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The map label for this gene is paaF [C]

Identifier: 157163673

GI number: 157163673

Start: 4457898

End: 4458674

Strand: Reverse

Name: paaF [C]

Synonym: EcHS_A4452

Alternate gene names: 157163673

Gene position: 4458674-4457898 (Counterclockwise)

Preceding gene: 157163674

Following gene: 157163672

Centisome position: 96.02

GC content: 49.94

Gene sequence:

>777_bases
ATGAGTGACCAACCCGTTCTGTTCAGCAGAGTGGCGGCTTCCTGCCGCCTGACATTGAACCGTGAAGATAAATGTCATGC
TATTAACGAAGAGATGATCGAATCTCTCGACCATTATTTAAATGAAATTGAAAACGACACCACATTACGATTAGTCGAAT
TAACGGCAACCGGCGATAAATTCTTTTGTGCGGGCGGCGATATTAAATCGTGGTCGGCCTATTCGCCATTAGATATGGGC
AGAAAATGGATTAAACGCGGTAATGACGTTTTTAATCGCCTGCGAAACATACCCCAATTAACCGTTGCGAATCTCAACGG
TCATACCATCGGCGGTGGTATTGAACTGGCCTTGTGCTGCGATATTCGTATTGCCCGCCCCGGCGCGAAATTCTCTAACC
CGGAAGTGATGCTCGGCATGGTGCCTGGCTGGATGGGCATTGAACGTGTGCTTAATCAGGTTGGTCCGGTGGTCGGTCGC
CAGATGCTGATGCTGGGTAAACGCCTGACGGCGCAGGAAGCCCAGGCGGCGAATTTAATTGATGAAGTGGTAGAAAAAGA
ACAGGTGGAAAGCTGGATGGCGAACCAGTTAGCGCAGCTGGAAAAATGCGGTCCGGTGGCACTGGCGCATATTAAGCAAC
TGATTCTGGCACTGGAAAATAAACATGCCGATTACCCGCACCAGCTACTGGCCGGATTAATGTCTGCCACTCAGGATTGC
CAGCAGGCGACGCGCGCCTTTGCCGAAAAAAGTAGCGTGAGCTTCCATAATCAATAA

Upstream 100 bases:

>100_bases
TGAACTGGAAATATTCTCGCCTTACTGGTGGCAGAAAGATATTAACAGCACGCTGGATATTAGCGTCGATCGCATCGCGC
ATTATTGTTAAGGAATGATC

Downstream 100 bases:

>100_bases
GGAAATTGTATGCGTAAGATAACAACCGCCGAGGCACTGGCGGCGCAAATTCAGGACGGTGCGACTATTGCTATTAGCGG
TAACGGCGGCGGTATGGTGG

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMG
RKWIKRGNDVFNRLRNIPQLTVANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQLLAGLMSATQDC
QQATRAFAEKSSVSFHNQ

Sequences:

>Translated_258_residues
MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMG
RKWIKRGNDVFNRLRNIPQLTVANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQLLAGLMSATQDC
QQATRAFAEKSSVSFHNQ
>Mature_257_residues
SDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGR
KWIKRGNDVFNRLRNIPQLTVANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGRQ
MLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQLLAGLMSATQDCQ
QATRAFAEKSSVSFHNQ

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=29.9212598425197, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI20127408, Length=164, Percent_Identity=29.2682926829268, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI68989263, Length=183, Percent_Identity=28.9617486338798, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI70995211, Length=246, Percent_Identity=26.4227642276423, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI62530384, Length=177, Percent_Identity=29.3785310734463, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI37594469, Length=188, Percent_Identity=25.531914893617, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1787659, Length=247, Percent_Identity=28.7449392712551, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI221142681, Length=252, Percent_Identity=26.984126984127, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1790281, Length=177, Percent_Identity=28.2485875706215, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=27.2357723577236, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=25.6603773584906, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788682, Length=164, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787660, Length=249, Percent_Identity=31.7269076305221, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=30.1960784313725, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=192, Percent_Identity=28.125, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=237, Percent_Identity=24.0506329113924, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=184, Percent_Identity=26.6304347826087, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=24.1106719367589, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=199, Percent_Identity=24.1206030150754, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=220, Percent_Identity=26.3636363636364, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=129, Percent_Identity=30.2325581395349, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=129, Percent_Identity=30.2325581395349, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=129, Percent_Identity=30.2325581395349, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=255, Percent_Identity=25.8823529411765, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=193, Percent_Identity=26.9430051813472, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=193, Percent_Identity=26.9430051813472, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17558304, Length=164, Percent_Identity=26.219512195122, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=196, Percent_Identity=28.0612244897959, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=30.7086614173228, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=30.7086614173228, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24653139, Length=249, Percent_Identity=24.0963855421687, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28571730, Length=191, Percent_Identity=25.6544502617801, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571729, Length=191, Percent_Identity=25.6544502617801, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583165, Length=201, Percent_Identity=29.8507462686567, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921018, Length=229, Percent_Identity=28.3842794759825, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24654903, Length=201, Percent_Identity=24.8756218905473, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28613; Mature: 28482

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTTLRLVELTATGDK
CCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCE
FFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTVANLNGHTIGGGIELALCC
EEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCEEECCCEEEEEEE
DIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGRQMLMLGKRLTAQEAQAANLI
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQLLAGLMSATQDC
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QQATRAFAEKSSVSFHNQ
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTTLRLVELTATGDK
CCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCE
FFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTVANLNGHTIGGGIELALCC
EEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCEEECCCEEEEEEE
DIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGRQMLMLGKRLTAQEAQAANLI
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQLLAGLMSATQDC
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QQATRAFAEKSSVSFHNQ
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA