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The map label for this gene is insQ [H]

Identifier: 157163159

GI number: 157163159

Start: 3886058

End: 3887266

Strand: Reverse

Name: insQ [H]

Synonym: EcHS_A3890

Alternate gene names: 157163159

Gene position: 3887266-3886058 (Counterclockwise)

Preceding gene: 157163160

Following gene: 157163158

Centisome position: 83.71

GC content: 51.28

Gene sequence:

>1209_bases
GTGAAACGATTACAGGCATTTAAATTCCAGTTAAGACCAGGTGGTCAACAGGAGTGTGAAATGAGGCGCTTCGCAGGCGC
ATGTCGTTTCGTTTTCAATCGTGCTCTGGCACGTCAGAATGAGAATCATGAGGCCGGTAATAAATACATCCCTTACGGGA
AAATGGCTTCCTGGCTGGTTGAGTGGAAAAATGCCACTGAAACGCAATGGCTTAAAGATTCTCCCTCACAGCCATTGCAA
CAGTCACTGAAAGACCTTGAGCGGGCTTACAAAAACTTCTTCCGGAAGCGGGCTGCTTTTCCCCGATTCAAAAAGCGGGG
ACAGAATGATGCATTCCGCTACCCGCAGGGCGTTAAGCTCGATCAGGAAAACAGCCGTATTTTTCTGCCGAAACTGGGCT
GGATGCGCTACCGGAATAGCCGTCAGGTCACGGGTGTTGTGAAAAATGTCACTGTCAGCCAGTCCTGCGGTAAGTGGTAC
ATCAGTATTCAGACAGAAAGTGAAGTATCCACTCCGGCTCACCCTTCAGCATCAATGGTCAGGCTGGATGCTGGCGTGGC
TAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTAGCGAGAC
TTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCC
TGTATCGCAAATATCCGCAGGGACTACCTTCACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGA
GGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAG
GTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGCTCTGGCGTGGCGGT
CAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACTGTGGTCATACAGCGAAAGAGAACCGCCTGTC
ACAAAGTAAATTCAGATGCCAGGTATGTGGATATACAGCGAACGCCGATGTAAATGGCGCTCGTAACATTTTAGCGGCGG
GGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCG
ACAGCCTGA

Upstream 100 bases:

>100_bases
TCTGGTGACAAAGACCAGGTGAACATGCATCAGGAAAACACAATGCCTTCCCCGTCGGATATCGGTTTCTTTATTCATAG
ACCAAAGTATCATTTGGCCT

Downstream 100 bases:

>100_bases
ACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATTTGCGCTATGATACCCATCTGACCTCCTTGTAGCGG
ATGGCAAAAGCATATCAGGA

Product: IS605 family transposase OrfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQ
QSLKDLERAYKNFFRKRAAFPRFKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
ISIQTESEVSTPAHPSASMVRLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHS
CIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGG
QVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQA
TA

Sequences:

>Translated_402_residues
MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQ
QSLKDLERAYKNFFRKRAAFPRFKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
ISIQTESEVSTPAHPSASMVRLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHS
CIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGG
QVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQA
TA
>Mature_402_residues
MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQ
QSLKDLERAYKNFFRKRAAFPRFKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
ISIQTESEVSTPAHPSASMVRLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHS
CIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGG
QVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQA
TA

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG0675

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=382, Percent_Identity=98.6910994764398, Blast_Score=787, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 45824; Mature: 45824

Theoretical pI: Translated: 10.90; Mature: 10.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMASWLV
CCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
EWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPRFKKRGQNDAFRYPQGVKL
HHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCC
DQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPAHPSASMV
CCCCCEEEEECCHHHHHCCCCHHHHHHHHCEECCCCCEEEEEEEECCCCCCCCCCCCCEE
RLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHS
EECCCHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSIL
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHH
DQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGYTA
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHCEEEEECEEC
NADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMASWLV
CCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
EWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPRFKKRGQNDAFRYPQGVKL
HHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCC
DQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPAHPSASMV
CCCCCEEEEECCHHHHHCCCCHHHHHHHHCEECCCCCEEEEEEEECCCCCCCCCCCCCEE
RLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHS
EECCCHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSIL
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHH
DQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGYTA
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHCEEEEECEEC
NADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]