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The map label for this gene is mdtN [H]

Identifier: 157163552

GI number: 157163552

Start: 4334280

End: 4335311

Strand: Reverse

Name: mdtN [H]

Synonym: EcHS_A4327

Alternate gene names: 157163552

Gene position: 4335311-4334280 (Counterclockwise)

Preceding gene: 157163553

Following gene: 157163551

Centisome position: 93.36

GC content: 56.1

Gene sequence:

>1032_bases
ATGGAAAGTACGCCGAAAAAAGCTCCTCGCAGTAAATTCCCTGCTCTGTTAGTGGTTGCGTTGGCGCTGGTTGCCCTTGT
TTTCGTTATCTGGCGCGTAGACAGTGCGCCATCAACTAATGACGCTTACGCGTCAGCAGATACCATTGATGTGGTGCCGG
AAGTCAGCGGTCGCATTGTAGAACTGGCGGTCACCGACAACCAGGCAGTCAAACAGGGCGATTTGCTGTTCCGCATTGAC
CCGCGCCCGTACGAAGCCAATCTGGCGAAAGCTGAAGCCTCCCTCGCGGCGCTGGATAAGCAAATTATGCTCACCCAGCG
TAGCGTTGACGCGCAACAGTTTGGTGCCGACTCGGTTAACGCCACGGTAGAAAAAGCCCGTGCCGCCGCGAAACAGGCCA
CAGATACATTACGCCGCACCGAGCCATTACTGAAAGAAGGTTTTGTCTCAGCGGAAGACGTTGACCGTGCGAGGACCGCG
CAGCGCGCTGCAGAAGCCGATCTTAATGCTGTATTGTTGCAGGCGCAGTCAGCCGCCAGCGCCGTCAGCGGCGTGGATGC
ATTAGTTGCCCAGCGTGCGGCGGTCGAAGCGGATATTGCCCTGACCAAACTGCATCTGGAAATGGCGACCGTTCGCGCGC
CGTTTGATGGCCGGGTCATTTCCCTCAAAACCTCCGTCGGGCAGTTTGCATCTACGATGCGCCCTATTTTTACCCTAATC
GATACTCGTCACTGGTACGTGATCGCCAACTTCCGCGAAACCGATCTGAAAAATATTCGCTCAGGTACACCCGCAACGAT
TCGCCTGATGAGTGACAGCGGCAAAACCTTCGAGGGTAAAGTGGATTCGATTGGCTACGGCGTGCTACCGGATGACGGCG
GCCTGGTGCTGGGCGGCCTGCCGAAAGTTTCTCGCTCTATTAACTGGGTCCGCGTTGCCCAGCGTTTTCCGGTCAAAATC
ATGGTCGATAAACCTGACCCGGAAATGTTCCGCATCGGCGCTTCGGCAGTCGCTAATCTTGAGCCGCAATAA

Upstream 100 bases:

>100_bases
TATCAATCTGGCATTTGTCGGAATTATTTATACCGCCCTTTTTGCTCTCTACGCCATGCTGTTCTGGCTGGCATTTTTCT
AATCTATTTGAGACGCGCTC

Downstream 100 bases:

>100_bases
TGAGCGCGCTCAACTCCCTGCCATTACCGGTGGTCAGGCTGCTGGCGTTCTTTCATGAAGAGTTAAGCGAGCGGCGACCC
GGCCGCGTGCCGCAGATCGT

Product: multidrug resistance protein MdtN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRID
PRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASTMRPIFTLI
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKI
MVDKPDPEMFRIGASAVANLEPQ

Sequences:

>Translated_343_residues
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRID
PRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASTMRPIFTLI
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKI
MVDKPDPEMFRIGASAVANLEPQ
>Mature_343_residues
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRID
PRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASTMRPIFTLI
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKI
MVDKPDPEMFRIGASAVANLEPQ

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790519, Length=343, Percent_Identity=99.7084548104956, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI87081951, Length=318, Percent_Identity=33.0188679245283, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789637, Length=337, Percent_Identity=29.080118694362, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1788711, Length=352, Percent_Identity=27.8409090909091, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789041, Length=333, Percent_Identity=29.1291291291291, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1790012, Length=258, Percent_Identity=29.4573643410853, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790024, Length=254, Percent_Identity=26.7716535433071, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 36939; Mature: 36939

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIV
CCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCEE
ELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVN
EEEECCCCHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHH
ATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADLNAVLLQAQSAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHEEEHHHHHH
AVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASTMRPIFTLI
HHHHHHHHHHHHHHHHHHHHHHHHEEEHHHEECCCCCEEEEEECCHHHHHHHHHHHHHHH
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGL
CCCEEEEEEECCHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCEEEECC
PKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ
HHHHHCCHHEEHHHHCCEEEEEECCCCHHEEECHHHHCCCCCC
>Mature Secondary Structure
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIV
CCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCEE
ELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVN
EEEECCCCHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHH
ATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADLNAVLLQAQSAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHEEEHHHHHH
AVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASTMRPIFTLI
HHHHHHHHHHHHHHHHHHHHHHHHEEEHHHEECCCCCEEEEEECCHHHHHHHHHHHHHHH
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGL
CCCEEEEEEECCHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCEEEECC
PKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ
HHHHHCCHHEEHHHHCCEEEEEECCCCHHEEECHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]