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The map label for this gene is dcuA

Identifier: 157163603

GI number: 157163603

Start: 4389495

End: 4390796

Strand: Reverse

Name: dcuA

Synonym: EcHS_A4379

Alternate gene names: 157163603

Gene position: 4390796-4389495 (Counterclockwise)

Preceding gene: 157163604

Following gene: 157163602

Centisome position: 94.56

GC content: 52.53

Gene sequence:

>1302_bases
ATGCTAGTTGTAGAACTCATCATAGTTTTGCTGGCGATCTTCTTGGGCGCCAGATTGGGGGGAATAGGTATTGGTTTTGC
AGGCGGATTGGGGGTGCTGGTTCTTGCCGCTATTGGCGTTAAACCCGGTAACATCCCGTTCGATGTCATTTCCATTATCA
TGGCGGTTATCGCCGCTATTTCTGCCATGCAGGTTGCTGGCGGTCTGGACTATCTGGTTCATCAGACAGAAAAGCTGCTG
CGCCGTAACCCGAAATACATCACGATCCTCGCACCGATCGTGACCTATTTCCTGACTATCTTTGCTGGTACTGGCAACAT
ATCTCTGGCGACACTGCCAGTTATCGCTGAAGTTGCGAAGGAACAAGGCGTCAAACCTTGCCGTCCGCTGTCTACTGCAG
TGGTATCCGCGCAGATTGCGATCACCGCATCGCCAATCTCAGCGGCAGTGGTTTACATGTCTTCCGTGATGGAAGGTCAT
GGCATCAGCTACCTCCATCTGCTCTCCGTGGTCATCCCGTCCACCCTGCTGGCGGTTCTGGTGATGTCCTTTCTGGTCAC
TATGCTGTTCAACTCCAAACTCTCTGACGATCCGATTTATCGCAAGCGTCTGGAAGAGGGCCTGGTTGAACTGCGCGGTG
AAAAGCAGATTGAAATCAAATCCGGTGCAAAAACGTCCGTCTGGCTGTTCCTGCTGGGCGTAGTTGGCGTGGTTATCTAT
GCAATCATCAACAGCCCAAGCATGGGTCTGGTTGAAAAACCGCTGATGAACACCACCAACGCAATCCTGATCATCATGCT
CAGCGTTGCAACTCTGACCACCGTTATCTGTAAAGTCGATACCGACAACATCCTCAACTCCAGCACCTTCAAAGCAGGTA
TGAGCGCCTGTATTTGTATCCTGGGTGTTGCGTGGCTGGGCGATACTTTCGTTTCCAACAACATCGACTGGATCAAAGAT
ACCGCTGGTGAAGTGATTCAGGGTCATCCGTGGCTGCTGGCCGTCATCTTCTTCTTTGCTTCTGCTCTGCTGTACTCTCA
GGCTGCAACCGCAAAAGCACTGATGCCGATGGCCCTGGCACTGAACGTTTCACCGCTGACCGCTGTTGCTTCTTTCGCTG
CGGTGTCTGGTCTGTTCATTCTGCCGACCTACCCGACGCTGGTTGCTGCGGTACAGATGGATGACACGGGTACAACCCGT
ATCGGTAAATTCGTCTTCAACCATCCGTTCTTCATCCCGGGTACTCTGGGTGTTGCCCTGGCCGTTTGCTTCGGCTTCGT
GCTGGGTAGCTTCATGCTGTAA

Upstream 100 bases:

>100_bases
AATAAAGAAGGCACGTCAGATGACGTGCCTTTTTTCTTGTGAGCAGTAACTTAAAAATAACAACCTAATATCAACTTGTT
AAAAAACAAGGAAGGCTAAT

Downstream 100 bases:

>100_bases
TGACCAATCGCGGGGCGTTCACGCCCCGCTTTCTTTCCCGCCGACTAACATCCTTCCCCCGTCCGTTGTATAGTGACCTC
TCTCTTGCGGTTCCATCTGT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGNIPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLL
RRNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIEIKSGAKTSVWLFLLGVVGVVIY
AIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVICKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKD
TAGEVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML

Sequences:

>Translated_433_residues
MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGNIPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLL
RRNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIEIKSGAKTSVWLFLLGVVGVVIY
AIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVICKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKD
TAGEVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML
>Mature_433_residues
MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGNIPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLL
RRNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIEIKSGAKTSVWLFLLGVVGVVIY
AIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVICKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKD
TAGEVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family

Homologues:

Organism=Escherichia coli, GI1790580, Length=433, Percent_Identity=100, Blast_Score=859, Evalue=0.0,
Organism=Escherichia coli, GI1790565, Length=404, Percent_Identity=36.1386138613861, Blast_Score=242, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCUA_ECO57 (P0ABN7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E86109
- PIR:   G91268
- RefSeq:   NP_290771.1
- RefSeq:   NP_313146.1
- ProteinModelPortal:   P0ABN7
- EnsemblBacteria:   EBESCT00000025030
- EnsemblBacteria:   EBESCT00000055269
- GeneID:   914130
- GeneID:   959985
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5743
- KEGG:   ecs:ECs5119
- GeneTree:   EBGT00050000010823
- HOGENOM:   HBG297083
- OMA:   FYAPIVT
- ProtClustDB:   PRK09412
- BioCyc:   ECOL83334:ECS5119-MONOMER
- InterPro:   IPR004668
- PIRSF:   PIRSF004539
- TIGRFAMs:   TIGR00770

Pfam domain/function: PF03605 DcuA_DcuB

EC number: NA

Molecular weight: Translated: 45751; Mature: 45751

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x195b9028)-; HASH(0x19215654)-; HASH(0x1917ec08)-; HASH(0x18da1ff8)-; HASH(0x19523e24)-; HASH(0x191497c0)-; HASH(0x19021fd8)-; HASH(0x1953f054)-; HASH(0x193f7dd4)-; HASH(0x19331a80)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGNIPFDVISIIMAVIAAI
CHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAK
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGHGISYLHLLSVVIPSTLLAVL
HCCCCCCCHHHHHHHHEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIEIKSGAKTSVWLFLLGVVGVVIY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
AIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVICKVDTDNILNSSTFKAGMSACICI
HHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHH
LGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALA
HHHHHHHHHHHCCCCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTRIGKFVFNHPFFIPGTLGVAL
CCCCHHHHHHHHHHHCCEEEECCCHHHHEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHH
AVCFGFVLGSFML
HHHHHHHHHHHCH
>Mature Secondary Structure
MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGNIPFDVISIIMAVIAAI
CHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAK
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGHGISYLHLLSVVIPSTLLAVL
HCCCCCCCHHHHHHHHEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIEIKSGAKTSVWLFLLGVVGVVIY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
AIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVICKVDTDNILNSSTFKAGMSACICI
HHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHH
LGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALA
HHHHHHHHHHHCCCCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTRIGKFVFNHPFFIPGTLGVAL
CCCCHHHHHHHHHHHCCEEEECCCHHHHEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHH
AVCFGFVLGSFML
HHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796