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The map label for this gene is glnG [H]

Identifier: 157163340

GI number: 157163340

Start: 4084008

End: 4085417

Strand: Reverse

Name: glnG [H]

Synonym: EcHS_A4095

Alternate gene names: 157163340

Gene position: 4085417-4084008 (Counterclockwise)

Preceding gene: 157163341

Following gene: 157163339

Centisome position: 87.98

GC content: 56.67

Gene sequence:

>1410_bases
ATGCAACGAGGGATAGTCTGGGTAGTCGATGACGATAGTTCCATCCGTTGGGTGCTTGAACGTGCGCTCGCTGGAGCGGG
TTTAACCTGTACGACATTTGAGAACGGCGCGGAAGTACTGGAGGCGCTGGCGAGCAAAACGCCGGATGTGCTGCTTTCAG
ATATCCGTATGCCGGGAATGGACGGGCTGGCGCTGCTCAAGCAGATTAAACAGCGCCATCCAATGCTTCCGGTCATCATT
ATGACCGCACATTCCGATCTGGATGCTGCCGTCAGCGCCTATCAACAAGGGGCGTTTGATTATCTGCCCAAACCGTTTGA
TATCGACGAAGCAGTGGCGCTGGTTGAGCGCGCTATCAGTCATTACCAGGAACAGCAGCAGCCGCGTAATATTCAGCTTA
ACGGCCCAACGACCGATATCATCGGCGAAGCGCCAGCCATGCAGGACGTGTTCCGTATTATCGGTCGGCTTTCGCGTTCT
TCTATTAGCGTGCTGATTAACGGCGAATCCGGCACCGGTAAAGAACTGGTCGCTCATGCCCTGCATCGCCACAGTCCGCG
CGCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGA
AAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTGAACAGGCCGATGGCGGTACATTATTCCTCGACGAA
ATTGGTGATATGCCGCTGGATGTGCAGACGCGTTTGCTGCGCGTGCTGGCAGACGGTCAGTTTTACCGCGTTGGCGGCTA
TGCGCCGGTGAAAGTGGATGTGCGGATTATCGCTGCCACTCACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCC
GTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGT
CTGGCGCGCCATTTTTTACAGGTTGCCGCGCACGAACTGGGCGTAGAAGCGAAGTTACTGCATCCGGAAACCGAAGCTGC
TCTGACGCGTCTGGCGTGGCCAGGCAACGTGCGCCAGCTGGAAAACACCTGCCGCTGGCTAACGGTGATGGCCGCCGGGC
AGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGAC
AGTTGGGCGACGCTTCTTGCGCAGTGGGCAGACAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCC
AGAGCTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCT
GGGGCCGCAACACCCTGACGCGTAAGTTAAAAGAGCTGGGGATGGAGTGA

Upstream 100 bases:

>100_bases
ATTTGATTGATCAGCATTCAGGCAAAATTGAATTTACCAGTTGGCCAGGGCATACCGAGTTCTCGGTTTACCTGCCTATC
AGGAAATAAAGGTGACGTTT

Downstream 100 bases:

>100_bases
TTCACAGCTTGTGTGTAAAGATTGATTATTGAGCGCAAATTGCTGGTATTTTACGCTTTACTGTTCCGATAAGTTCAGTA
TGATCTTGCCCGGAAAACGG

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPR
LARHFLQVAAHELGVEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPD
SWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Sequences:

>Translated_469_residues
MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPR
LARHFLQVAAHELGVEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPD
SWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
>Mature_469_residues
MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPR
LARHFLQVAAHELGVEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPD
SWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=99.5735607675906, Blast_Score=952, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=42.9487179487179, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1790437, Length=462, Percent_Identity=41.991341991342, Blast_Score=344, Evalue=9e-96,
Organism=Escherichia coli, GI1788905, Length=464, Percent_Identity=38.1465517241379, Blast_Score=297, Evalue=8e-82,
Organism=Escherichia coli, GI87082117, Length=364, Percent_Identity=42.032967032967, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1789233, Length=329, Percent_Identity=43.161094224924, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1786524, Length=345, Percent_Identity=43.4782608695652, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789087, Length=374, Percent_Identity=39.8395721925134, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1787583, Length=331, Percent_Identity=40.4833836858006, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI87082152, Length=336, Percent_Identity=40.4761904761905, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87081872, Length=333, Percent_Identity=39.9399399399399, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI87081858, Length=312, Percent_Identity=32.0512820512821, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789828, Length=238, Percent_Identity=35.2941176470588, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790860, Length=146, Percent_Identity=32.1917808219178, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=30.0813008130081, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786911, Length=123, Percent_Identity=31.7073170731707, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787487, Length=100, Percent_Identity=33, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789402, Length=111, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI145693157, Length=121, Percent_Identity=31.404958677686, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787375, Length=108, Percent_Identity=31.4814814814815, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52250; Mature: 52250

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHA
HHHHHCCCCCEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHHH
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHHH
LNVIRVHLPPLRERREDIPRLARHFLQVAAHELGVEAKLLHPETEAALTRLAWPGNVRQL
HHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCHHHH
ENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPDSWATLLAQWADRALRSGHQN
HHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHA
HHHHHCCCCCEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHHH
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHHH
LNVIRVHLPPLRERREDIPRLARHFLQVAAHELGVEAKLLHPETEAALTRLAWPGNVRQL
HHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCHHHH
ENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPDSWATLLAQWADRALRSGHQN
HHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]