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The map label for this gene is bglB [H]

Identifier: 157163201

GI number: 157163201

Start: 3929469

End: 3930881

Strand: Reverse

Name: bglB [H]

Synonym: EcHS_A3935

Alternate gene names: 157163201

Gene position: 3930881-3929469 (Counterclockwise)

Preceding gene: 157163202

Following gene: 157163200

Centisome position: 84.65

GC content: 48.34

Gene sequence:

>1413_bases
ATGAAAGCATTTCCAGAAACATTTCTTTGGGGTGGCGCAACAGCTGCCAATCAGGTGGAAGGTGCCTGGCAGGAAGATGG
CAAAGGGATCTCGACCTCAGATTTACAGCCTCATGGCGTAATGGGAAAAATGGAACCGCGCATCCTGGGGAAAGAGAATA
TCAAAGATGTCGCCATCGATTTTTATCACCGTTACTCGGAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTG
CGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGAAGTCGAACCGAATGAAGCGGGGTTAGCGTTTTACGA
TCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGG
TGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCGATCACTTCGAGCATTACGCCCGCACGGTCTTTACTCGCTAC
CAACATAAAGTGGCGTTATGGCTGACGTTTAATGAAATCAACATGTCGTTACACGCGCCATTCACGGGCGTGGGGCTGGC
AGAAGAGAGTGGCGAGGCGGAAGTTTATCAGGCTATCCACCATCAACTGGTTGCCAGTGCGCGGGCAGTTAAAGCCTGTC
ATAGCCTGCTACCCGAAGCGAAAATCGGCAATATGCTTCTCGGTGGGCTGGTTTACCCCCTCACCTGCCAGCCACAGGAT
ATGTTGCAGGCCATGGAAGAGAACCGGCGCTGGATGTTCTTTGGTGATGTTCAGGCGCGTGGCCAGTATCCCGGCTATAT
GCAGCGTTTCTTCCGCGACCACAATATCACCATTGAGATGACTGAAAGTGACGCAGAAGATTTAAAACATACCGTCGATT
TCATCTCTTTTAGTTATTACATGACTGGTTGTGTTTCCCACGACGAAAGCATTAATAAAAATGCGCAGGGCAACATACTG
AATATGATCCCCAATCCGCATCTGAAAAGTTCAGAGTGGGGGTGGCAAATTGATCCGGTTGGATTACGGGTTCTGTTAAA
TACGCTTTGGGATCGTTATCAAAAACCGTTATTTATTGTCGAGAACGGATTAGGCGCAAAAGACAGCGTTGAAGCGGATG
GTTCGATACAGGACGATTATCGAATTGCCTATTTAAACGATCACCTGGTACAGGTAAATGAAGCGATTGCCGATGGTGTG
TATATTATGGGGTACACCAGTTGGGGGCCAATTGATTTAGTCAGTGCATCTCATTCACAAATGTCTAAGCGCTACGGCTT
TATTTATGTGGATCGTGATGATAATGGCGAAGGAAGCCTCACAAGAACACGCAAGAAAAGCTTCGGATGGTATGCAGAGG
TGATCAAGACGCGGGGGCTGTCATTAAAAAAAATAACCATTAAAGCACCTTAA

Upstream 100 bases:

>100_bases
TAGCGATGATTTTACGGACGTATTACCCCACGGCACGGCGCAGATAAGCGCAGGTGAACCGCTGTTATCCATCATTCGCT
AACGATAAAAGGAGTTAATT

Downstream 100 bases:

>100_bases
TTATCGTCGCATTCAGAACAGTCTGGATGCGATGCGTTAATTCTTTCTTTGCACCATAAAGGGATATTATGTTTAGACGA
AATCTTATTACCTCTGCCAT

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKMEPRILGKENIKDVAIDFYHRYSEDIALFAEMGFTCL
RISIAWARIFPQGDEVEPNEAGLAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLPEAKIGNMLLGGLVYPLTCQPQD
MLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNIL
NMIPNPHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQVNEAIADGV
YIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKKITIKAP

Sequences:

>Translated_470_residues
MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKMEPRILGKENIKDVAIDFYHRYSEDIALFAEMGFTCL
RISIAWARIFPQGDEVEPNEAGLAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLPEAKIGNMLLGGLVYPLTCQPQD
MLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNIL
NMIPNPHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQVNEAIADGV
YIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKKITIKAP
>Mature_470_residues
MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKMEPRILGKENIKDVAIDFYHRYSEDIALFAEMGFTCL
RISIAWARIFPQGDEVEPNEAGLAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLPEAKIGNMLLGGLVYPLTCQPQD
MLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNIL
NMIPNPHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQVNEAIADGV
YIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKKITIKAP

Specific function: Can hydrolyze salicin and arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=482, Percent_Identity=29.4605809128631, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI110681710, Length=495, Percent_Identity=30.1010101010101, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI13273313, Length=483, Percent_Identity=29.399585921325, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI24497614, Length=454, Percent_Identity=30.1762114537445, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI28376633, Length=480, Percent_Identity=27.5, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI2367270, Length=470, Percent_Identity=99.5744680851064, Blast_Score=979, Evalue=0.0,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=54.2194092827004, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI2367174, Length=474, Percent_Identity=50.8438818565401, Blast_Score=469, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17552856, Length=487, Percent_Identity=29.1581108829569, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17539390, Length=480, Percent_Identity=28.3333333333333, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI21356577, Length=490, Percent_Identity=27.3469387755102, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53200; Mature: 53200

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKMEPRILGKENIKDVAID
CCCCCCHHCCCCCCCCHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
FYHRYSEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAGLAFYDRLFDEMAQAGIKP
HHHHHHHHHHHHHHHCCEEEEEEEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
LVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRYQHKVALWLTFNEINMSLHAP
EEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECEEEECC
FTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLPEAKIGNMLLGGLVYPLTCQPQD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCEEEEEECCHHH
MLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNITIEMTESDAEDLKHTVDFISFSYY
HHHHHHCCCCEEEEECEECCCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHH
MTGCVSHDESINKNAQGNILNMIPNPHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIV
HHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCEEEE
ENGLGAKDSVEADGSIQDDYRIAYLNDHLVQVNEAIADGVYIMGYTSWGPIDLVSASHSQ
ECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCEEEEEECCCCCEEEECCCHHH
MSKRYGFIYVDRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKKITIKAP
HHHHCCEEEEECCCCCCCCEEHHHHHHCHHHHHHHHHCCCEEEEEEEECC
>Mature Secondary Structure
MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKMEPRILGKENIKDVAID
CCCCCCHHCCCCCCCCHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
FYHRYSEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAGLAFYDRLFDEMAQAGIKP
HHHHHHHHHHHHHHHCCEEEEEEEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
LVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRYQHKVALWLTFNEINMSLHAP
EEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECEEEECC
FTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLPEAKIGNMLLGGLVYPLTCQPQD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCEEEEEECCHHH
MLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNITIEMTESDAEDLKHTVDFISFSYY
HHHHHHCCCCEEEEECEECCCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHH
MTGCVSHDESINKNAQGNILNMIPNPHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIV
HHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCEEEE
ENGLGAKDSVEADGSIQDDYRIAYLNDHLVQVNEAIADGVYIMGYTSWGPIDLVSASHSQ
ECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCEEEEEECCCCCEEEECCCHHH
MSKRYGFIYVDRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKKITIKAP
HHHHCCEEEEECCCCCCCCEEHHHHHHCHHHHHHHHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3034860; 7686882; 9278503; 3309161 [H]