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The map label for this gene is fadA [H]

Identifier: 157163319

GI number: 157163319

Start: 4057869

End: 4059032

Strand: Reverse

Name: fadA [H]

Synonym: EcHS_A4068

Alternate gene names: 157163319

Gene position: 4059032-4057869 (Counterclockwise)

Preceding gene: 157163320

Following gene: 157163316

Centisome position: 87.41

GC content: 58.25

Gene sequence:

>1164_bases
ATGGAACAGGTTGTCATTGTCGATGCAATTCGCACCCCGATGGGCCGTTCGAAGGGCGGTGCTTTTCGTAACGTGCGTGC
AGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTT
ACTGGGGTTGTGTGCAGCAGACGCTGGAGCAGGGTTTTAACATCGCCCGTAACGCGGCGCTGCTGGCAGAAGTACCACAC
TCTGTCCCGGCGGTTACCGTCAATCGCTTGTGTGGTTCATCCATGCAGGCACTGCATGACGCAGCACGGATGATTATGAC
CGGCGATGCGCAGGCATGTCTGGTTGGCGGCGTGGAGCATATGGGCCATGTGCCGATGAGTCACGGCGTCGATTTTCACC
CCGGCCTGAGCCGCAATGTCGCCAAAGCGGCGGGCATGATGGGCTTAACAGCAGAAATGCTGGCGCGTATGCACGGTATC
AGCCGTGAAATGCAGGATGCCTTTGCCGCGCGATCGCACGCTCGTGCCTGGGCCGCCACGCAGTCGGCCGCATTTAAAAA
TGAAATCATCCCGACCGGTGGTCACGATGCCGACGGCGTCCTGAAGCAGTTTAATTACGACGAAGTGATTCGCCCGGAAA
CCACCGTGGAAGCCCTCGCCACGCTGCGTCCGGCGTTTGATCCAGTAAACGGTACGGTAACGGCGGGCACATCTTCTGCA
CTTTCCGATGGCGCAGCTGCCATGCTGGTGATGAGTGAAAGCCGCGCCCATGAATTAGGTCTTAAGCCGCGCGCTCGTGT
GCGTTCGATGGCGGTCGTTGGTTGTGACCCATCGATTATGGGTTACGGCCCGGTTCCGGCCTCAAAGCTGGCGCTGAAAA
AAGCGGGGCTTTCTGCCAGCGATATCGGCGTGTTTGAGATGAACGAAGCCTTTGCCGCGCAGATCCTGCCGTGCATTAAA
GATCTCGGGCTAATGGAGCAGATTGACGAGAAGATCAACCTCAATGGTGGCGCGATCGCGCTGGGTCATCCGCTGGGTTG
TTCCGGTGCGCGTATCAGCACCACGCTGCTGAATCTGATGGAGCGCAAAGACGTTCAGTTTGGTCTGGCGACGATGTGTA
TCGGTCTGGGTCAGGGTATTGCGACGGTGTTTGAGCGGGTTTAA

Upstream 100 bases:

>100_bases
GGAAGGTCTGCGTAATAAAGCGCGTCATAACGAACCGTACTATCCTCCGGTTGAGCCAGCCCGTCCGGTTGGCGACCTGA
AAACGGCTTAAGGAGTCACA

Downstream 100 bases:

>100_bases
CCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACAGATCCGGCACCAATGTAGGCCTGATAAGACGCGCCAGCG
TCGCATCAGGCATCGTGCAC

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVNGTVTAGTSSA
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV

Sequences:

>Translated_387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVNGTVTAGTSSA
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV
>Mature_387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVNGTVTAGTSSA
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=42.9292929292929, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=38.4810126582278, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI148539872, Length=400, Percent_Identity=38.75, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=34.032634032634, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=34.5771144278607, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI194353979, Length=393, Percent_Identity=31.5521628498728, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI48994986, Length=387, Percent_Identity=99.4832041343669, Blast_Score=786, Evalue=0.0,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=45.4320987654321, Blast_Score=317, Evalue=6e-88,
Organism=Escherichia coli, GI1788554, Length=405, Percent_Identity=43.2098765432099, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=40.8977556109726, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=35.6973995271868, Blast_Score=191, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=41.3965087281795, Blast_Score=264, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI17535921, Length=403, Percent_Identity=33.7468982630273, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=33.7209302325581, Blast_Score=190, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=395, Percent_Identity=31.3924050632911, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=388, Percent_Identity=27.319587628866, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322031, Length=391, Percent_Identity=39.6419437340153, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6325229, Length=408, Percent_Identity=34.8039215686275, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=40.2985074626866, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17648125, Length=402, Percent_Identity=39.5522388059701, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=34.032634032634, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24640423, Length=406, Percent_Identity=31.7733990147783, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40865; Mature: 40865

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHCHHH
MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVNGTVTAGTSSA
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSAS
HCCCCEEEEEEECCHHHHCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERKDVQFGLATMCIGLGQGIATVFERV
HHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHCHHH
MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVNGTVTAGTSSA
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSAS
HCCCCEEEEEEECCHHHHCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERKDVQFGLATMCIGLGQGIATVFERV
HHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA