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The map label for this gene is dcuS [H]

Identifier: 157163591

GI number: 157163591

Start: 4374053

End: 4375684

Strand: Reverse

Name: dcuS [H]

Synonym: EcHS_A4366

Alternate gene names: 157163591

Gene position: 4375684-4374053 (Counterclockwise)

Preceding gene: 157163595

Following gene: 157163590

Centisome position: 94.23

GC content: 50.18

Gene sequence:

>1632_bases
ATGAGACATTCATTGCCCTACCGCATGTTACGCAAACGTCCGATGAAATTGAGTACCACAGTGATCTTAATGGTCAGCGC
GGTACTGTTCTCGGTGCTATTGGTGGTGCATCTGATTTACTTCTCGCAAATCAGTGATATGACGCGAGACGGGCTGGCCA
ACAAGGCACTGGCAGTGGCGCGTACCCTCGCCGACTCGCCGGAAATCCGTCAGGGCTTGCAGAAAAAACCGCAGGAGAGC
GGAATCCAGGCCATCGCGGAAGCCGTGCGCAAACGCAACGATCTGCTGTTTATTGTCGTTACCGATATGCAAAGTCTTCG
CTACTCGCATCCTGAAGCCCAGCGTATTGGTCAGCCATTTAAAGGTGATGACATCCTTAAAGCGCTGAATGGCAAAGAAA
ATGTCGCTATCAATCGCGGTTTTCTGGCGCAGGCTTTACGCGTATTTACCCCCATCTACGATGAAAATCATAAACAAATT
GGCGTGGTGGCGATCGGCCTTGAGTTAAGCCGCGTGACCCAACAAATCAATGACAGTCGCTGGAGCATTATCTGGTCGGT
ATTATTTGGCATGCTGGTCGGACTGATTGGCACCTGCATTCTGGTTAAGGTACTGAAAAAAATCCTTTTCGGCCTGGAAC
CCTACGAAATCTCCACGCTGTTTGAGCAGCGCCAGGCCATGTTGCAGTCTATCAAAGAAGGCGTCGTTGCCGTGGACGAT
CGCGGCGAGGTCACGCTGATCAACGATGCCGCACAAGAATTGCTGAATTACCGTAAGTCGCAGGACGATGAGAAACTGTC
GACGCTAAGCCACTCATGGTCACAGGTGGTAGATGTCTCGGAAGTGTTACGCGACGGTACGCCGCGCCGCGACGAAGAGA
TTACGATTAAAGACCGGCTATTACTGATCAACACCGTTCCGGTGCGCAGTAATGGCGTTATCATCGGTGCCATTTCAACC
TTCAGGGACAAAACTGAAGTTCGTAAACTGATGCAGCGACTCGACGGTCTGGTCAACTATGCTGATGCACTTCGTGAACG
ATCCCACGAATTTATGAATAAATTGCATGTGATTCTCGGATTATTGCATCTGAAGAGTTATAAGCAGTTGGAAGATTACA
TTCTCAAAACAGCCAATAACTATCAGGAAGAGATTGGCTCTCTGCTGGGTAAGATCAAATCTCCGGTTATCGCTGGTTTT
TTAATCAGCAAGATTAACCGCGCGACCGATTTAGGCCATACGCTGATTTTAAACAGTGAAAGCCTGCTGCCAGACAGCGG
CAGTGAGGACCAGGTCGCGACGCTGATTACCACATTGGGAAATCTGATAGAAAACGCGCTGGAAGCATTAGGGCCGGAAC
CCGGTGGCGAAATTAGCGTAACATTGCACTACCGTCACGGCTGGCTGCACTGCGAAGTTAACGATGATGGACCGGGGATC
GCACCCGACAAAATCGATCACATTTTTGACAAAGGTGTCTCGACAAAAGGAAGCGAGCGAGGCGTCGGTTTAGCACTTGT
CAAACAACAGGTAGAAAATCTCGGCGGCAGCATCGCCGTGGAATCGGAACCCGGGATTTTCACACAATTTTTTGTCCAGA
TACCCTGGGACGGGGAGAGGTCGAACAGATGA

Upstream 100 bases:

>100_bases
CCAGAGTCTCTTCCCCTATATAGCCAATCATTCTTACTCCCTTTGAATTACCCGCCTCATCAGAGATAATGCTTAAGAAA
TTTGTCACACAAGGAAGCTG

Downstream 100 bases:

>100_bases
TCAATGTATTAATTATCGATGACGACGCAATGGTCGCGGAGCTGAATCGCCGATACGTAGCACAAATCCCAGGCTTTCAA
TGCTGTGGAACAGCCTCGAC

Product: sensory histidine kinase DcuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQISDMTRDGLANKALAVARTLADSPEIRQGLQKKPQES
GIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEISTLFEQRQAMLQSIKEGVVAVDD
RGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAIST
FRDKTEVRKLMQRLDGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKIKSPVIAGF
LISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEALGPEPGGEISVTLHYRHGWLHCEVNDDGPGI
APDKIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR

Sequences:

>Translated_543_residues
MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQISDMTRDGLANKALAVARTLADSPEIRQGLQKKPQES
GIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEISTLFEQRQAMLQSIKEGVVAVDD
RGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAIST
FRDKTEVRKLMQRLDGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKIKSPVIAGF
LISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEALGPEPGGEISVTLHYRHGWLHCEVNDDGPGI
APDKIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR
>Mature_543_residues
MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQISDMTRDGLANKALAVARTLADSPEIRQGLQKKPQES
GIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEISTLFEQRQAMLQSIKEGVVAVDD
RGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAIST
FRDKTEVRKLMQRLDGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKIKSPVIAGF
LISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEALGPEPGGEISVTLHYRHGWLHCEVNDDGPGI
APDKIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR

Specific function: Member of the two-component regulatory system dcuR/dcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-di

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=543, Percent_Identity=99.6316758747698, Blast_Score=1100, Evalue=0.0,
Organism=Escherichia coli, GI1786837, Length=542, Percent_Identity=27.1217712177122, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1790300, Length=346, Percent_Identity=26.878612716763, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=28.2700421940928, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786600, Length=352, Percent_Identity=24.4318181818182, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60536; Mature: 60536

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQISDMTRDGLANKALAVA
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQRIGQPF
HHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCCHHHCCCCC
KGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQIGVVAIGLELSRVTQQINDSR
CCCHHHHHHCCCCCEEECHHHHHHHHHHHCCHHCCCCCEEEEEEECHHHHHHHHHHCCHH
WSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEISTLFEQRQAMLQSIKEGVVAVDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEECC
RGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEEITIKDRL
CCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECEE
LLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRLDGLVNYADALRERSHEFMNKLHVILG
EEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLHLKSYKQLEDYILKTANNYQEEIGSLLGKIKSPVIAGFLISKINRATDLGHTLILNSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
SLLPDSGSEDQVATLITTLGNLIENALEALGPEPGGEISVTLHYRHGWLHCEVNDDGPGI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEEECCCCCCC
APDKIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAVESEPGIFTQFFVQIPWDGER
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCCC
SNR
CCC
>Mature Secondary Structure
MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQISDMTRDGLANKALAVA
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTDMQSLRYSHPEAQRIGQPF
HHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCCHHHCCCCC
KGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQIGVVAIGLELSRVTQQINDSR
CCCHHHHHHCCCCCEEECHHHHHHHHHHHCCHHCCCCCEEEEEEECHHHHHHHHHHCCHH
WSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEISTLFEQRQAMLQSIKEGVVAVDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEECC
RGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEEITIKDRL
CCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECEE
LLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRLDGLVNYADALRERSHEFMNKLHVILG
EEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLHLKSYKQLEDYILKTANNYQEEIGSLLGKIKSPVIAGFLISKINRATDLGHTLILNSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
SLLPDSGSEDQVATLITTLGNLIENALEALGPEPGGEISVTLHYRHGWLHCEVNDDGPGI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEEECCCCCCC
APDKIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAVESEPGIFTQFFVQIPWDGER
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCCCCCC
SNR
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]