| Definition | Escherichia coli HS, complete genome. |
|---|---|
| Accession | NC_009800 |
| Length | 4,643,538 |
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The map label for this gene is metE [H]
Identifier: 157163305
GI number: 157163305
Start: 4043411
End: 4045672
Strand: Direct
Name: metE [H]
Synonym: EcHS_A4053
Alternate gene names: 157163305
Gene position: 4043411-4045672 (Clockwise)
Preceding gene: 157163303
Following gene: 157163307
Centisome position: 87.08
GC content: 56.01
Gene sequence:
>2262_bases ATGACAATTCTTAATCACACCCTCGGTTTCCCTCGCGTTGGCCTGCGTCGCGAGCTGAAAAAAGCGCAAGAAAGTTATTG GGCGGGGAACTCCACGCGTGAAGAACTGCTGACAGTAGGGCGTGAACTGCGTGCTCGTCACTGGGATCAACAAAAGCAAG CGGGTATCGACCTGCTGCCGGTGGGCGATTTTGCCTGGTACGATCATGTACTGACCACCAGTTTGCTGCTGGGTAATGTT CCGCCACGTCATCAGAACAAAGACGGTTCGGTAGATATCGACACCCTGTTCCGTATTGGTCGTGGACGTGCGCCGACTGG CGAACCTGCGGCGGCAGCGGAAATGACCAAATGGTTTAACACCAACTATCACTACATGGTGCCGGAGTTCGTTAAAGGCC AACAGTTCAAACTGACCTGGACGCAGCTGCTGGAGGAAGTGGACGAGGCGCTGGCGCTGGGCCACAACGTTAAGCCTGTG CTGCTGGGGCCGGTTACTTACCTGTGGCTGGGGAAAGTGAAAGGTGAACAGTTTGACCGCCTTAGCCTGCTGAACGACAT TCTGCCGGTTTATCAGCAAGTGCTGGCAGAACTGGCGAAACGCGGCATCGAGTGGGTACAGATTGATGAACCCGCGCTGG TACTGGAACTACCACAGGCGTGGCTGGACGCATACAAACCCGCTTACGACGCGCTCCAGGGACAGGTGAAACTGCTGCTG ACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGT ACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTC GTAACGTCTGGCGCGCCGATCTTACCGAGAAATATGCGCAAATTAAGGACATTGTCGGCAAACGTGATTTGTGGGTGGCA TCTTCCTGCTCGTTGCTGCACAGCCCCATCGACCTGAGCGTGGAAACGCGTCTTGATGCAGAAGTGAAAAGCTGGTTTGC CTTCGCCCTACAAAAATGCCATGAACTGGCACTGCTGCGCGATGCGCTGAACAGTGGTGACACGGCAGCTCTGGCAGAGT GGAGCGCCCCGATTCAGGCACGTCGTCACTCTACCCGCGTACATAATCCGGCGGTAGAAAAGCGTCTGGCGGCGATCACC GCCCAGGACAGCCAGCGTGCGAATGTCTATGAAGTGCGTGCTGAAGCCCAGCGTGCGCGTTTTAAACTGCCAGCGTGGCC GACCACCACGATTGGTTCCTTCCCGCAAACCACGGAAATTCGTACCCTGCGTCTGGATTTCAAAAAGGGCAATCTCGACG CCAACAACTACCGCACGGGCATTGCGGAACATATCAAGCAGGCCATTGTTGAGCAGGAACGTTTGGGACTGGATGTGCTG GTACATGGCGAGGCCGAGCGTAATGACATGGTGGAATACTTTGGCGAGCACCTCGACGGATTTGTCTTTACGCAAAACGG TTGGGTACAGAGCTACGGTTCCCGCTGCGTGAAGCCACCGATTGTCATTGGTGACATTAGCCGCCCGGCACCGATTACCG TGGAGTGGGCGAAGTATGCGCAATCGCTGACCGACAAACCGGTGAAAGGGATGCTGACGGGGCCGGTGACCATACTCTGC TGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAAACCATCGCCAAACAGATTGCGCTGGCGCTGCGTGATGAAGTGGCCGA TCTGGAAGCCGCTGGAATTGGCATCATCCAGATTGACGAACCGGCGCTGCGCGAAGGTTTACCGCTGCGTCGTAGCGACT GGGATGCGTATCTCCAGTGGGGCGTAGAGGCCTTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACT CACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTC GCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTC ACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTG TGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGC GCAGAACTTGCGTCGGGGGTAA
Upstream 100 bases:
>100_bases GCATGAATAATTTGCGCTTGAGGAATATACAGTAACCGCCAATTATGGATGTGTAAACATCTGGACGGCTAAATACTAAA AATTCCATAAGGGGCATATA
Downstream 100 bases:
>100_bases AATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCA GCATACGCTGCCAGCCATCT
Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
Products: NA
Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]
Number of amino acids: Translated: 753; Mature: 752
Protein sequence:
>753_residues MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNV PPRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVA SSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAIT AQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILC WSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHT HMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG
Sequences:
>Translated_753_residues MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNV PPRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVA SSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAIT AQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILC WSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHT HMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG >Mature_752_residues TILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVP PRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPVL LGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLLT TYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVAS SCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITA QDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVLV HGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCW SFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTH MCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLW VNPDCGLKTRGWPETRAALANMVQAAQNLRRG
Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]
COG id: COG0620
COG function: function code E; Methionine synthase II (cobalamin-independent)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]
Homologues:
Organism=Escherichia coli, GI2367304, Length=753, Percent_Identity=99.33598937583, Blast_Score=1536, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6320936, Length=761, Percent_Identity=49.1458607095926, Blast_Score=716, Evalue=0.0,
Paralogues:
None
Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013215 - InterPro: IPR006276 - InterPro: IPR002629 [H]
Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]
EC number: =2.1.1.14 [H]
Molecular weight: Translated: 84707; Mature: 84576
Theoretical pI: Translated: 5.65; Mature: 5.65
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 2.1 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 2.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELRARHWDQQKQAGIDLLP CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEE VGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFN CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC TNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPVLLGPVTYLWLGKVKGEQFDR CCCEEECHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCHHHH LSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHCHHHHHHHHHHHHHH TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRAD HHHHCCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHCCHHHHHHCCCCCCCCCCHHH LTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLR HHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHH DALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVRAEAQRAR HHHCCCCCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC FKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL EECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDISRPAPITVEWAKYA EECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCCCEEHHHHHH QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE HHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC PALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDAD HHHHCCCCCCCCCHHHHHHHCCHHEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCC VITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL EEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure TILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELRARHWDQQKQAGIDLLP CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCEEEE VGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFN CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC TNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPVLLGPVTYLWLGKVKGEQFDR CCCEEECHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCHHHH LSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLL HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHCHHHHHHHHHHHHHH TTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRAD HHHHCCCCCCCCCEEECCCCCCEEEEECCCHHHHHHHHHCCHHHHHHCCCCCCCCCCHHH LTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLR HHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEEEHHCCHHHHHHHHHHHHHHHHHHHHH DALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVRAEAQRAR HHHCCCCCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC FKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVL EECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE VHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDISRPAPITVEWAKYA EECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCCCEEHHHHHH QSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDE HHHCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECC PALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDAD HHHHCCCCCCCCCHHHHHHHCCHHEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCC VITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERL EEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE WVNPDCGLKTRGWPETRAALANMVQAAQNLRRG EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA