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The map label for this gene is wecE

Identifier: 157163265

GI number: 157163265

Start: 4003559

End: 4004689

Strand: Direct

Name: wecE

Synonym: EcHS_A4008

Alternate gene names: 157163265

Gene position: 4003559-4004689 (Clockwise)

Preceding gene: 157163264

Following gene: 157163266

Centisome position: 86.22

GC content: 54.55

Gene sequence:

>1131_bases
ATGATTCCATTTAACGCACCGCCGGTGGTGGGAACCGAACTCGACTATATGCAGTCGGCAATGGGTAGCGGCAAACTGTG
TGGCGATGGCGGTTTTACCCGTCGCTGCCAGCAGTGGCTGGAGCAACGTTTTGGCAGCGCCAAAGTGTTACTGACGCCGT
CCTGCACCGCTTCGCTGGAGATGGCGGCGCTGCTGCTCGATATCCAGCCTGGCGATGAAGTGATCATGCCGAGCTACACC
TTTGTCTCCACCGCCAATGCCTTTGTGCTGCGTGGCGCAAAAATCGTTTTTGTGGATGTTCGCCCGGACACCATGAACAT
CGACGAAACGCTGATTGAAGCGGCGATCACCGACAAAACGCGCGTTATCGTGCCGGTCCATTACGCGGGTGTGGCCTGCG
AAATGGACACCATTATGGCGTTGGCGAAAAAGCATAATCTTTTTGTGGTGGAAGATGCCGCTCAGGGCGTGATGTCCACT
TACAAAGGGCGTGCACTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGTGG
TGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTAATCGAACGAGCCGAGATCATCCGTGAAAAGGGCACTAACCGCA
GCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCT
GCGTACCTGTGGGCGCAACTGGAAGCAGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGC
GTTAGCGCCTCTGGCGAAAGCCGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCT
ACATTAAACTGCGGGATATTGATGACCGGAGCGCGTTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTTCAT
TACATTCCGCTGCACGGTTGCCCTGCGGGGGAACACTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAG
CGAGCGCCTGCTGCGCCTGCCGCTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACTTTGTTGAACT
ACTTCTCCTGA

Upstream 100 bases:

>100_bases
CGGGTGGCGACCCAGATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAAAGCACCGCGTA
CTGGTTATACAGGTGATCAC

Downstream 100 bases:

>100_bases
TATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGT
TGCTGGCTGTGTCATTTGGT

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRFGSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYT
FVSTANAFVLRGAKIVFVDVRPDTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
AYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGRIELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFH
YIPLHGCPAGEHFGEFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS

Sequences:

>Translated_376_residues
MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRFGSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYT
FVSTANAFVLRGAKIVFVDVRPDTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
AYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGRIELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFH
YIPLHGCPAGEHFGEFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS
>Mature_376_residues
MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRFGSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYT
FVSTANAFVLRGAKIVFVDVRPDTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
AYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGRIELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFH
YIPLHGCPAGEHFGEFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS

Specific function: Involved in ECA elongation

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family

Homologues:

Organism=Escherichia coli, GI2367285, Length=376, Percent_Identity=100, Blast_Score=787, Evalue=0.0,
Organism=Escherichia coli, GI145693159, Length=389, Percent_Identity=31.1053984575836, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFFA_ECOLI (P27833)

Other databases:

- EMBL:   M87049
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B65183
- RefSeq:   AP_004006.1
- RefSeq:   NP_418238.1
- ProteinModelPortal:   P27833
- SMR:   P27833
- STRING:   P27833
- EnsemblBacteria:   EBESCT00000001698
- EnsemblBacteria:   EBESCT00000014329
- GeneID:   948296
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3765
- KEGG:   eco:b3791
- EchoBASE:   EB1425
- EcoGene:   EG11456
- eggNOG:   COG0399
- GeneTree:   EBGT00050000010722
- HOGENOM:   HBG660897
- OMA:   VHYAGVS
- ProtClustDB:   PRK11706
- BioCyc:   EcoCyc:RFFTRANS-MONOMER
- BioCyc:   MetaCyc:RFFTRANS-MONOMER
- Genevestigator:   P27833
- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF000390
- TIGRFAMs:   TIGR02379

Pfam domain/function: PF01041 DegT_DnrJ_EryC1; SSF53383 PyrdxlP-dep_Trfase_major

EC number: NA

Molecular weight: Translated: 41902; Mature: 41902

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRFGSAKVLLTPSCTASLE
CCCCCCCCCCCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHE
MAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRPDTMNIDETLIEAAITDKT
EEEEEEEECCCCCEEECCCHHHHCCCEEEEECCEEEEEEECCCCCCHHHHHHHHHHCCCC
RVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMSTYKGRALGTIGHIGCFSFHET
EEEEEEEECCEEEHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCEEECHHHHEEEEEECC
KNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
CCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHH
AYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGRIELPSIPDGCVQNAHMFYIKLRDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHCCCEEEEEEEECC
DDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFGEFHGEDRYTTKESERLLRLPLFYNLS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHEEECCEEECCC
PVNQRTVIATLLNYFS
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRFGSAKVLLTPSCTASLE
CCCCCCCCCCCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHE
MAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRPDTMNIDETLIEAAITDKT
EEEEEEEECCCCCEEECCCHHHHCCCEEEEECCEEEEEEECCCCCCHHHHHHHHHHCCCC
RVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMSTYKGRALGTIGHIGCFSFHET
EEEEEEEECCEEEHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCEEECHHHHEEEEEECC
KNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
CCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHH
AYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGRIELPSIPDGCVQNAHMFYIKLRDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHCCCEEEEEEEECC
DDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFGEFHGEDRYTTKESERLLRLPLFYNLS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHEEECCEEECCC
PVNQRTVIATLLNYFS
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065