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The map label for this gene is tkrA [H]

Identifier: 157163028

GI number: 157163028

Start: 3746613

End: 3747587

Strand: Direct

Name: tkrA [H]

Synonym: EcHS_A3754

Alternate gene names: 157163028

Gene position: 3746613-3747587 (Clockwise)

Preceding gene: 157163027

Following gene: 157163030

Centisome position: 80.68

GC content: 52.21

Gene sequence:

>975_bases
ATGAAGCCGTCCGTTATCCTCTACAAAGCCTTACCTGATGATTTACTGCAACGCCTGCAAGAGCATTTCACCGTTCACCA
GGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACG
AGAATGTTGATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTT
GATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACGCCAACCGTATTAACAGAAACCGTCGCCGATACGCT
GATGGCGCTGGTGTTGTCTACCGCTCGTCGGGTTGTGGAGGTAGCAGAACGGGTAAAAGCAGGCGGATGGACCGCGAGCA
TAGGCCCGGACTGGTACGGCACTGACGTTCACCATAAAACACTGGGCATTGTCGGGATGGGACGGATCGGCATGGCGCTG
GCACAACGTGCGCACTTTGGCTTCAACATGCCCATCCTCTATAACGCGCGCCGCCACCATAAAGAAGCAGAAGAACGCTT
CAACGCCCGCTACTGCGATTTGGATACTCTGTTACAAGAGTCAGATTTCGTTTGCCTGATCCTGCCGTTAACTGATGAGA
CGCATCATCTGTTTGGCGCAGAACAATTCGCCAAAATGAAATCCTCCGCCATTTTCATTAATGCCGGACGTGGCCCGGTG
GTTGACGAAAATGCACTGATCGCAGCATTGCAGAAAGGCGAAATTCACGCTGCCGGGCTGGATGTCTTCGAACAAGAGCC
ACTGTCCGTAGATTCGCCGTTGCTCTCAATGGCCAACGTCGTCGCAGTACCGCATATTGGATCTGCCACCCATGAGACGC
GTTATGGCATGGCCGCCTGTGCCGTGGATAATTTGATTGATGCGTTACAAGGAAAGGTTGAGAAGAACTGTGTGAATCCG
CACGTCGCGGACTAA

Upstream 100 bases:

>100_bases
CTTTCATGCCAGGTGATGCAGAAATCACCTGGCATTCTTCAGTTTTCCTTCATCATATTTCAGGCTAAGGTGATCGCCTT
ATCAGTGAATGGAGAGAAGC

Downstream 100 bases:

>100_bases
GCCGCGACTGCGTAGAGTAAAGCCCGATAATCGCTCGGGCTTTTACTCTTTATTGGGTTGCAGTAACTGCTGTAGTCCAG
GCCTGATTAAACGCCTGATG

Product: 2-ketogluconate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNF
DVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGGWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNP
HVAD

Sequences:

>Translated_324_residues
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNF
DVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGGWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNP
HVAD
>Mature_324_residues
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNF
DVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVEVAERVKAGGWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNP
HVAD

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=273, Percent_Identity=42.4908424908425, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI23308577, Length=312, Percent_Identity=30.7692307692308, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI145580578, Length=268, Percent_Identity=32.089552238806, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4557499, Length=268, Percent_Identity=32.089552238806, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI145580575, Length=274, Percent_Identity=32.4817518248175, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4557497, Length=270, Percent_Identity=32.2222222222222, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI61743967, Length=270, Percent_Identity=32.2222222222222, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI87082289, Length=324, Percent_Identity=99.3827160493827, Blast_Score=665, Evalue=0.0,
Organism=Escherichia coli, GI1787645, Length=269, Percent_Identity=27.5092936802974, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789279, Length=249, Percent_Identity=30.9236947791165, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081824, Length=258, Percent_Identity=27.5193798449612, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=250, Percent_Identity=33.6, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=274, Percent_Identity=30.2919708029197, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=243, Percent_Identity=38.6831275720165, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320925, Length=256, Percent_Identity=32.421875, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=248, Percent_Identity=31.4516129032258, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6325144, Length=198, Percent_Identity=31.8181818181818, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324964, Length=240, Percent_Identity=26.6666666666667, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321253, Length=279, Percent_Identity=23.6559139784946, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45552429, Length=312, Percent_Identity=39.1025641025641, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28574284, Length=312, Percent_Identity=39.1025641025641, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45551003, Length=312, Percent_Identity=39.1025641025641, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24585514, Length=312, Percent_Identity=39.1025641025641, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28574282, Length=312, Percent_Identity=39.1025641025641, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28574286, Length=315, Percent_Identity=37.7777777777778, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI28571528, Length=261, Percent_Identity=42.1455938697318, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24585516, Length=247, Percent_Identity=36.0323886639676, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24646446, Length=270, Percent_Identity=35.5555555555556, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646448, Length=270, Percent_Identity=35.5555555555556, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646452, Length=270, Percent_Identity=35.5555555555556, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646450, Length=270, Percent_Identity=35.5555555555556, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI62472511, Length=270, Percent_Identity=35.5555555555556, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19921140, Length=251, Percent_Identity=30.6772908366534, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35325; Mature: 35325

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAAL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCCCCHHHH
LEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVE
HHHCCHHCCCEEEEECCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGGWTASIGPDWYGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNARRHH
HHHHHHCCCEEECCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHCCCCCCEEECCHHHH
KEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
HHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHHCCCEEEEECCCCCC
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAAC
CCCCHHEEEHHCCCEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AVDNLIDALQGKVEKNCVNPHVAD
HHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFAEAEGLLGSNENVDAAL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCCCCHHHH
LEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTETVADTLMALVLSTARRVVE
HHHCCHHCCCEEEEECCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VAERVKAGGWTASIGPDWYGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYNARRHH
HHHHHHCCCEEECCCCCCCCCCCCHHHCCEEECCHHHHHHHHHHHCCCCCCEEECCHHHH
KEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPV
HHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHHCCCEEEEECCCCCC
VDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAAC
CCCCHHEEEHHCCCEEECCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AVDNLIDALQGKVEKNCVNPHVAD
HHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA