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The map label for this gene is ilvG [H]

Identifier: 157163243

GI number: 157163243

Start: 3978971

End: 3980617

Strand: Direct

Name: ilvG [H]

Synonym: EcHS_A3985

Alternate gene names: 157163243

Gene position: 3978971-3980617 (Clockwise)

Preceding gene: 157163242

Following gene: 157163244

Centisome position: 85.69

GC content: 54.46

Gene sequence:

>1647_bases
ATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAATACCGTTTTCGGTTATCCGGGTGGCGCAAT
TATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTGTGCCGACATGAACAGGGTGCGGCAATGGCGG
CTATCGGTTATGCCCGTGCTACTGGCAAAACTGGCGTATGTATCGCCACGTCTGGTCCGGGCGCAACCAACCTGATAACC
GGGCTTGCGGACGCACTGTTAGATTCCATCCCCGTTGTTGCCATCACCGGTCAAGTGTCCGCACCGTTTATCGGCACGGA
CGCATTTCAGGAAGTGGATGTCCTGGGATTGTCGCTAGCCTGTACCAAGCACAGCTTCCTGGTGCAGTCGCTGGAAGAGT
TGCCGCGCATCATGGCTGAAGCATTCGACGTTGCCAGCTCAGGTCGTCCTGGTCCGGTTCTGGTCGATATCCCAAAAGAT
ATCCAATTAGCCAGCGGCGACCTGGAACCGTGGTTCACCACCGTTGAAAACGAAGTGACTTTCCCACATGCCGAAGTCGA
GCAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGTGGTGGCGTGGGTATGGCGCAGGCAGTTC
CTGCTTTACGAGAATTTCTCGCTACCACAAAAATGCCTGCCACCTGCACGCTGAAAGGGCTGGGCGCAGTTGAAGCAGAT
TATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGAT
CGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGG
ATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCA
TTACAGCAGCCGTTAAATATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCA
TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAG
ATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTA
GGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGG
TGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCG
ATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGAT
AACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCCTGGCCAACACATCACCCGTAAAGACCAGGTTGAAGCGGC
ACTCAACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGG
TGCCGCCAGGTGCCAGTAATTCAGAAATGTTGGAGAAATTATCATGA

Upstream 100 bases:

>100_bases
GTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGA
TTCAGGACGGGGAACTAACT

Downstream 100 bases:

>100_bases
TGCAACATCAGGTCAATGTATCGGCTCGCTTCAATCCAGAAACCTTAGAACGTGTTTTACGCGTGGTGCGTCATCGTGGT
TTCCACGTCTGCTCAATGAA

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARATGKTGVCIATSGPGATNLIT
GLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLATTKMPATCTLKGLGAVEAD
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPA
LQQPLNINDWQQHCAQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGL
GTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
NPDFLMLASAFGIPGQHITRKDQVEAALNTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS

Sequences:

>Translated_548_residues
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARATGKTGVCIATSGPGATNLIT
GLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLATTKMPATCTLKGLGAVEAD
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPA
LQQPLNINDWQQHCAQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGL
GTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
NPDFLMLASAFGIPGQHITRKDQVEAALNTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS
>Mature_548_residues
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARATGKTGVCIATSGPGATNLIT
GLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLATTKMPATCTLKGLGAVEAD
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPA
LQQPLNINDWQQHCAQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGL
GTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
NPDFLMLASAFGIPGQHITRKDQVEAALNTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=557, Percent_Identity=24.0574506283662, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI21361361, Length=498, Percent_Identity=24.2971887550201, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=46.7391304347826, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=43.8721136767318, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1786717, Length=559, Percent_Identity=30.0536672629696, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1787096, Length=541, Percent_Identity=30.3142329020333, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788716, Length=552, Percent_Identity=27.7173913043478, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=560, Percent_Identity=27.1428571428571, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=560, Percent_Identity=27.1428571428571, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=501, Percent_Identity=24.5508982035928, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=577, Percent_Identity=43.5008665511265, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320816, Length=488, Percent_Identity=22.5409836065574, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922626, Length=559, Percent_Identity=25.7602862254025, Blast_Score=175, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59178; Mature: 59178

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARA
CCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEEHHHHHC
TGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLA
CCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHCEEEH
CTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKDIQLASGDLEPWFTTVENEVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEECCCCCCCHHHCCCCCCC
FPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLATTKMPATCTLKGLGAVEAD
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEM
CCHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
NKLRQAHVALQGDLNALLPALQQPLNINDWQQHCAQLRDEHAWRYDHPGDAIYAPLLLKQ
HHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGLGTMGFGLPAAVGAQVARPND
HCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHCCEEECCCC
TVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
EEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
NPDFLMLASAFGIPGQHITRKDQVEAALNTMLNSDGPYLLHVSIDELENVWPLVPPGASN
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCC
SEMLEKLS
HHHHHHCC
>Mature Secondary Structure
MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARA
CCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCEEEEHHHHHC
TGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLA
CCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHCEEEH
CTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKDIQLASGDLEPWFTTVENEVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEECCCCCCCHHHCCCCCCC
FPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLATTKMPATCTLKGLGAVEAD
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEM
CCHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
NKLRQAHVALQGDLNALLPALQQPLNINDWQQHCAQLRDEHAWRYDHPGDAIYAPLLLKQ
HHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGLGTMGFGLPAAVGAQVARPND
HCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHCCEEECCCC
TVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD
EEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
NPDFLMLASAFGIPGQHITRKDQVEAALNTMLNSDGPYLLHVSIDELENVWPLVPPGASN
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCC
SEMLEKLS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]