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The map label for this gene is ysaA [H]

Identifier: 157163051

GI number: 157163051

Start: 3770412

End: 3770885

Strand: Reverse

Name: ysaA [H]

Synonym: EcHS_A3777

Alternate gene names: 157163051

Gene position: 3770885-3770412 (Counterclockwise)

Preceding gene: 157163052

Following gene: 157163047

Centisome position: 81.21

GC content: 53.8

Gene sequence:

>474_bases
ATGAACCGGTTTATTATTGCGGATGCGACGAAATGTATCGGTTGCCGTACCTGTGAAGTGGCTTGCGCAGTGTCGCATCA
GGAGAATCAGGATTGCGCTGCGTTGTCACCAGACGAGTTTATTTCCCGTATTCGTGTCATTAAAGACCACAGCTGGACCA
CGGCAGTAGCCTGTCATCAGTGTGAAGATGCACCGTGCGCGAATGTCTGCCCTGTTGACGCGATAAGCCGCGAACAAGGG
CATATTTTCGTTGAACAAACACGTTGCATTGGCTGTAAAAGCTGTATGCTGGCTTGCCCGTTTGGTGCGATGGAGGTCGT
TTCTTCGCGCAAAAAGGCGAGGGCGATTAAGTGCGATCTGTGCTGGCATCGGGAGACGGGACCGGCCTGTGTTGAAGCCT
GCCCGACAAAGGCGTTGCAGTGCATGGATGTCGAGAAAGTGCAGCGGCATCGGCTACGGCAGCAGCCTGTTTGA

Upstream 100 bases:

>100_bases
GCTTTGGACAAGTGCCAAAACTTTAACATTTCCTTCGTTGGATCAAAGCAGTAGGGACGCGCTCTCTGGCACTCTGCTGT
TTTAGTGCAAAGGAGTGATC

Downstream 100 bases:

>100_bases
AACGTTTTCCGGCGTTTCTCAACGCCGGAATAGCGTGCTTTGTTTATGCCAGATGCGGCGTAAACGCCCTATCCGGCCTA
CAAATTCTTCCAAATTCAAT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHSWTTAVACHQCEDAPCANVCPVDAISREQG
HIFVEQTRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV

Sequences:

>Translated_157_residues
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHSWTTAVACHQCEDAPCANVCPVDAISREQG
HIFVEQTRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV
>Mature_157_residues
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHSWTTAVACHQCEDAPCANVCPVDAISREQG
HIFVEQTRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367245, Length=157, Percent_Identity=98.0891719745223, Blast_Score=312, Evalue=7e-87,
Organism=Escherichia coli, GI1789067, Length=162, Percent_Identity=62.3456790123457, Blast_Score=199, Evalue=5e-53,
Organism=Escherichia coli, GI1788811, Length=147, Percent_Identity=52.3809523809524, Blast_Score=147, Evalue=2e-37,
Organism=Escherichia coli, GI87082179, Length=153, Percent_Identity=46.4052287581699, Blast_Score=140, Evalue=3e-35,
Organism=Escherichia coli, GI87082180, Length=156, Percent_Identity=44.8717948717949, Blast_Score=135, Evalue=1e-33,
Organism=Escherichia coli, GI1789079, Length=187, Percent_Identity=39.0374331550802, Blast_Score=129, Evalue=8e-32,
Organism=Escherichia coli, GI87082114, Length=190, Percent_Identity=33.6842105263158, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787872, Length=165, Percent_Identity=32.7272727272727, Blast_Score=91, Evalue=3e-20,
Organism=Escherichia coli, GI1787122, Length=163, Percent_Identity=31.9018404907975, Blast_Score=91, Evalue=3e-20,
Organism=Escherichia coli, GI226510944, Length=144, Percent_Identity=36.8055555555556, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1787963, Length=161, Percent_Identity=31.055900621118, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI2367345, Length=172, Percent_Identity=31.9767441860465, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17425; Mature: 17425

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
14.0 %Cys+Met (Translated Protein)
11.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
14.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHSWTTAVACHQ
CCEEEEECCCHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHC
CEDAPCANVCPVDAISREQGHIFVEQTRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDL
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHCEEEEEE
CWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRIRVIKDHSWTTAVACHQ
CCEEEEECCCHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHC
CEDAPCANVCPVDAISREQGHIFVEQTRCIGCKSCMLACPFGAMEVVSSRKKARAIKCDL
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHCEEEEEE
CWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV
EECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]