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The map label for this gene is yiaJ

Identifier: 157163052

GI number: 157163052

Start: 3770987

End: 3771799

Strand: Reverse

Name: yiaJ

Synonym: EcHS_A3778

Alternate gene names: 157163052

Gene position: 3771799-3770987 (Counterclockwise)

Preceding gene: 157163062

Following gene: 157163051

Centisome position: 81.23

GC content: 52.64

Gene sequence:

>813_bases
ATGGCGCAGGAAAAAGAGCGTCCAGCCGGAAGCCAGAGTCTGTTTCGCGGGTTGATGCTGATTGAGATTTTGAGCAACTA
TCCAAACGGTTGTCCGTTGGCGCATCTTTCGGAGCTGGCTGGTTTAAATAAGAGTACCGTCCATCGCTTATTGCAGGGAT
TACAGTCCTGCGGCTATGTGACCACCGCGCCTGCCGCAGGGAGTTATCGCCTGACCACCAAATTTATTGCCGTCGGGCAG
AAGGCGCTGTCTTCGCTGAATATCATTCATATCGCCGCTCCGCATCTTGAGGCACTGAACATCGCCACTGGTGAAACCAT
TAACTTCTCCAGCCGCGAAGACGATCACGCTATTTTGATTTATAAGCTGGAACCCACAACCGGGATGCTGCGAACCCGTG
CCTATATTGGCCAGCATATGCCGCTCTACTGTTCCGCAATGGGCAAGATCTACATGGCGTTTGGTCACCCGGACTACGTG
AAGTCATACTGGGAAAGCCATCAGCATGAGATCCAGCCGTTAACCCGCAATACCATTACCGAGCTGCCCGCGATGTTCGA
CGAACTGGCGCACATTCGTGAAAGCGGAGCGGCGATGGACAGAGAAGAAAACGAACTCGGCGTCTCCTGTATTGCTGTTC
CGGTGTTTGATATTCATGGGCGGGTGCCGTACGCCGTGTCGATTTCGCTTTCGACATCACGTCTGAAACAGGTGGGAGAG
AAAAATCTCCTGAAACCACTGCGTGAAACCGCGCAGGCTATTTCTAATGAACTGGGATTTACTGTCAGGGATGACCTGGG
CGCGATCACATAA

Upstream 100 bases:

>100_bases
TATAAATTATCTGTCGAGATCGTGAACTACGGCACACTTTGCGCTACCATCAGGACGCGACAAAATGGGGAAAGAAGTGA
TGGGAAAAAAAGAGAACGAG

Downstream 100 bases:

>100_bases
CGCTTTGGACAAGTGCCAAAACTTTAACATTTCCTTCGTTGGATCAAAGCAGTAGGGACGCGCTCTCTGGCACTCTGCTG
TTTTAGTGCAAAGGAGTGAT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQ
KALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYV
KSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGE
KNLLKPLRETAQAISNELGFTVRDDLGAIT

Sequences:

>Translated_270_residues
MAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQ
KALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYV
KSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGE
KNLLKPLRETAQAISNELGFTVRDDLGAIT
>Mature_269_residues
AQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQK
ALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYVK
SYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEK
NLLKPLRETAQAISNELGFTVRDDLGAIT

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain

Homologues:

Organism=Escherichia coli, GI1789999, Length=270, Percent_Identity=100, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1786468, Length=251, Percent_Identity=33.4661354581673, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1788131, Length=228, Percent_Identity=34.2105263157895, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1786715, Length=251, Percent_Identity=29.8804780876494, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082359, Length=227, Percent_Identity=33.4801762114537, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790752, Length=219, Percent_Identity=30.5936073059361, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788581, Length=238, Percent_Identity=26.890756302521, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693096, Length=143, Percent_Identity=32.1678321678322, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YIAJ_ECOLI (P37671)

Other databases:

- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S47795
- RefSeq:   AP_004218.1
- RefSeq:   NP_418031.1
- PDB:   1YSQ
- PDBsum:   1YSQ
- ProteinModelPortal:   P37671
- SMR:   P37671
- DIP:   DIP-12404N
- MINT:   MINT-1308441
- STRING:   P37671
- EnsemblBacteria:   EBESCT00000001798
- EnsemblBacteria:   EBESCT00000015306
- GeneID:   948084
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3546
- KEGG:   eco:b3574
- EchoBASE:   EB2186
- EcoGene:   EG12278
- eggNOG:   COG1414
- GeneTree:   EBGT00050000008790
- HOGENOM:   HBG739407
- OMA:   HAIMIYK
- ProtClustDB:   CLSK870422
- BioCyc:   EcoCyc:EG12278-MONOMER
- Genevestigator:   P37671
- GO:   GO:0006350
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00346

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR

EC number: NA

Molecular weight: Translated: 29705; Mature: 29574

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS51077 HTH_ICLR; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCE
TTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILI
EECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHEEECCCCCEECCCCCCCCCEEEE
YKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYVKSYWESHQHEIQPLTRNTIT
EEECCCHHHHHHHHHHCCCCCHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHH
ELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGE
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHH
KNLLKPLRETAQAISNELGFTVRDDLGAIT
HHHHHHHHHHHHHHHHHCCCEECCCCCCCC
>Mature Secondary Structure 
AQEKERPAGSQSLFRGLMLIEILSNYPNGCPLAHLSELAGLNKSTVHRLLQGLQSCGYV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCE
TTAPAAGSYRLTTKFIAVGQKALSSLNIIHIAAPHLEALNIATGETINFSSREDDHAILI
EECCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHEEECCCCCEECCCCCCCCCEEEE
YKLEPTTGMLRTRAYIGQHMPLYCSAMGKIYMAFGHPDYVKSYWESHQHEIQPLTRNTIT
EEECCCHHHHHHHHHHCCCCCHHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHH
ELPAMFDELAHIRESGAAMDREENELGVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGE
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHH
KNLLKPLRETAQAISNELGFTVRDDLGAIT
HHHHHHHHHHHHHHHHHCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503