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The map label for this gene is rhtC [H]

Identifier: 157163299

GI number: 157163299

Start: 4038240

End: 4038854

Strand: Direct

Name: rhtC [H]

Synonym: EcHS_A4047

Alternate gene names: 157163299

Gene position: 4038240-4038854 (Clockwise)

Preceding gene: 157163298

Following gene: 157163301

Centisome position: 86.96

GC content: 51.38

Gene sequence:

>615_bases
ATGTTATTTCTCACCGTCGCCATGGTGCATATTGTGGCGCTTATGAGCCCCGGTCCCGATTTCTTTTTTGTCTCTCAGAC
CGCTGTTAGTCGTTCCCGTAAAGAAGCGATGATGGGCGTGCTGGGCATTACCTGCGGTGTAATGGTTTGGGCTGGGATTG
CGTTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTTGGCGGTGGCCTGTATCTC
TGCTGGATGGGTTACCAGATGCTACGCGGTGCACTGAAAAAAGAGGCTGTATCTGCGCCTGCACCACAGATTGAGCTGGC
GAAAAGCGGGCGCAGTTTTCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGT
TCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACACTGGCG
TGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGG
TTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGA

Upstream 100 bases:

>100_bases
CGCGCATGTTGACGGCGACGACGAAGAGTAGTCAGCGGCATAAAAAAGTGCCAGGATGAAGACTCCGTAAACGTTTCCCC
CGCGAGTCAAATGTATGTTG

Downstream 100 bases:

>100_bases
TGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGAT
GCTAACAGCGCTCCCACCAG

Product: threonine efflux system

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLGITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYL
CWMGYQMLRGALKKEAVSAPAPQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETLA
WFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR

Sequences:

>Translated_204_residues
MLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLGITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYL
CWMGYQMLRGALKKEAVSAPAPQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETLA
WFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR
>Mature_204_residues
MLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLGITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYL
CWMGYQMLRGALKKEAVSAPAPQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETLA
WFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR

Specific function: Conducts the efflux of threonine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994978, Length=204, Percent_Identity=99.5098039215686, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1786522, Length=206, Percent_Identity=28.1553398058252, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788099, Length=197, Percent_Identity=27.9187817258883, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22244; Mature: 22244

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLGITCGVMVWAGIALLGLHL
CHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQIELAKSGRSFLKGLLTN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETLAWFTVVASLFALPQMRRGYQR
CCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKWIDGFAGALFAGFGIHLIISR
HHHHHHHHHHHHHHHHHHHEEEEC
>Mature Secondary Structure
MLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLGITCGVMVWAGIALLGLHL
CHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQIELAKSGRSFLKGLLTN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETLAWFTVVASLFALPQMRRGYQR
CCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKWIDGFAGALFAGFGIHLIISR
HHHHHHHHHHHHHHHHHHHEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]