Click here to switch to the map view.

The map label for this gene is metL

Identifier: 157163417

GI number: 157163417

Start: 4158468

End: 4160900

Strand: Direct

Name: metL

Synonym: EcHS_A4174

Alternate gene names: 157163417

Gene position: 4158468-4160900 (Clockwise)

Preceding gene: 157163416

Following gene: 157163418

Centisome position: 89.55

GC content: 58.28

Gene sequence:

>2433_bases
ATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTG
TTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCA
CTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTGCGTCGC
TATCAGTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGATCTGGA
ACGCCTGGCGGCGTTGCTCGACAGTGGTATTAACGATGCGGTGTATGCGGAAGTGGTGGGCCACGGTGAAGTGTGGTCGG
CGCGCCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAA
CGCGCCGCACAACCGCAGGTTGATGAAGGGCTTTCTTACCCGTTGCTGCAACAGCTGCTGGTGCAACATCCGGGCAAACG
TCTGGTGGTGACCGGATTTATCAGCCGCAACAACGCCGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCG
CGACACAAATCGGTGCGCTGGCGGGTGTTTCTCGCGTAACCATCTGGAGCGACGTCGCCGGGGTATACAGTGCCGACCCG
CGTAAAGTGAAAGATGCCTGCCTGCTGCCGTTGCTGCGTCTGGATGAGGCCAGCGAACTGGCGCGCCTGGCGGCTCCCGT
TCTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAG
GTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATT
GAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACG
CCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACCTCAGAAGTGGCCGACAGTGCGCTGA
AAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGC
GTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGA
TGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCC
GCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAG
CAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTA
TGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGT
GGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGAT
TTCGCCAGCCACGGTTTCCACGTTATCAGCGCCAACAAACTGGCGGGAGCCAGCGACAGCAATAAATATCGCCAGATCCA
CGACGCCTTCGAAAAAACCGGGCGTCACTGGCTGTACAATGCCACCGTCGGTGCGGGCTTGCCGATCAACCACACCGTGC
GCGATCTGATCGACAGCGGCGATACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACGCTCTCCTGGCTGTTCCTGCAA
TTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACCGAACCTGACCCGCGTGACGA
TCTCTCTGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCAGGTTACAACATCGAACCGGATCAGGTAC
GTGTGGAATCGCTGGTGCCTGCTCATTGCGAAGGCGGCAGCATCGACCATTTCTTTGAAAATGGCGATGAACTGAACGAG
CAGATGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAA
AGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGCTGCCGTGCGATAACGTCTTTGCCATCG
AAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAG
TCGGATATCAACCGGCTGGCACAGTTGTTGTAA

Upstream 100 bases:

>100_bases
TCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGG
GCTGCAAACAAGGGGTAAAA

Downstream 100 bases:

>100_bases
TTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATGTATTGAATTATCATAGGAT
TAGGCCGGATTAAGCGTTTA

Product: bifunctional aspartate kinase II/homoserine dehydrogenase II

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRR
YQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLI
EFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAG
VTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE
QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLD
FASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQ
FDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNE
QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQ
SDINRLAQLL

Sequences:

>Translated_810_residues
MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRR
YQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLI
EFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAG
VTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE
QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLD
FASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQ
FDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNE
QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQ
SDINRLAQLL
>Mature_809_residues
SVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRY
QCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAER
AAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADPR
KVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIE
FQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAGV
TRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFAREQ
STLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDF
ASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQF
DGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQ
MVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQS
DINRLAQLL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1790376, Length=810, Percent_Identity=100, Blast_Score=1647, Evalue=0.0,
Organism=Escherichia coli, GI1786183, Length=821, Percent_Identity=30.694275274056, Blast_Score=334, Evalue=1e-92,
Organism=Escherichia coli, GI1790455, Length=288, Percent_Identity=31.25, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322599, Length=370, Percent_Identity=35.1351351351351, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320893, Length=491, Percent_Identity=23.2179226069246, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AK2H_ECOLI (P00562)

Other databases:

- EMBL:   V00305
- EMBL:   L19201
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S40883
- RefSeq:   AP_003869.1
- RefSeq:   NP_418375.1
- ProteinModelPortal:   P00562
- SMR:   P00562
- DIP:   DIP-10197N
- STRING:   P00562
- EnsemblBacteria:   EBESCT00000004538
- EnsemblBacteria:   EBESCT00000017963
- GeneID:   948433
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3911
- KEGG:   eco:b3940
- EchoBASE:   EB0585
- EcoGene:   EG10590
- eggNOG:   COG0527
- GeneTree:   EBGT00050000009086
- HOGENOM:   HBG298754
- OMA:   GRDMQRK
- ProtClustDB:   PRK09466
- BioCyc:   EcoCyc:ASPKINIIHOMOSERDEHYDROGII-MONOMER
- BioCyc:   MetaCyc:ASPKINIIHOMOSERDEHYDROGII-MONOMER
- Genevestigator:   P00562
- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.1160.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000727
- TIGRFAMs:   TIGR00657

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3; SSF53633 Aa_kinase

EC number: =2.7.2.4; =1.1.1.3

Molecular weight: Translated: 88889; Mature: 88758

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00324 ASPARTOKINASE; PS01042 HOMOSER_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINW
CCCCHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
LKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDA
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAERAAQPQVDEGLSYPLLQQLL
HHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHH
VQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
HHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCHHEEHHHHHHHHCCCCC
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIER
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHH
VLASGTGARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLL
HHHCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
QFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAGVTRNPLHCHRFWQQLKGQPV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCE
EFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE
EEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPY
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCEEEEEECCCC
DDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYN
CCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
ATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLT
ECCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCCCC
EPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEEHHHCCCCCCCCCCCHHHHCCCHHHHH
QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHCCCCCHHHCCCCCEEEECCCEECC
NPLVIRGPGAGRDVTAGAIQSDINRLAQLL
CCEEEECCCCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINW
CCCHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
LKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDA
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAERAAQPQVDEGLSYPLLQQLL
HHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHH
VQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
HHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCHHEEHHHHHHHHCCCCC
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIER
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHH
VLASGTGARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLL
HHHCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
QFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAGVTRNPLHCHRFWQQLKGQPV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCE
EFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE
EEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPY
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCEEEEEECCCC
DDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYN
CCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
ATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLT
ECCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCCCC
EPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEEHHHCCCCCCCCCCCHHHHCCCHHHHH
QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHCCCCCHHHCCCCCEEEECCCEECC
NPLVIRGPGAGRDVTAGAIQSDINRLAQLL
CCEEEECCCCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503