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The map label for this gene is murI

Identifier: 157163444

GI number: 157163444

Start: 4195094

End: 4195951

Strand: Direct

Name: murI

Synonym: EcHS_A4201

Alternate gene names: 157163444

Gene position: 4195094-4195951 (Clockwise)

Preceding gene: 157163443

Following gene: 157163445

Centisome position: 90.34

GC content: 51.28

Gene sequence:

>858_bases
ATGGCTACCAAACTGCAGGACGGGAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTT
TGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCG
ATAACGTCGCTTTTCCGTATGGCGAAAAAAGCGAAGAGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCAGTGCAA
GAACGTTATCCCCTTGCGCTGGCTGTGGTCGCTTGCAACACCGCCAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTT
CGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGG
CAACCCGCGGAACTGTTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTG
GGCTCGGCAGAGATGGTTGAGTTGGCTGAAGCGAAGCTGCATGGCGAAGATGTTTCTCTGGATGCACTAAAACGTATCCT
ACGCCCGTGGTTAAGAATGAAAGAGCCGCCAGATACCGTTGTATTGGGTTGCACCCATTTCCCTCTACTACAAGAAGAAC
TCTTACAGGTGCTGCCAGAGGGAACCCGGCTGGTGGATTCTGGCGCAGCGATTGCTCGCAGAACGGCCTGGCTGTTAGAA
CATGAAGCTCCGGATGCAAAATCTGCCGATGCGAATATTGCCTTTTGTATGGCAATGACGCCAGAAGCTGAACAATTATT
GCCCGTTTTACAGCGTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGA

Upstream 100 bases:

>100_bases
GTGAGCTTGTGGGATCTTGCGGTTGCGTATCCGGTCACCTCTCACCTGACAGTTCGTGGTAAAATAGCCAACCTGTTCGA
CAAAGATTATGAGACAGTCT

Downstream 100 bases:

>100_bases
TTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCAC
CACTGACACGGAACAACGGC

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQ
ERYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARRTAWLLE
HEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFETLEKLAVLG

Sequences:

>Translated_285_residues
MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQ
ERYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARRTAWLLE
HEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFETLEKLAVLG
>Mature_284_residues
ATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQE
RYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEMLG
SAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARRTAWLLEH
EAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFETLEKLAVLG

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=285, Percent_Identity=99.2982456140351, Blast_Score=574, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_ECO24 (A7ZUI7)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001465466.1
- ProteinModelPortal:   A7ZUI7
- SMR:   A7ZUI7
- STRING:   A7ZUI7
- EnsemblBacteria:   EBESCT00000020746
- GeneID:   5587187
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4508
- eggNOG:   COG0796
- GeneTree:   EBGT00050000010644
- HOGENOM:   HBG645102
- OMA:   ARTNAMI
- ProtClustDB:   PRK00865
- BioCyc:   ECOL331111:ECE24377A_4508-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 31132; Mature: 31001

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPY
CCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHEEEEECCEECCC
GEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKP
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCCEEECCCCCCH
AARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEMLGSAEMVELAEAKLHGEDVSL
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCH
DALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARRTAWLLE
HHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHH
HEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFETLEKLAVLG
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
ATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPY
CCCCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHEEEEECCEECCC
GEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKP
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCCEEECCCCCCH
AARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEMLGSAEMVELAEAKLHGEDVSL
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCH
DALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARRTAWLLE
HHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHH
HEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFETLEKLAVLG
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA