTAGCGATTATTCCCAGCCGTTT
TTATAACCTGTTTAGCCGCTGCTGGCCGCTGGAAAAACTGCCTTTTCCGTCCTTAAATGAGGAGCAAATAGATTTCTCTA
TCCACTACAACAAATTCAGCCTGCGTGATCCCATCCTGCACGGAGTAATAGATGTTATCCGTAATGCATTTTGA

Upstream 100 bases:

>100_bases
GTTGATTATGGTGAGCAAAAGCGTTCCGTTCCAATTAGACATAAGATTATTGTAATTTTTTTAAATATTATTTTTCCATG
AATAAAATTGGAGTAAGTGC

Downstream 100 bases:

>100_bases
AAAAGTATTGTTACAGCCAGATCACAGCGCAGAACGATAAAATGGCACAATCCAACAACAATTTTACATTTTCGCGACCG
CTTTGGCTGCTTTCAGGTCC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKG
QGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
SQFQTDLIIDNMFCTNRTVQHHVLFTDDMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQI
NFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF

Sequences:

>Translated_317_residues
MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKG
QGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
SQFQTDLIIDNMFCTNRTVQHHVLFTDDMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQI
NFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF
>Mature_317_residues
MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKG
QGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
SQFQTDLIIDNMFCTNRTVQHHVLFTDDMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQI
NFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081766, Length=317, Percent_Identity=99.6845425867508, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI1786820, Length=299, Percent_Identity=30.1003344481605, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI87081684, Length=272, Percent_Identity=26.4705882352941, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790147, Length=296, Percent_Identity=23.3108108108108, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35962; Mature: 35962

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAI
CCCCCCCCHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTIT
HHHHHHHHEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
IATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQ
EEECCCCHHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHCHHHCCCCEE
HHVLFTDDMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQI
EEEEEECCEEEEEECCCCEEEECCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEE
NFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFS
CEEEHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECEEC
LRDPILHGVIDVIRNAF
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAI
CCCCCCCCHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTIT
HHHHHHHHEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
IATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQ
EEECCCCHHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHCHHHCCCCEE
HHVLFTDDMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDRQI
EEEEEECCEEEEEECCCCEEEECCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEE
NFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFS
CEEEHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECEEC
LRDPILHGVIDVIRNAF
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8444795; 8905232; 9278503 [H]