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The map label for this gene is nusG

Identifier: 157163450

GI number: 157163450

Start: 4207325

End: 4207870

Strand: Direct

Name: nusG

Synonym: EcHS_A4215

Alternate gene names: 157163450

Gene position: 4207325-4207870 (Clockwise)

Preceding gene: 157163449

Following gene: 157163451

Centisome position: 90.61

GC content: 51.65

Gene sequence:

>546_bases
ATGTCTGAAGCTCCTAAAAAGCGCTGGTACGTCGTTCAGGCGTTTTCCGGTTTTGAAGGCCGCGTAGCAACGTCGCTGCG
TGAGCATATCAAATTACACAACATGGAAGATTTGTTTGGTGAAGTCATGGTACCAACCGAAGAAGTGGTTGAAATCCGTG
GCGGTCAGCGTCGCAAAAGCGAACGTAAATTCTTCCCTGGCTACGTCCTCGTTCAGATGGTGATGAACGACGCGAGCTGG
CACCTGGTGCGCAGCGTACCGCGTGTGATGGGCTTCATCGGCGGTACTTCCGATCGTCCTGCGCCAATCAGCGATAAAGA
AGTCGATGCGATTATGAACCGCCTGCAGCAGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGG
TCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTG
TCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAA

Upstream 100 bases:

>100_bases
ATTGTGGCTGCGGTTACCGCAGTAATGTCACTGATCCTGTGGGGACTGGATGGTATTCTGGTTCGCCTGGTATCCTTTAT
CACTGGCCTGAGGTTCTGAG

Downstream 100 bases:

>100_bases
CCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT
GGGCCTTGCCCGTAAAACGA

Product: transcription antitermination protein NusG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMVPTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASW
HLVRSVPRVMGFIGGTSDRPAPISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
SVSIFGRATPVELDFSQVEKA

Sequences:

>Translated_181_residues
MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMVPTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASW
HLVRSVPRVMGFIGGTSDRPAPISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
SVSIFGRATPVELDFSQVEKA
>Mature_180_residues
SEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMVPTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWH
LVRSVPRVMGFIGGTSDRPAPISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKVS
VSIFGRATPVELDFSQVEKA

Specific function: Influences transcription termination and antitermination. Acts as a component of the transcription complex, and interacts with the termination factor rho and RNA polymerase

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KOW domain

Homologues:

Organism=Escherichia coli, GI1790414, Length=181, Percent_Identity=100, Blast_Score=368, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUSG_ECO57 (P0AFG1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A98242
- PIR:   F86089
- RefSeq:   NP_290613.1
- RefSeq:   NP_312932.1
- ProteinModelPortal:   P0AFG1
- SMR:   P0AFG1
- MINT:   MINT-1218189
- EnsemblBacteria:   EBESCT00000024851
- EnsemblBacteria:   EBESCT00000059314
- GeneID:   914947
- GeneID:   960148
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5555
- KEGG:   ecs:ECs4905
- GeneTree:   EBGT00050000011221
- HOGENOM:   HBG751162
- OMA:   VASGCEK
- ProtClustDB:   PRK05609
- BioCyc:   ECOL83334:ECS4905-MONOMER
- InterPro:   IPR005824
- InterPro:   IPR001062
- InterPro:   IPR015869
- InterPro:   IPR006645
- InterPro:   IPR014722
- InterPro:   IPR008991
- Gene3D:   G3DSA:2.30.30.30
- Gene3D:   G3DSA:3.30.70.940
- PRINTS:   PR00338
- SMART:   SM00739
- SMART:   SM00738
- TIGRFAMs:   TIGR00922

Pfam domain/function: PF00467 KOW; PF02357 NusG; SSF82679 Transcrpt_antiterm_NusG_N; SSF50104 Transl_SH3_like

EC number: NA

Molecular weight: Translated: 20532; Mature: 20401

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS01014 NUSG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMVPTEEVVEIRGGQRRKS
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCCC
ERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAPISDKEVDAIMNRLQQVGD
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
KPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKVSVSIFGRATPVELDFSQVEK
CCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCHHEEEEEEEECCCCCCEECHHHHCC
A
C
>Mature Secondary Structure 
SEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMVPTEEVVEIRGGQRRKS
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCCC
ERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAPISDKEVDAIMNRLQQVGD
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
KPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKVSVSIFGRATPVELDFSQVEK
CCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCHHEEEEEEEECCCCCCEECHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796