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The map label for this gene is frvA [H]

Identifier: 157163372

GI number: 157163372

Start: 4120570

End: 4121016

Strand: Reverse

Name: frvA [H]

Synonym: EcHS_A4128

Alternate gene names: 157163372

Gene position: 4121016-4120570 (Counterclockwise)

Preceding gene: 157163373

Following gene: 157163371

Centisome position: 88.75

GC content: 49.89

Gene sequence:

>447_bases
ATGGCAGCTCTTACTGCAAGCTGTATTGACCTGAATATTCAGGGCAATGGCGCTTATTCCGTTCTGAAGCAGTTGGCGAC
AATAGCGTTACAAAACGGTTTTATCACCGACTCACACCAGTTCCTGCAAACCCTGCTGCTGCGCGAAAAAATGCACTCTA
CTGAATTTGGTTCCGGTGTCGCCGTGCCGCACGGTAAAAGCGCCTGCGTTAAACAACCGTTCGTATTATTCGCCCGCAAA
GCGCAGGCTATTGACTGGAAAGCCAGCGATGGCGAAGACGTGAATTGCTGGATCTGCCTCGGCGTGCCGCAAAGCGGCGA
AGAGGATCAGGTCAAAATCATCGGCACACTGTGTCGCAAAATTATTCACAAGGAATTTATTCATCAACTGCAACAGGGCG
ATACCGACCAGGTGCTTGCCTTGTTAAATCAAACCCTCAGCTCATAA

Upstream 100 bases:

>100_bases
CTCTGTCTCAAATTGTCATTGTTTACCGACATATCGCCTCTCATCACTTAATCGCTCCACCATTACAATTGATTAAAATA
TATTTTATGGAGTCCGATTA

Downstream 100 bases:

>100_bases
GGAAGTGGCGATGGAGTCATCCTTACGTATTGTCGCGATCACCAACTGCCCCGCCGGGATCGCTCACACCTACATGGTGG
CGGAAGCCCTGGAACAGAAA

Product: putative fructose-like phosphotransferase system subunit EIIA

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system fructose-like EIIA component [H]

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLREKMHSTEFGSGVAVPHGKSACVKQPFVLFARK
AQAIDWKASDGEDVNCWICLGVPQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS

Sequences:

>Translated_148_residues
MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLREKMHSTEFGSGVAVPHGKSACVKQPFVLFARK
AQAIDWKASDGEDVNCWICLGVPQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS
>Mature_147_residues
AALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLREKMHSTEFGSGVAVPHGKSACVKQPFVLFARKA
QAIDWKASDGEDVNCWICLGVPQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367327, Length=148, Percent_Identity=99.3243243243243, Blast_Score=301, Evalue=9e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR004715 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16166; Mature: 16035

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLREKMHSTEFGSGV
CCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
AVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGVPQSGEEDQVKIIGTLCRK
CCCCCCCHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
IIHKEFIHQLQQGDTDQVLALLNQTLSS
HHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQTLLLREKMHSTEFGSGV
CCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
AVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGVPQSGEEDQVKIIGTLCRK
CCCCCCCHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
IIHKEFIHQLQQGDTDQVLALLNQTLSS
HHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8396120; 8346018; 9278503; 8019415 [H]